TIM23_MOUSE
ID TIM23_MOUSE Reviewed; 209 AA.
AC Q9WTQ8;
DT 18-OCT-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 25-MAY-2022, entry version 118.
DE RecName: Full=Mitochondrial import inner membrane translocase subunit Tim23;
GN Name=Timm23; Synonyms=Tim23;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=JCL-ICR; TISSUE=Mammary gland;
RX PubMed=11165051; DOI=10.1016/s0303-7207(00)00370-1;
RA Sun Y., Kuraishi T., Aoki F., Sakai S.;
RT "Hormonal regulation of mitochondrial Tim23 gene expression in the mouse
RT mammary gland.";
RL Mol. Cell. Endocrinol. 172:177-184(2001).
RN [2]
RP DISRUPTION PHENOTYPE, AND SUBCELLULAR LOCATION.
RX PubMed=19111522; DOI=10.1016/j.bbabio.2008.12.001;
RA Ahting U., Floss T., Uez N., Schneider-Lohmar I., Becker L., Kling E.,
RA Iuso A., Bender A., de Angelis M.H., Gailus-Durner V., Fuchs H.,
RA Meitinger T., Wurst W., Prokisch H., Klopstock T.;
RT "Neurological phenotype and reduced lifespan in heterozygous Tim23 knockout
RT mice, the first mouse model of defective mitochondrial import.";
RL Biochim. Biophys. Acta 1787:371-376(2009).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas,
RC Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Essential component of the TIM23 complex, a complex that
CC mediates the translocation of transit peptide-containing proteins
CC across the mitochondrial inner membrane.
CC {ECO:0000250|UniProtKB:O14925}.
CC -!- SUBUNIT: Component of the TIM23 complex at least composed of TIMM23,
CC TIMM17 (TIMM17A or TIMM17B) and TIMM50; within this complex, directly
CC interacts with TIMM50. The complex interacts with the TIMM44 component
CC of the PAM complex and with DNAJC15. {ECO:0000250|UniProtKB:O14925}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000269|PubMed:19111522}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- DISRUPTION PHENOTYPE: Mutants are not viable (PubMed:19111522).
CC Heterozygous animals show an aneurological phenotype, premature aging
CC and reduced lifespan (PubMed:19111522). {ECO:0000269|PubMed:19111522}.
CC -!- SIMILARITY: Belongs to the Tim17/Tim22/Tim23 family. {ECO:0000305}.
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DR EMBL; AB021122; BAA78407.1; -; mRNA.
DR CCDS; CCDS36864.1; -.
DR AlphaFoldDB; Q9WTQ8; -.
DR SMR; Q9WTQ8; -.
DR IntAct; Q9WTQ8; 1.
DR MINT; Q9WTQ8; -.
DR STRING; 10090.ENSMUSP00000013845; -.
DR PhosphoSitePlus; Q9WTQ8; -.
DR EPD; Q9WTQ8; -.
DR jPOST; Q9WTQ8; -.
DR MaxQB; Q9WTQ8; -.
DR PaxDb; Q9WTQ8; -.
DR PeptideAtlas; Q9WTQ8; -.
DR PRIDE; Q9WTQ8; -.
DR MGI; MGI:1858317; Timm23.
DR eggNOG; KOG3324; Eukaryota.
DR InParanoid; Q9WTQ8; -.
DR PhylomeDB; Q9WTQ8; -.
DR ChiTaRS; Timm23; mouse.
DR PRO; PR:Q9WTQ8; -.
DR Proteomes; UP000000589; Unplaced.
DR RNAct; Q9WTQ8; protein.
DR GO; GO:0031305; C:integral component of mitochondrial inner membrane; ISO:MGI.
DR GO; GO:0005743; C:mitochondrial inner membrane; HDA:MGI.
DR GO; GO:0005758; C:mitochondrial intermembrane space; ISO:MGI.
DR GO; GO:0005739; C:mitochondrion; IDA:MGI.
DR GO; GO:0005744; C:TIM23 mitochondrial import inner membrane translocase complex; ISO:MGI.
DR GO; GO:0019899; F:enzyme binding; ISO:MGI.
DR GO; GO:0008320; F:protein transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0030150; P:protein import into mitochondrial matrix; IBA:GO_Central.
DR InterPro; IPR005681; Tim23.
DR InterPro; IPR045238; Tim23-like.
DR PANTHER; PTHR15371; PTHR15371; 1.
DR TIGRFAMs; TIGR00983; 3a0801s02tim23; 1.
PE 1: Evidence at protein level;
KW Membrane; Mitochondrion; Mitochondrion inner membrane; Protein transport;
KW Reference proteome; Translocation; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..209
FT /note="Mitochondrial import inner membrane translocase
FT subunit Tim23"
FT /id="PRO_0000210303"
FT TRANSMEM 73..93
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 125..145
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 181..197
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 209 AA; 21978 MW; CA27983963AF2E3C CRC64;
MEGGGRSSNK STSGLAGFFG AGGAGYSNAD LAGVPLTGMN PLSPYLNVDP RYLVQDTDEF
ILPTGANKTR GRFELAFFTI GGCCMTGAAF GAMNGLRLGL KETQSMAWSK PRNVQILNMV
TRQGALWANT LGSLALLYSA FGVIIEKTRG AEDDLNTVAA GTMTGMLYKC TGGLRGIARG
GLAGLTLTSL YALYNNWVHM KGSLLQQSL
