TIM50_ARATH
ID TIM50_ARATH Reviewed; 376 AA.
AC Q8VYE2; Q56X01; Q9LG22;
DT 06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=Mitochondrial import inner membrane translocase subunit TIM50;
DE AltName: Full=Protein EMBRYO DEFECTIVE 1860;
DE Flags: Precursor;
GN Name=TIM50; Synonyms=EMB1860; OrderedLocusNames=At1g55900;
GN ORFNames=F14J16.15;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 233-376.
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, AND INDUCTION.
RX PubMed=14730085; DOI=10.1104/pp.103.033910;
RA Lister R., Chew O., Lee M.N., Heazlewood J.L., Clifton R., Parker K.L.,
RA Millar A.H., Whelan J.;
RT "A transcriptomic and proteomic characterization of the Arabidopsis
RT mitochondrial protein import apparatus and its response to mitochondrial
RT dysfunction.";
RL Plant Physiol. 134:777-789(2004).
RN [6]
RP INTERACTION WITH TIM23-2.
RX PubMed=22730406; DOI=10.1105/tpc.112.098731;
RA Wang Y., Carrie C., Giraud E., Elhafez D., Narsai R., Duncan O., Whelan J.,
RA Murcha M.W.;
RT "Dual location of the mitochondrial preprotein transporters B14.7 and
RT Tim23-2 in complex I and the TIM17:23 complex in Arabidopsis links
RT mitochondrial activity and biogenesis.";
RL Plant Cell 24:2675-2695(2012).
CC -!- FUNCTION: Essential component of the TIM17:23 complex, a complex that
CC mediates the translocation of transit peptide-containing proteins
CC across the mitochondrial inner membrane. {ECO:0000250}.
CC -!- SUBUNIT: Component of the TIM17:23 complex at least composed of TIM23,
CC TIM17 and TIM50. Interacts with TIM23-2. {ECO:0000269|PubMed:22730406}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000269|PubMed:14730085}; Single-pass membrane protein
CC {ECO:0000269|PubMed:14730085}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q8VYE2-1; Sequence=Displayed;
CC -!- TISSUE SPECIFICITY: Expressed in young cotyledons, roots, flowers and
CC leaves. {ECO:0000269|PubMed:14730085}.
CC -!- INDUCTION: Up-regulated after antimycin A or rotenone treatments.
CC {ECO:0000269|PubMed:14730085}.
CC -!- SIMILARITY: Belongs to the TIM50 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF79316.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=BAD94197.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AC002304; AAF79316.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE33316.1; -; Genomic_DNA.
DR EMBL; AY072140; AAL59962.1; -; mRNA.
DR EMBL; AY122975; AAM67508.1; -; mRNA.
DR EMBL; AK221878; BAD94197.1; ALT_INIT; mRNA.
DR RefSeq; NP_175986.2; NM_104467.5. [Q8VYE2-1]
DR AlphaFoldDB; Q8VYE2; -.
DR SMR; Q8VYE2; -.
DR STRING; 3702.AT1G55900.1; -.
DR SwissPalm; Q8VYE2; -.
DR PaxDb; Q8VYE2; -.
DR PRIDE; Q8VYE2; -.
DR ProteomicsDB; 232458; -. [Q8VYE2-1]
DR EnsemblPlants; AT1G55900.1; AT1G55900.1; AT1G55900. [Q8VYE2-1]
DR GeneID; 842040; -.
DR Gramene; AT1G55900.1; AT1G55900.1; AT1G55900. [Q8VYE2-1]
DR KEGG; ath:AT1G55900; -.
DR Araport; AT1G55900; -.
DR TAIR; locus:2012010; AT1G55900.
DR eggNOG; KOG2832; Eukaryota.
DR InParanoid; Q8VYE2; -.
DR OMA; KEHHRRM; -.
DR OrthoDB; 1142452at2759; -.
DR PhylomeDB; Q8VYE2; -.
DR PRO; PR:Q8VYE2; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q8VYE2; baseline and differential.
DR Genevisible; Q8VYE2; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005743; C:mitochondrial inner membrane; HDA:TAIR.
DR GO; GO:0005739; C:mitochondrion; HDA:TAIR.
DR GO; GO:0005634; C:nucleus; HDA:TAIR.
DR GO; GO:0005744; C:TIM23 mitochondrial import inner membrane translocase complex; IBA:GO_Central.
DR GO; GO:0030150; P:protein import into mitochondrial matrix; IBA:GO_Central.
DR Gene3D; 3.40.50.1000; -; 1.
DR InterPro; IPR004274; FCP1_dom.
DR InterPro; IPR036412; HAD-like_sf.
DR InterPro; IPR023214; HAD_sf.
DR Pfam; PF03031; NIF; 1.
DR SMART; SM00577; CPDc; 1.
DR SUPFAM; SSF56784; SSF56784; 1.
DR PROSITE; PS50969; FCP1; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Membrane; Mitochondrion;
KW Mitochondrion inner membrane; Protein transport; Reference proteome;
KW Transit peptide; Translocation; Transmembrane; Transmembrane helix;
KW Transport.
FT TRANSIT 1..25
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN 26..376
FT /note="Mitochondrial import inner membrane translocase
FT subunit TIM50"
FT /id="PRO_0000043124"
FT TOPO_DOM 26..84
FT /note="Mitochondrial matrix"
FT /evidence="ECO:0000255"
FT TRANSMEM 85..107
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 108..376
FT /note="Mitochondrial intermembrane"
FT /evidence="ECO:0000255"
FT DOMAIN 186..329
FT /note="FCP1 homology"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00336"
FT REGION 32..66
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 32..50
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 51..66
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 376 AA; 42640 MW; 160466DD597215AA CRC64;
MASIVLRSRL LPRLAKLRSR NLRCFSAEAS STNSTSRYSG VTSTQSMFSD FPPPNQPPPP
PPPQVEAAAA AATGKERKGL KYLGYALLWA LTGATAATGY ASFAYTIDEV NEKTKAFRES
ATKTPVIKSS GIDVIDKYQT KLYSAAMTGS ARAIDKYLEL REIVEEQVKG FTEPLSEKLL
PDLHPAEQHV FTLVLDLNET LLYTDWKRER GWRTFKRPGV DAFLEHLGKF YEIVVYSDQM
EMYVLPVCEK LDPNGYIRYK LARGATKYEN GKHYRDLSKL NRDPKKILFV SANAFESTLQ
PENSVPIKPY KLEADDTALV DLIPFLEYVA RNSPADIRPV LASFERKDIA KEFIDRSIEY
QKRKQGQLGQ GRFWRR
