ID   TIM44_CAEEL             Reviewed;         425 AA.
AC   O02161;
DT   15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1997, sequence version 1.
DT   03-AUG-2022, entry version 138.
DE   RecName: Full=Probable mitochondrial import inner membrane translocase subunit tin-44 {ECO:0000312|WormBase:T09B4.9};
DE   Flags: Precursor;
GN   Name=tin-44 {ECO:0000312|WormBase:T09B4.9};
GN   ORFNames=T09B4.9 {ECO:0000312|WormBase:T09B4.9};
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6239;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2;
RX   PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for investigating
RT   biology.";
RL   Science 282:2012-2018(1998).
CC   -!- FUNCTION: Essential component of the PAM complex, a complex required
CC       for the translocation of transit peptide-containing proteins from the
CC       inner membrane into the mitochondrial matrix in an ATP-dependent
CC       manner. Recruits mitochondrial HSP70 to drive protein translocation
CC       into the matrix using ATP as an energy source.
CC       {ECO:0000250|UniProtKB:Q07914}.
CC   -!- SUBUNIT: Probable component of the PAM complex at least composed of a
CC       mitochondrial HSP70 protein, GrpE, tin-44, tim-16 and tim-14/dnj-21.
CC       The complex interacts with the tim-23 component of the TIM23 complex.
CC       {ECO:0000250|UniProtKB:Q07914}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the Tim44 family. {ECO:0000305}.
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DR   EMBL; FO081495; CCD71993.1; -; Genomic_DNA.
DR   PIR; T25873; T25873.
DR   RefSeq; NP_491780.1; NM_059379.3.
DR   AlphaFoldDB; O02161; -.
DR   SMR; O02161; -.
DR   BioGRID; 37755; 14.
DR   DIP; DIP-24454N; -.
DR   IntAct; O02161; 1.
DR   STRING; 6239.T09B4.9; -.
DR   EPD; O02161; -.
DR   PaxDb; O02161; -.
DR   PeptideAtlas; O02161; -.
DR   EnsemblMetazoa; T09B4.9.1; T09B4.9.1; WBGene00020383.
DR   GeneID; 172302; -.
DR   KEGG; cel:CELE_T09B4.9; -.
DR   UCSC; T09B4.9; c. elegans.
DR   CTD; 172302; -.
DR   WormBase; T09B4.9; CE13473; WBGene00020383; tin-44.
DR   eggNOG; KOG2580; Eukaryota.
DR   GeneTree; ENSGT00390000000051; -.
DR   HOGENOM; CLU_020932_1_1_1; -.
DR   InParanoid; O02161; -.
DR   OMA; PVFVVTC; -.
DR   OrthoDB; 1041560at2759; -.
DR   PhylomeDB; O02161; -.
DR   SignaLink; O02161; -.
DR   PRO; PR:O02161; -.
DR   Proteomes; UP000001940; Chromosome I.
DR   Bgee; WBGene00020383; Expressed in germ line (C elegans) and 4 other tissues.
DR   GO; GO:0005743; C:mitochondrial inner membrane; IBA:GO_Central.
DR   GO; GO:0051087; F:chaperone binding; IBA:GO_Central.
DR   GO; GO:0030150; P:protein import into mitochondrial matrix; IBA:GO_Central.
DR   InterPro; IPR032710; NTF2-like_dom_sf.
DR   InterPro; IPR017303; Tim44.
DR   InterPro; IPR039544; Tim44-like.
DR   InterPro; IPR007379; Tim44-like_dom.
DR   PANTHER; PTHR10721; PTHR10721; 1.
DR   Pfam; PF04280; Tim44; 1.
DR   PIRSF; PIRSF037871; TIM44; 1.
DR   SMART; SM00978; Tim44; 1.
DR   SUPFAM; SSF54427; SSF54427; 1.
DR   TIGRFAMs; TIGR00984; 3a0801s03tim44; 1.
PE   3: Inferred from homology;
KW   Coiled coil; Membrane; Mitochondrion; Mitochondrion inner membrane;
KW   Protein transport; Reference proteome; Transit peptide; Translocation;
KW   Transport.
FT   TRANSIT         1..?
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           ?..425
FT                   /note="Probable mitochondrial import inner membrane
FT                   translocase subunit tin-44"
FT                   /id="PRO_0000034317"
FT   COILED          38..149
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   425 AA;  49398 MW;  203DFBD614E099F8 CRC64;
     MLSRICGNGI RLTRTRLQFQ PSIVTFRDYS NPAPKRGFLN NLIDNVRDEM QKNKELQEHQ
     QQLKARMQEL NESDALKDAR KKFEIVEKET LKSSEVVKQK IEELSDHMKK MVHEIQKTEA
     GKKMTEAGAE ALKQARKAAE HVEKVAEKVG DTEVYKHVST SMKTVKDEID NIADVRMYSR
     PEALTKRTDG FDLEKERVVE ANDSATDVTL HKDSKWYSGW KNFSESNTYY HKLLDWKIKY
     DESDNMAVRM MRGVTEKIGS VFSGQNEVSE VLTEIHKIDA NFDKQEWLRF CETKIIPNIL
     EAFIRFDLEV LQSWCHERAY TQLSTVVKEY QKMHFSTKDS RIIDINKVEM ATGKMMEQGP
     VLIISFQVYM INVTKNADGK VVEGDPDNPK RINHIWVLCR DVEEYNPALA WKLLEVHMQE
     TPLAL