ID   TIM50_CAEBR             Reviewed;         456 AA.
AC   Q61JS7; A8X8J6;
DT   06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   23-NOV-2004, sequence version 1.
DT   03-AUG-2022, entry version 86.
DE   RecName: Full=Mitochondrial import inner membrane translocase subunit TIM50;
DE   AltName: Full=Small C-terminal domain Phosphatase protein 4;
DE   Flags: Precursor;
GN   Name=scpl-4; Synonyms=tim-50; ORFNames=CBG09654;
OS   Caenorhabditis briggsae.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6238;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AF16;
RX   PubMed=14624247; DOI=10.1371/journal.pbio.0000045;
RA   Stein L.D., Bao Z., Blasiar D., Blumenthal T., Brent M.R., Chen N.,
RA   Chinwalla A., Clarke L., Clee C., Coghlan A., Coulson A., D'Eustachio P.,
RA   Fitch D.H.A., Fulton L.A., Fulton R.E., Griffiths-Jones S., Harris T.W.,
RA   Hillier L.W., Kamath R., Kuwabara P.E., Mardis E.R., Marra M.A.,
RA   Miner T.L., Minx P., Mullikin J.C., Plumb R.W., Rogers J., Schein J.E.,
RA   Sohrmann M., Spieth J., Stajich J.E., Wei C., Willey D., Wilson R.K.,
RA   Durbin R.M., Waterston R.H.;
RT   "The genome sequence of Caenorhabditis briggsae: a platform for comparative
RT   genomics.";
RL   PLoS Biol. 1:166-192(2003).
CC   -!- FUNCTION: Essential component of the TIM23 complex, a complex that
CC       mediates the translocation of transit peptide-containing proteins
CC       across the mitochondrial inner membrane. {ECO:0000250}.
CC   -!- SUBUNIT: Component of the TIM23 complex at least composed of tim-23,
CC       tim-17 and tim-50. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000250};
CC       Single-pass membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the TIM50 family. {ECO:0000305}.
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DR   EMBL; HE600998; CAP28957.3; -; Genomic_DNA.
DR   RefSeq; XP_002637152.1; XM_002637106.1.
DR   AlphaFoldDB; Q61JS7; -.
DR   SMR; Q61JS7; -.
DR   STRING; 6238.CBG09654; -.
DR   PRIDE; Q61JS7; -.
DR   GeneID; 8579148; -.
DR   KEGG; cbr:CBG_09654; -.
DR   CTD; 8579148; -.
DR   WormBase; CBG09654; CBP45117; WBGene00031212; Cbr-scpl-4.
DR   eggNOG; KOG2832; Eukaryota.
DR   HOGENOM; CLU_685581_0_0_1; -.
DR   InParanoid; Q61JS7; -.
DR   OMA; RMKLGFA; -.
DR   OrthoDB; 707884at2759; -.
DR   Proteomes; UP000008549; Chromosome V.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005744; C:TIM23 mitochondrial import inner membrane translocase complex; IBA:GO_Central.
DR   GO; GO:0030150; P:protein import into mitochondrial matrix; IBA:GO_Central.
DR   Gene3D; 3.40.50.1000; -; 1.
DR   InterPro; IPR004274; FCP1_dom.
DR   InterPro; IPR036412; HAD-like_sf.
DR   InterPro; IPR023214; HAD_sf.
DR   InterPro; IPR027111; Tim50.
DR   PANTHER; PTHR12210:SF3; PTHR12210:SF3; 1.
DR   Pfam; PF03031; NIF; 1.
DR   SMART; SM00577; CPDc; 1.
DR   SUPFAM; SSF56784; SSF56784; 1.
DR   PROSITE; PS50969; FCP1; 1.
PE   3: Inferred from homology;
KW   Membrane; Mitochondrion; Mitochondrion inner membrane; Protein transport;
KW   Reference proteome; Transit peptide; Translocation; Transmembrane;
KW   Transmembrane helix; Transport.
FT   TRANSIT         1..22
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           23..456
FT                   /note="Mitochondrial import inner membrane translocase
FT                   subunit TIM50"
FT                   /id="PRO_0000043119"
FT   TOPO_DOM        23..171
FT                   /note="Mitochondrial matrix"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        172..192
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        193..456
FT                   /note="Mitochondrial intermembrane"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          247..391
FT                   /note="FCP1 homology"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00336"
FT   REGION          101..120
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          132..165
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        133..153
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   456 AA;  51697 MW;  E4D42372F9F38D26 CRC64;
     MSLSKLSQKC FSRHHARTFI RFSSSDFQSL LGPPRVPNPY ADSGRNKFAA PIVPINVNNH
     GNVFASIKVP INETPEAVAF KPEVEEAPRV EKVEVEATKI ETEKVASSPA PATPPSAIDE
     LNSLKDSLSK LEEAAASKTS APSGSSGDNN DQPGNAEEVE ARRKRMERNT RIGGYVLLGG
     SVIGFISFCF YYGRAQRDEA GNVIADEFSG SFLAPFYRIA NSFKLWKDYV VEPAREQLLP
     DPLPAPYLQP KYTIVIELKN ILVHPEWTYK TGYRFLKRPA LDYFLDVIGY PNFEVVIYSS
     ESMMTAAPVV DSFDQKQRIM YKLFRDCTKY MNGHHVKDLS KLNRDLSKVI YIDFDAKSGQ
     LNPENMLRVP EWRGNMDDTS LVDLAELLKT IHLSDAEDVR PMLQYYSQYD DPAKEFRRRA
     VYLAQQEEQK KQQPDDSSML KRYSGRLFGF RRHASA