TIM29_MOUSE
ID TIM29_MOUSE Reviewed; 266 AA.
AC Q8BGX2;
DT 09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=Mitochondrial import inner membrane translocase subunit Tim29;
DE Short=TIM29;
DE Flags: Precursor;
GN Name=Timm29;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J, and NOD; TISSUE=Pituitary, Skin, and Thymus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas,
RC Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Component of the TIM22 complex, a complex that mediates the
CC import and insertion of multi-pass transmembrane proteins into the
CC mitochondrial inner membrane. The TIM22 complex forms a twin-pore
CC translocase that uses the membrane potential as the external driving
CC force. Required for the stability of the TIM22 complex and functions in
CC the assembly of the TIMM22 protein into the TIM22 complex. May
CC facilitate cooperation between TIM22 and TOM complexes by interacting
CC with TOMM40. {ECO:0000250|UniProtKB:Q9BSF4}.
CC -!- SUBUNIT: Component of the TIM22 complex, which core is composed of
CC TIMM22, associated with TIMM10 (TIMM10A and/or TIMM10B), TIMM9, AGK and
CC TIMM29. Interacts with TIMM10B; the interaction is direct. Interacts
CC with TOMM40; linking the TIM22 complex to the TOM complex. Interacts
CC with TIMM22 (when oxidized); the interaction is direct.
CC {ECO:0000250|UniProtKB:Q9BSF4}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000250|UniProtKB:Q9BSF4}; Single-pass membrane protein
CC {ECO:0000250|UniProtKB:Q9BSF4}; Intermembrane side
CC {ECO:0000250|UniProtKB:Q9BSF4}.
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DR EMBL; AK028799; BAC26126.1; -; mRNA.
DR EMBL; AK030602; BAC27040.1; -; mRNA.
DR EMBL; AK088045; BAC40114.1; -; mRNA.
DR EMBL; BC057071; AAH57071.1; -; mRNA.
DR CCDS; CCDS22908.1; -.
DR RefSeq; NP_848734.1; NM_178619.4.
DR AlphaFoldDB; Q8BGX2; -.
DR SMR; Q8BGX2; -.
DR BioGRID; 213670; 1.
DR STRING; 10090.ENSMUSP00000058283; -.
DR iPTMnet; Q8BGX2; -.
DR PhosphoSitePlus; Q8BGX2; -.
DR SwissPalm; Q8BGX2; -.
DR EPD; Q8BGX2; -.
DR jPOST; Q8BGX2; -.
DR MaxQB; Q8BGX2; -.
DR PaxDb; Q8BGX2; -.
DR PeptideAtlas; Q8BGX2; -.
DR PRIDE; Q8BGX2; -.
DR ProteomicsDB; 259507; -.
DR Antibodypedia; 51917; 34 antibodies from 13 providers.
DR DNASU; 69773; -.
DR Ensembl; ENSMUST00000062125; ENSMUSP00000058283; ENSMUSG00000048429.
DR GeneID; 69773; -.
DR KEGG; mmu:69773; -.
DR UCSC; uc009omb.2; mouse.
DR CTD; 90580; -.
DR MGI; MGI:1917023; Timm29.
DR VEuPathDB; HostDB:ENSMUSG00000048429; -.
DR eggNOG; KOG4545; Eukaryota.
DR GeneTree; ENSGT00390000018541; -.
DR HOGENOM; CLU_102697_0_0_1; -.
DR InParanoid; Q8BGX2; -.
DR OMA; WRLKWKM; -.
DR OrthoDB; 1386679at2759; -.
DR PhylomeDB; Q8BGX2; -.
DR TreeFam; TF313304; -.
DR BioGRID-ORCS; 69773; 29 hits in 73 CRISPR screens.
DR PRO; PR:Q8BGX2; -.
DR Proteomes; UP000000589; Chromosome 9.
DR RNAct; Q8BGX2; protein.
DR Bgee; ENSMUSG00000048429; Expressed in triceps brachii and 248 other tissues.
DR Genevisible; Q8BGX2; MM.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005743; C:mitochondrial inner membrane; ISS:UniProtKB.
DR GO; GO:0005758; C:mitochondrial intermembrane space; ISO:MGI.
DR GO; GO:0042721; C:TIM22 mitochondrial import inner membrane insertion complex; ISS:UniProtKB.
DR GO; GO:0140318; F:protein transporter activity; ISO:MGI.
DR GO; GO:0045039; P:protein insertion into mitochondrial inner membrane; ISS:UniProtKB.
DR InterPro; IPR019322; TIMM29.
DR PANTHER; PTHR21435; PTHR21435; 1.
DR Pfam; PF10171; Tim29; 1.
PE 1: Evidence at protein level;
KW Membrane; Mitochondrion; Mitochondrion inner membrane; Protein transport;
KW Reference proteome; Transit peptide; Translocation; Transmembrane;
KW Transmembrane helix; Transport.
FT TRANSIT 1..37
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN 38..266
FT /note="Mitochondrial import inner membrane translocase
FT subunit Tim29"
FT /id="PRO_0000271007"
FT TOPO_DOM 38..65
FT /note="Mitochondrial matrix"
FT /evidence="ECO:0000250|UniProtKB:Q9BSF4"
FT TRANSMEM 66..83
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 84..266
FT /note="Mitochondrial intermembrane"
FT /evidence="ECO:0000250|UniProtKB:Q9BSF4"
SQ SEQUENCE 266 AA; 29415 MW; 66FE7EAD6ACE28B9 CRC64;
MVTAALKRFW SGGHGEAGGE AGGATTVAVK PGLWTRLSTW AGALLRDYAE ACGDAAAAAR
ARPGRAALYV GLLGGAAACC ALAPSEAAFE EALLDASGSL LLLAPATRNR HSEAFLQRLL
WLRGRGRLRH VNLGFCSLVY EAPFDAQASL YQARCRYLQP RWVDFPGRIL DVGFVGRWWI
LQNRMHDCDI NDDEFLHLPA HLRVVAPHQL HSEANERLFE EKYKPIILTD DQVDQALWEE
QVLQKERKDR LALSEADSLV QSDVSR
