TIM44_SCHPO
ID TIM44_SCHPO Reviewed; 427 AA.
AC O60084;
DT 15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1998, sequence version 1.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=Mitochondrial import inner membrane translocase subunit tim44;
DE Flags: Precursor;
GN Name=tim44; ORFNames=SPBC14C8.02;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
CC -!- FUNCTION: Essential component of the PAM complex, a complex required
CC for the translocation of transit peptide-containing proteins from the
CC inner membrane into the mitochondrial matrix in an ATP-dependent
CC manner. Recruits mitochondrial HSP70 and its co-chaperone (mge1) to
CC drive protein translocation into the matrix using ATP as an energy
CC source. {ECO:0000250|UniProtKB:Q01852}.
CC -!- SUBUNIT: Heterodimer with pam18. Component of the PAM complex, at least
CC composed of mtHsp70, mge1, tim44, pam16, pam17 and pam18. The complex
CC interacts with the tim23 component of the PAM complex.
CC {ECO:0000250|UniProtKB:Q01852}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000250|UniProtKB:Q01852}; Peripheral membrane protein
CC {ECO:0000250|UniProtKB:Q01852}.
CC -!- SIMILARITY: Belongs to the Tim44 family. {ECO:0000305}.
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DR EMBL; CU329671; CAA18420.1; -; Genomic_DNA.
DR PIR; T39430; T39430.
DR RefSeq; NP_595905.1; NM_001021813.2.
DR AlphaFoldDB; O60084; -.
DR SMR; O60084; -.
DR BioGRID; 276192; 2.
DR IntAct; O60084; 2.
DR STRING; 4896.SPBC14C8.02.1; -.
DR MaxQB; O60084; -.
DR PaxDb; O60084; -.
DR PRIDE; O60084; -.
DR EnsemblFungi; SPBC14C8.02.1; SPBC14C8.02.1:pep; SPBC14C8.02.
DR GeneID; 2539636; -.
DR KEGG; spo:SPBC14C8.02; -.
DR PomBase; SPBC14C8.02; tim44.
DR VEuPathDB; FungiDB:SPBC14C8.02; -.
DR eggNOG; KOG2580; Eukaryota.
DR HOGENOM; CLU_020932_2_0_1; -.
DR InParanoid; O60084; -.
DR OMA; PVFVVTC; -.
DR PhylomeDB; O60084; -.
DR PRO; PR:O60084; -.
DR Proteomes; UP000002485; Chromosome II.
DR GO; GO:0005743; C:mitochondrial inner membrane; IBA:GO_Central.
DR GO; GO:0001405; C:PAM complex, Tim23 associated import motor; ISS:PomBase.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0051087; F:chaperone binding; IBA:GO_Central.
DR GO; GO:0030150; P:protein import into mitochondrial matrix; ISS:PomBase.
DR InterPro; IPR032710; NTF2-like_dom_sf.
DR InterPro; IPR017303; Tim44.
DR InterPro; IPR039544; Tim44-like.
DR InterPro; IPR007379; Tim44-like_dom.
DR PANTHER; PTHR10721; PTHR10721; 1.
DR Pfam; PF04280; Tim44; 1.
DR PIRSF; PIRSF037871; TIM44; 1.
DR SMART; SM00978; Tim44; 1.
DR SUPFAM; SSF54427; SSF54427; 1.
DR TIGRFAMs; TIGR00984; 3a0801s03tim44; 1.
PE 3: Inferred from homology;
KW ATP-binding; Membrane; Mitochondrion; Mitochondrion inner membrane;
KW Nucleotide-binding; Protein transport; Reference proteome; Transit peptide;
KW Translocation; Transport.
FT TRANSIT 1..?
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN ?..427
FT /note="Mitochondrial import inner membrane translocase
FT subunit tim44"
FT /id="PRO_0000034318"
FT BINDING 90..97
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
SQ SEQUENCE 427 AA; 48550 MW; 22F3F1C61FEB8E47 CRC64;
MFLSRKITKN ARTIVQCQRF FQTSIFSQNA APQSPIKVFM DTFRAELKKS QELQDSVKAL
QDSSGSLSES DTFKKARDAY EKARSGTTAA SSFTGKTVGK AGAKIGSYAQ KAWESAPVQL
SKKVISSTAN TVATGVDTAT KPVRETAFYK TIKQTMSDGS TSSRYGFYAD KEQRKKLREE
FERRNRMFAS SARIQPNEDV QSVVVHSNPS WKNKVEQIKN ESRLVRKIQE LKKSYQESEH
PIVSSIRDMA DSISGVWSRM FSETEASQVM RRFKEIDPSF NTEHFLQYLR EYIVPEVTEA
YVKGDKEVLK TWLSEAPFSV YETTTKEYAK HGVVSVGKIL DIRGVDIMSQ RLLQPNDIPV
FIVTFRTQEV HMFKDASSGE LVAGKDDRIQ QCTYASVFTR VEDELDNPET RGWRIVDFAR
ARAVDYF
