TIM23_HUMAN
ID TIM23_HUMAN Reviewed; 209 AA.
AC O14925; Q53FF8; Q5T1E6; Q6P5S5;
DT 18-OCT-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 175.
DE RecName: Full=Mitochondrial import inner membrane translocase subunit Tim23;
GN Name=TIMM23; Synonyms=TIM23;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=10339406; DOI=10.1006/jmbi.1999.2751;
RA Bauer M.F., Gempel K., Reichert A.S., Rappold G.A., Lichtner P.,
RA Gerbitz K.-D., Neupert W., Brunner M., Hofmann S.;
RT "Genetic and structural characterization of the human mitochondrial inner
RT membrane translocase.";
RL J. Mol. Biol. 289:69-82(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Cao X., Li Q., Jin K., Liu L., Dai S., Wu G., Dong C.;
RT "Cloning of Homo sapiens translocase of inner mitochondrial membrane 23
RT (Tim23) mRNA.";
RL Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RA Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y.,
RA Tanaka A., Yokoyama S.;
RL Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15164054; DOI=10.1038/nature02462;
RA Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L.,
RA Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K.,
RA Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L.,
RA Taylor A., Battles J., Bird C.P., Ainscough R., Almeida J.P.,
RA Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J.,
RA Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y.,
RA Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P.,
RA Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N.,
RA Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A.,
RA Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C.,
RA Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D.,
RA Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C.,
RA Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K.,
RA Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A.,
RA Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S.,
RA McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S.,
RA Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V.,
RA Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A.,
RA Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M.,
RA Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A.,
RA Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P.,
RA Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y.,
RA Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D.,
RA Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J.;
RT "The DNA sequence and comparative analysis of human chromosome 10.";
RL Nature 429:375-381(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain, and Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP INTERACTION WITH TIMM50.
RX PubMed=15044455; DOI=10.1074/jbc.m402049200;
RA Guo Y., Cheong N., Zhang Z., De Rose R., Deng Y., Farber S.A.,
RA Fernandes-Alnemri T., Alnemri E.S.;
RT "Tim50, a component of the mitochondrial translocator, regulates
RT mitochondrial integrity and cell death.";
RL J. Biol. Chem. 279:24813-24825(2004).
RN [7]
RP INTERACTION WITH DNAJC15.
RX PubMed=23263864; DOI=10.1093/hmg/dds541;
RA Schusdziarra C., Blamowska M., Azem A., Hell K.;
RT "Methylation-controlled J-protein MCJ acts in the import of proteins into
RT human mitochondria.";
RL Hum. Mol. Genet. 22:1348-1357(2013).
RN [8]
RP SUBCELLULAR LOCATION.
RX PubMed=25997101; DOI=10.7554/elife.06265;
RA Guarani V., McNeill E.M., Paulo J.A., Huttlin E.L., Froehlich F.,
RA Gygi S.P., Van Vactor D., Harper J.W.;
RT "QIL1 is a novel mitochondrial protein required for MICOS complex stability
RT and cristae morphology.";
RL Elife 4:0-0(2015).
RN [9]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=25944712; DOI=10.1002/pmic.201400617;
RA Vaca Jacome A.S., Rabilloud T., Schaeffer-Reiss C., Rompais M., Ayoub D.,
RA Lane L., Bairoch A., Van Dorsselaer A., Carapito C.;
RT "N-terminome analysis of the human mitochondrial proteome.";
RL Proteomics 15:2519-2524(2015).
CC -!- FUNCTION: Essential component of the TIM23 complex, a complex that
CC mediates the translocation of transit peptide-containing proteins
CC across the mitochondrial inner membrane. {ECO:0000305|PubMed:10339406}.
CC -!- SUBUNIT: Component of the TIM23 complex at least composed of TIMM23,
CC TIMM17 (TIMM17A or TIMM17B) and TIMM50; within this complex, directly
CC interacts with TIMM50. The complex interacts with the TIMM44 component
CC of the PAM complex and with DNAJC15. {ECO:0000269|PubMed:15044455,
CC ECO:0000269|PubMed:23263864}.
