ID   TIM22_YARLI             Reviewed;         185 AA.
AC   Q6BZY4;
DT   21-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT   10-FEB-2009, sequence version 2.
DT   25-MAY-2022, entry version 78.
DE   RecName: Full=Mitochondrial import inner membrane translocase subunit TIM22;
GN   Name=TIM22; OrderedLocusNames=YALI0F29931g;
OS   Yarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Dipodascaceae; Yarrowia.
OX   NCBI_TaxID=284591;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CLIB 122 / E 150;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Essential core component of the TIM22 complex, a complex that
CC       mediates the import and insertion of multi-pass transmembrane proteins
CC       into the mitochondrial inner membrane. In the TIM22 complex, it
CC       constitutes the voltage-activated and signal-gated channel. Forms a
CC       twin-pore translocase that uses the membrane potential as external
CC       driving force in 2 voltage-dependent steps (By similarity).
CC       {ECO:0000250|UniProtKB:Q12328}.
CC   -!- SUBUNIT: Component of the TIM22 complex, whose core is composed of
CC       TIM22 and TIM54, associated with the 70 kDa heterohexamer composed of
CC       TIM9 and TIM10 (or TIM8 and TIM13). {ECO:0000250|UniProtKB:Q12328}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC       {ECO:0000250|UniProtKB:Q12328}; Multi-pass membrane protein
CC       {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the Tim17/Tim22/Tim23 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAG78841.2; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; CR382132; CAG78841.2; ALT_INIT; Genomic_DNA.
DR   RefSeq; XP_506028.2; XM_506028.2.
DR   AlphaFoldDB; Q6BZY4; -.
DR   SMR; Q6BZY4; -.
DR   STRING; 4952.CAG78841; -.
DR   GeneID; 2908292; -.
DR   KEGG; yli:YALI0F29931g; -.
DR   InParanoid; Q6BZY4; -.
DR   Proteomes; UP000001300; Chromosome F.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0042721; C:TIM22 mitochondrial import inner membrane insertion complex; IBA:GO_Central.
DR   GO; GO:0030943; F:mitochondrion targeting sequence binding; IBA:GO_Central.
DR   GO; GO:0008320; F:protein transmembrane transporter activity; IBA:GO_Central.
DR   GO; GO:0045039; P:protein insertion into mitochondrial inner membrane; IBA:GO_Central.
DR   InterPro; IPR039175; TIM22.
DR   PANTHER; PTHR14110; PTHR14110; 1.
PE   3: Inferred from homology;
KW   Disulfide bond; Membrane; Mitochondrion; Mitochondrion inner membrane;
KW   Protein transport; Reference proteome; Translocation; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..185
FT                   /note="Mitochondrial import inner membrane translocase
FT                   subunit TIM22"
FT                   /id="PRO_0000228093"
FT   TRANSMEM        47..67
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        130..150
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        151..171
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DISULFID        42..119
FT                   /evidence="ECO:0000250|UniProtKB:A0A1D8PI78"
FT   DISULFID        138..157
FT                   /evidence="ECO:0000250|UniProtKB:A0A1D8PI78"
SQ   SEQUENCE   185 AA;  19075 MW;  5B47839C37C977DF CRC64;
     MSAFGFPGGG APTPPQGSFW DMTPEQQGMY SANLIMGTMQ SCPGKSVMAG VTGFGLGGVF
     GLFMASMAYD APVGMGVQTM SDLPFKQQMK IQFTDMGKRA WSSAKNFGFI GGVFSGTECC
     IESLRAKNDI WNGVAAGCLT GGGLAVKAGP QAALVGCAGF AAFSAAIDVY MRSDNKAPPS
     TDEDL