ID   TIM23_DANRE             Reviewed;         208 AA.
AC   Q7T2P6; Q8JHG5;
DT   05-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2003, sequence version 1.
DT   03-AUG-2022, entry version 96.
DE   RecName: Full=Mitochondrial import inner membrane translocase subunit Tim23;
GN   Name=timm23;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955 {ECO:0000312|EMBL:AAH53170.1};
RN   [1] {ECO:0000312|EMBL:AAM34675.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], FUNCTION, AND DISRUPTION PHENOTYPE.
RC   TISSUE=Embryo {ECO:0000269|PubMed:12006978};
RX   PubMed=12006978; DOI=10.1038/ng896;
RA   Golling G., Amsterdam A., Sun Z., Antonelli M., Maldonado E., Chen W.,
RA   Burgess S., Haldi M., Artzt K., Farrington S., Lin S.-Y., Nissen R.M.,
RA   Hopkins N.;
RT   "Insertional mutagenesis in zebrafish rapidly identifies genes essential
RT   for early vertebrate development.";
RL   Nat. Genet. 31:135-140(2002).
RN   [2] {ECO:0000312|EMBL:AAH53170.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Kidney {ECO:0000312|EMBL:AAH53170.1};
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Essential component of the TIM23 complex, a complex that
CC       mediates the translocation of transit peptide-containing proteins
CC       across the mitochondrial inner membrane (By similarity). Plays an
CC       essential role in early embryonic development.
CC       {ECO:0000250|UniProtKB:O14925, ECO:0000269|PubMed:12006978}.
CC   -!- SUBUNIT: Component of the TIM23 complex at least composed of timm23,
CC       timm17 and timm50. The complex interacts with the timm44 component of
CC       the PAM complex (By similarity). {ECO:0000250|UniProtKB:O14925}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC       {ECO:0000250|UniProtKB:O14925}; Multi-pass membrane protein
CC       {ECO:0000255}.
CC   -!- DISRUPTION PHENOTYPE: Mutants show various developmental defects
CC       including reduced tail circulation, and smaller head, eye and body.
CC       {ECO:0000269|PubMed:12006978}.
CC   -!- SIMILARITY: Belongs to the Tim17/Tim22/Tim23 family. {ECO:0000305}.
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DR   EMBL; AF506231; AAM34675.1; -; mRNA.
DR   EMBL; BC053170; AAH53170.1; -; mRNA.
DR   AlphaFoldDB; Q7T2P6; -.
DR   SMR; Q7T2P6; -.
DR   STRING; 7955.ENSDARP00000096296; -.
DR   PaxDb; Q7T2P6; -.
DR   ZFIN; ZDB-GENE-020419-17; timm23a.
DR   eggNOG; KOG3324; Eukaryota.
DR   InParanoid; Q7T2P6; -.
DR   PhylomeDB; Q7T2P6; -.
DR   Reactome; R-DRE-1268020; Mitochondrial protein import.
DR   PRO; PR:Q7T2P6; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Unplaced.
DR   GO; GO:0016021; C:integral component of membrane; TAS:UniProtKB.
DR   GO; GO:0031305; C:integral component of mitochondrial inner membrane; IBA:GO_Central.
DR   GO; GO:0042719; C:mitochondrial intermembrane space protein transporter complex; TAS:UniProtKB.
DR   GO; GO:0005744; C:TIM23 mitochondrial import inner membrane translocase complex; IBA:GO_Central.
DR   GO; GO:0008320; F:protein transmembrane transporter activity; IBA:GO_Central.
DR   GO; GO:0015450; F:protein-transporting ATPase activity; TAS:UniProtKB.
DR   GO; GO:0006886; P:intracellular protein transport; TAS:UniProtKB.
DR   GO; GO:0030150; P:protein import into mitochondrial matrix; IBA:GO_Central.
DR   GO; GO:0045039; P:protein insertion into mitochondrial inner membrane; TAS:UniProtKB.
DR   InterPro; IPR005681; Tim23.
DR   InterPro; IPR045238; Tim23-like.
DR   PANTHER; PTHR15371; PTHR15371; 1.
DR   TIGRFAMs; TIGR00983; 3a0801s02tim23; 1.
PE   2: Evidence at transcript level;
KW   Developmental protein; Membrane; Mitochondrion;
KW   Mitochondrion inner membrane; Protein transport; Reference proteome;
KW   Translocation; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..208
FT                   /note="Mitochondrial import inner membrane translocase
FT                   subunit Tim23"
FT                   /id="PRO_0000210305"
FT   TRANSMEM        73..93
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        125..145
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        173..193
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        4
FT                   /note="S -> N (in Ref. 1; AAM34675)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        147
FT                   /note="K -> R (in Ref. 1; AAM34675)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   208 AA;  21919 MW;  80D2189A56A26107 CRC64;
     MDNSTPPPGG FKGGLGSIFG GGTPEYSNTE LSGVPLTGMS PLSPYLNVDP RYLIQDTDEF
     ILPTGANKTR GRFELAFFTI GGCCITGAAF GTLNGLRMGL SETRDMPWSK PRNVQILNMV
     TRQGASWANT LGSVALLYSV FGVAIEKARG AEDDLNTVAA GTLTGMVFKS TGGLKGVARG
     GLIGLAMSGL YALYNNWDHL KGKSPSHY