CC -!- INTERACTION:
CC O14925; Q13520: AQP6; NbExp=3; IntAct=EBI-1047996, EBI-13059134;
CC O14925; Q96BA8: CREB3L1; NbExp=3; IntAct=EBI-1047996, EBI-6942903;
CC O14925; Q15125: EBP; NbExp=3; IntAct=EBI-1047996, EBI-3915253;
CC O14925; Q96KR6: FAM210B; NbExp=3; IntAct=EBI-1047996, EBI-18938272;
CC O14925; Q14802-3: FXYD3; NbExp=3; IntAct=EBI-1047996, EBI-12175685;
CC O14925; Q6ZVE7: GOLT1A; NbExp=3; IntAct=EBI-1047996, EBI-17231387;
CC O14925; Q7Z5P4: HSD17B13; NbExp=3; IntAct=EBI-1047996, EBI-18053395;
CC O14925; Q8N5M9: JAGN1; NbExp=3; IntAct=EBI-1047996, EBI-10266796;
CC O14925; P13473-2: LAMP2; NbExp=3; IntAct=EBI-1047996, EBI-21591415;
CC O14925; Q6P5S7: RNASEK; NbExp=3; IntAct=EBI-1047996, EBI-18397230;
CC O14925; Q9NY72: SCN3B; NbExp=3; IntAct=EBI-1047996, EBI-17247926;
CC O14925; Q9Y371: SH3GLB1; NbExp=3; IntAct=EBI-1047996, EBI-2623095;
CC O14925; O60669: SLC16A7; NbExp=3; IntAct=EBI-1047996, EBI-3921243;
CC O14925; O60830: TIMM17B; NbExp=4; IntAct=EBI-1047996, EBI-2372529;
CC O14925; Q9BVV7: TIMM21; NbExp=3; IntAct=EBI-1047996, EBI-6570759;
CC O14925; Q96MV1: TLCD4; NbExp=3; IntAct=EBI-1047996, EBI-12947623;
CC O14925; Q9NUH8: TMEM14B; NbExp=3; IntAct=EBI-1047996, EBI-8638294;
CC O14925; Q8N661: TMEM86B; NbExp=3; IntAct=EBI-1047996, EBI-2548832;
CC O14925; Q6PEY1: TMEM88; NbExp=3; IntAct=EBI-1047996, EBI-17198826;
CC O14925; P19075: TSPAN8; NbExp=3; IntAct=EBI-1047996, EBI-4289938;
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000269|PubMed:25997101}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the Tim17/Tim22/Tim23 family. {ECO:0000305}.
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DR EMBL; AF030162; AAB84060.1; -; mRNA.
DR EMBL; AY442341; AAR14723.1; -; mRNA.
DR EMBL; AK223331; BAD97051.1; -; mRNA.
DR EMBL; AL450342; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC062707; AAH62707.1; -; mRNA.
DR EMBL; BC066951; AAH66951.1; -; mRNA.
DR CCDS; CCDS73091.1; -.
DR RefSeq; NP_006318.1; NM_006327.3.
DR AlphaFoldDB; O14925; -.
DR SMR; O14925; -.
DR BioGRID; 939591; 77.
DR ComplexPortal; CPX-6129; TIM23 mitochondrial inner membrane pre-sequence translocase complex, TIM17A variant.
DR ComplexPortal; CPX-6130; TIM23 mitochondrial inner membrane pre-sequence translocase complex, TIM17B variant.
DR CORUM; O14925; -.
DR IntAct; O14925; 59.
DR MINT; O14925; -.
DR STRING; 9606.ENSP00000464522; -.
DR iPTMnet; O14925; -.
DR PhosphoSitePlus; O14925; -.
DR SwissPalm; O14925; -.
DR BioMuta; TIMM23; -.
DR EPD; O14925; -.
DR jPOST; O14925; -.
DR MassIVE; O14925; -.
DR MaxQB; O14925; -.
DR PaxDb; O14925; -.
DR PeptideAtlas; O14925; -.
DR PRIDE; O14925; -.
DR ProteomicsDB; 48306; -.
DR TopDownProteomics; O14925; -.
DR Antibodypedia; 73127; 108 antibodies from 25 providers.
DR DNASU; 100287932; -.
