ID   TIM50_BOVIN             Reviewed;         355 AA.
AC   Q3SZB3;
DT   06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   11-OCT-2005, sequence version 1.
DT   25-MAY-2022, entry version 76.
DE   RecName: Full=Mitochondrial import inner membrane translocase subunit TIM50;
DE   Flags: Precursor;
GN   Name=TIMM50; Synonyms=TIM50;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Hereford; TISSUE=Rumen;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Essential component of the TIM23 complex, a complex that
CC       mediates the translocation of transit peptide-containing proteins
CC       across the mitochondrial inner membrane. Has some phosphatase activity
CC       in vitro; however such activity may not be relevant in vivo.
CC       {ECO:0000250|UniProtKB:Q3ZCQ8}.
CC   -!- SUBUNIT: Component of the TIM23 complex at least composed of TIMM23,
CC       TIMM17 (TIMM17A or TIMM17B) and TIMM50; within this complex, directly
CC       interacts with TIMM23. The complex interacts with the TIMM44 component
CC       of the PAM complex and with DNAJC15. {ECO:0000250|UniProtKB:Q3ZCQ8}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC       {ECO:0000250|UniProtKB:Q3ZCQ8}; Single-pass membrane protein
CC       {ECO:0000250|UniProtKB:Q3ZCQ8}.
CC   -!- SIMILARITY: Belongs to the TIM50 family. {ECO:0000305}.
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DR   EMBL; BC102990; AAI02991.1; -; mRNA.
DR   RefSeq; NP_001030196.1; NM_001035024.1.
DR   AlphaFoldDB; Q3SZB3; -.
DR   SMR; Q3SZB3; -.
DR   STRING; 9913.ENSBTAP00000031959; -.
DR   PaxDb; Q3SZB3; -.
DR   PeptideAtlas; Q3SZB3; -.
DR   PRIDE; Q3SZB3; -.
DR   GeneID; 505489; -.
DR   KEGG; bta:505489; -.
DR   CTD; 92609; -.
DR   eggNOG; KOG2832; Eukaryota.
DR   InParanoid; Q3SZB3; -.
DR   OrthoDB; 1142452at2759; -.
DR   Proteomes; UP000009136; Unplaced.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005744; C:TIM23 mitochondrial import inner membrane translocase complex; IEA:InterPro.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.1000; -; 1.
DR   InterPro; IPR004274; FCP1_dom.
DR   InterPro; IPR036412; HAD-like_sf.
DR   InterPro; IPR023214; HAD_sf.
DR   InterPro; IPR027111; Tim50.
DR   PANTHER; PTHR12210:SF3; PTHR12210:SF3; 1.
DR   Pfam; PF03031; NIF; 1.
DR   SMART; SM00577; CPDc; 1.
DR   SUPFAM; SSF56784; SSF56784; 1.
DR   PROSITE; PS50969; FCP1; 1.
PE   2: Evidence at transcript level;
KW   Membrane; Mitochondrion; Mitochondrion inner membrane; Phosphoprotein;
KW   Protein transport; Reference proteome; Transit peptide; Translocation;
KW   Transmembrane; Transmembrane helix; Transport.
FT   TRANSIT         1..44
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           45..355
FT                   /note="Mitochondrial import inner membrane translocase
FT                   subunit TIM50"
FT                   /id="PRO_0000043114"
FT   TOPO_DOM        45..67
FT                   /note="Mitochondrial matrix"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        68..88
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        89..355
FT                   /note="Mitochondrial intermembrane"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          145..288
FT                   /note="FCP1 homology"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00336"
FT   REGION          25..61
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        43..61
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         343
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q3ZCQ8"
SQ   SEQUENCE   355 AA;  40109 MW;  433FB15D8DEBB1AF CRC64;
     MAASAAVFLR LRSGLRQGAR GLCARLATPP PRAPDQAAEI GSRAGTKAQT QGPQQQRSSE
     GPSYAKKVAL WLARLLGAGG TVSVIYIFGN NAVDENGAKI PDEFDNDPIL VQQLRRTYKY
     FKDYRQMIIE PTSPCLLPDP LREPYYQPPY TLVLELTGVL LHPEWSLATG WRFKKRPGIE
     TLFQQLAPLY EIVIFTSETG MTAFPLIDSV DPHGFISYRL FRDATRYMDG HHVKDISCLN
     RDPARVVVVD CKKEAFRLQP YNGVALRPWD GNSDDRVLLD LSAFLKTIAL NGVEDVRTVL
     EHYALEEDPL EAFKQRQSRL EQEEQQRLAE LSKSSKQNLF FSSLTSRLWP RSKQP