TIM50_ASPFU
ID TIM50_ASPFU Reviewed; 501 AA.
AC Q4WI16;
DT 06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 06-DEC-2005, sequence version 2.
DT 03-AUG-2022, entry version 92.
DE RecName: Full=Mitochondrial import inner membrane translocase subunit tim50;
DE Flags: Precursor;
GN Name=tim50; ORFNames=AFUA_2G03420;
OS Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC
OS A1100) (Aspergillus fumigatus).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC Aspergillus subgen. Fumigati.
OX NCBI_TaxID=330879;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100;
RX PubMed=16372009; DOI=10.1038/nature04332;
RA Nierman W.C., Pain A., Anderson M.J., Wortman J.R., Kim H.S., Arroyo J.,
RA Berriman M., Abe K., Archer D.B., Bermejo C., Bennett J.W., Bowyer P.,
RA Chen D., Collins M., Coulsen R., Davies R., Dyer P.S., Farman M.L.,
RA Fedorova N., Fedorova N.D., Feldblyum T.V., Fischer R., Fosker N.,
RA Fraser A., Garcia J.L., Garcia M.J., Goble A., Goldman G.H., Gomi K.,
RA Griffith-Jones S., Gwilliam R., Haas B.J., Haas H., Harris D.E.,
RA Horiuchi H., Huang J., Humphray S., Jimenez J., Keller N., Khouri H.,
RA Kitamoto K., Kobayashi T., Konzack S., Kulkarni R., Kumagai T., Lafton A.,
RA Latge J.-P., Li W., Lord A., Lu C., Majoros W.H., May G.S., Miller B.L.,
RA Mohamoud Y., Molina M., Monod M., Mouyna I., Mulligan S., Murphy L.D.,
RA O'Neil S., Paulsen I., Penalva M.A., Pertea M., Price C., Pritchard B.L.,
RA Quail M.A., Rabbinowitsch E., Rawlins N., Rajandream M.A., Reichard U.,
RA Renauld H., Robson G.D., Rodriguez de Cordoba S., Rodriguez-Pena J.M.,
RA Ronning C.M., Rutter S., Salzberg S.L., Sanchez M., Sanchez-Ferrero J.C.,
RA Saunders D., Seeger K., Squares R., Squares S., Takeuchi M., Tekaia F.,
RA Turner G., Vazquez de Aldana C.R., Weidman J., White O., Woodward J.R.,
RA Yu J.-H., Fraser C.M., Galagan J.E., Asai K., Machida M., Hall N.,
RA Barrell B.G., Denning D.W.;
RT "Genomic sequence of the pathogenic and allergenic filamentous fungus
RT Aspergillus fumigatus.";
RL Nature 438:1151-1156(2005).
CC -!- FUNCTION: Essential component of the TIM23 complex, a complex that
CC mediates the translocation of transit peptide-containing proteins
CC across the mitochondrial inner membrane. Required to direct preproteins
CC in transit and direct them to the channel protein TIM23, and possibly
CC facilitates transfer of the translocating proteins from the TOM complex
CC to the TIM23 complex (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of the TIM23 complex, at least composed of
CC TIM23/timX, TIM17/timQ, tim50 and TIM21/timU. Interacts with
CC preproteins in transit (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000250};
CC Single-pass membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the TIM50 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=EAL87439.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AAHF01000008; EAL87439.1; ALT_INIT; Genomic_DNA.
DR RefSeq; XP_749477.1; XM_744384.1.
DR AlphaFoldDB; Q4WI16; -.
DR SMR; Q4WI16; -.
DR STRING; 746128.CADAFUBP00002002; -.
DR PRIDE; Q4WI16; -.
DR GeneID; 3507050; -.
DR KEGG; afm:AFUA_2G03420; -.
DR eggNOG; KOG2832; Eukaryota.
DR HOGENOM; CLU_023309_0_0_1; -.
DR InParanoid; Q4WI16; -.
DR OrthoDB; 1142452at2759; -.
DR Proteomes; UP000002530; Chromosome 2.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005744; C:TIM23 mitochondrial import inner membrane translocase complex; IBA:GO_Central.
DR GO; GO:0030150; P:protein import into mitochondrial matrix; IBA:GO_Central.
DR Gene3D; 3.40.50.1000; -; 1.
DR InterPro; IPR004274; FCP1_dom.
DR InterPro; IPR036412; HAD-like_sf.
DR InterPro; IPR023214; HAD_sf.
DR InterPro; IPR027111; Tim50.
DR PANTHER; PTHR12210:SF3; PTHR12210:SF3; 1.
DR Pfam; PF03031; NIF; 1.
DR SMART; SM00577; CPDc; 1.
DR SUPFAM; SSF56784; SSF56784; 1.
DR PROSITE; PS50969; FCP1; 1.
PE 3: Inferred from homology;
KW Membrane; Mitochondrion; Mitochondrion inner membrane; Protein transport;
KW Reference proteome; Transit peptide; Translocation; Transmembrane;
KW Transmembrane helix; Transport.
FT TRANSIT 1..16
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN 17..501
FT /note="Mitochondrial import inner membrane translocase
FT subunit tim50"
FT /id="PRO_0000043126"
FT TOPO_DOM 17..146
FT /note="Mitochondrial matrix"
FT /evidence="ECO:0000255"
FT TRANSMEM 147..164
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 165..501
FT /note="Mitochondrial intermembrane"
FT /evidence="ECO:0000255"
FT DOMAIN 218..361
FT /note="FCP1 homology"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00336"
FT REGION 1..125
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 423..442
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 34..50
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 71..86
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 100..114
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 501 AA; 57307 MW; 55FB08E87010061C CRC64;
MDSAARPVSF PKSRWYAKSS KPKAPYKLPE SVKPPQSEQP SSTPKPEYSA EQTEFDTKAD
PAGNAAPEAS EATESKPQQP LPDLTQGIPS TLAAELEGRT KKSGQTSLNL TEDPSRFEDY
TDDGRGDIPK DGYVSSLDRR RARMANLMYA FFLLAGAGGV AYLGRNWETE EEAKAHPDIP
SGWSFSSWYN RMKARLSDIT SYYKDPAFPK LLPDEDPNLR QPYTLVLSLE DLLVHSEWSR
EHGWRIAKRP GVDYFLRYLN QYYELVLFTS VPSMMADQVL RKLDPYRIIR WPLFREATRY
KDGEYIKDLS YLNRDLSKVI LIDTKEEHAR LQPENAIILD KWLGDPKDKN LVALIPFLEY
IAGMGVEDVR PVLKSFEGTS IPVEFAKRER IMREKFEKEL EEERKRRPKR GVGSLASALG
LKSTRTLDGE QSPSEGLAQG KMLWDQIRER GQKNYEMIEK EIRENGEKWL AEMAAEEEKA
RQEQMKMMKG SFTSMFGAGK Q