DR Ensembl; ENST00000580018.4; ENSP00000464522.3; ENSG00000265354.4.
DR GeneID; 100287932; -.
DR KEGG; hsa:100287932; -.
DR MANE-Select; ENST00000580018.4; ENSP00000464522.3; NM_006327.4; NP_006318.1.
DR UCSC; uc001jiu.5; human.
DR CTD; 100287932; -.
DR DisGeNET; 100287932; -.
DR GeneCards; TIMM23; -.
DR HGNC; HGNC:17312; TIMM23.
DR HPA; ENSG00000265354; Low tissue specificity.
DR MIM; 605034; gene.
DR neXtProt; NX_O14925; -.
DR OpenTargets; ENSG00000265354; -.
DR PharmGKB; PA38227; -.
DR VEuPathDB; HostDB:ENSG00000265354; -.
DR eggNOG; KOG3324; Eukaryota.
DR GeneTree; ENSGT00390000001094; -.
DR HOGENOM; CLU_063935_2_0_1; -.
DR InParanoid; O14925; -.
DR OMA; QYIMPEG; -.
DR OrthoDB; 1418527at2759; -.
DR PhylomeDB; O14925; -.
DR TreeFam; TF106196; -.
DR PathwayCommons; O14925; -.
DR Reactome; R-HSA-1268020; Mitochondrial protein import.
DR SignaLink; O14925; -.
DR SIGNOR; O14925; -.
DR BioGRID-ORCS; 100287932; 522 hits in 1071 CRISPR screens.
DR ChiTaRS; TIMM23; human.
DR GenomeRNAi; 100287932; -.
DR Pharos; O14925; Tbio.
DR PRO; PR:O14925; -.
DR Proteomes; UP000005640; Chromosome 10.
DR RNAct; O14925; protein.
DR Bgee; ENSG00000265354; Expressed in gingival epithelium and 206 other tissues.
DR Genevisible; O14925; HS.
DR GO; GO:0031305; C:integral component of mitochondrial inner membrane; IDA:BHF-UCL.
DR GO; GO:0005743; C:mitochondrial inner membrane; IDA:UniProtKB.
DR GO; GO:0005758; C:mitochondrial intermembrane space; IDA:MGI.
DR GO; GO:0005739; C:mitochondrion; IDA:HPA.
DR GO; GO:0005744; C:TIM23 mitochondrial import inner membrane translocase complex; IDA:BHF-UCL.
DR GO; GO:0008320; F:protein transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0006886; P:intracellular protein transport; IC:ComplexPortal.
DR GO; GO:0030150; P:protein import into mitochondrial matrix; IBA:GO_Central.
DR GO; GO:0006626; P:protein targeting to mitochondrion; TAS:ProtInc.
DR InterPro; IPR005681; Tim23.
DR InterPro; IPR045238; Tim23-like.
DR PANTHER; PTHR15371; PTHR15371; 1.
DR TIGRFAMs; TIGR00983; 3a0801s02tim23; 1.
PE 1: Evidence at protein level;
KW Membrane; Mitochondrion; Mitochondrion inner membrane; Protein transport;
KW Reference proteome; Translocation; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..209
FT /note="Mitochondrial import inner membrane translocase
FT subunit Tim23"
FT /id="PRO_0000210302"
FT TRANSMEM 73..93
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 125..145
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 181..197
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 27
FT /note="S -> P (in Ref. 3; BAD97051)"
FT /evidence="ECO:0000305"
FT CONFLICT 62
FT /note="Missing (in Ref. 5; AAH62707)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 209 AA; 21943 MW; C9A1BBEC703B6838 CRC64;
MEGGGGSGNK TTGGLAGFFG AGGAGYSHAD LAGVPLTGMN PLSPYLNVDP RYLVQDTDEF
ILPTGANKTR GRFELAFFTI GGCCMTGAAF GAMNGLRLGL KETQNMAWSK PRNVQILNMV
TRQGALWANT LGSLALLYSA FGVIIEKTRG AEDDLNTVAA GTMTGMLYKC TGGLRGIARG
GLTGLTLTSL YALYNNWEHM KGSLLQQSL
