ID   TIM50_CAEEL             Reviewed;         452 AA.
AC   Q22647;
DT   06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 124.
DE   RecName: Full=Mitochondrial import inner membrane translocase subunit TIM50;
DE   AltName: Full=Small C-terminal domain Phosphatase protein 4;
DE   Flags: Precursor;
GN   Name=scpl-4; Synonyms=tim-50; ORFNames=T21C9.12;
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6239;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2;
RX   PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for investigating
RT   biology.";
RL   Science 282:2012-2018(1998).
CC   -!- FUNCTION: Essential component of the TIM23 complex, a complex that
CC       mediates the translocation of transit peptide-containing proteins
CC       across the mitochondrial inner membrane. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000250};
CC       Single-pass membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the TIM50 family. {ECO:0000305}.
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DR   EMBL; Z73098; CAA97339.1; -; Genomic_DNA.
DR   PIR; T25076; T25076.
DR   RefSeq; NP_505722.1; NM_073321.5.
DR   AlphaFoldDB; Q22647; -.
DR   SMR; Q22647; -.
DR   BioGRID; 44510; 4.
DR   STRING; 6239.T21C9.12; -.
DR   EPD; Q22647; -.
DR   PaxDb; Q22647; -.
DR   PeptideAtlas; Q22647; -.
DR   EnsemblMetazoa; T21C9.12a.1; T21C9.12a.1; WBGene00011897.
DR   GeneID; 179481; -.
DR   KEGG; cel:CELE_T21C9.12; -.
DR   UCSC; T21C9.12; c. elegans.
DR   CTD; 179481; -.
DR   WormBase; T21C9.12a; CE06481; WBGene00011897; scpl-4.
DR   eggNOG; KOG2832; Eukaryota.
DR   GeneTree; ENSGT01040000240503; -.
DR   HOGENOM; CLU_685581_0_0_1; -.
DR   InParanoid; Q22647; -.
DR   OMA; RMKLGFA; -.
DR   OrthoDB; 707884at2759; -.
DR   PhylomeDB; Q22647; -.
DR   PRO; PR:Q22647; -.
DR   Proteomes; UP000001940; Chromosome V.
DR   Bgee; WBGene00011897; Expressed in germ line (C elegans) and 4 other tissues.
DR   ExpressionAtlas; Q22647; baseline and differential.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005744; C:TIM23 mitochondrial import inner membrane translocase complex; IBA:GO_Central.
DR   GO; GO:0030150; P:protein import into mitochondrial matrix; IBA:GO_Central.
DR   Gene3D; 3.40.50.1000; -; 1.
DR   InterPro; IPR004274; FCP1_dom.
DR   InterPro; IPR036412; HAD-like_sf.
DR   InterPro; IPR023214; HAD_sf.
DR   InterPro; IPR027111; Tim50.
DR   PANTHER; PTHR12210:SF3; PTHR12210:SF3; 1.
DR   Pfam; PF03031; NIF; 1.
DR   SMART; SM00577; CPDc; 1.
DR   SUPFAM; SSF56784; SSF56784; 1.
DR   PROSITE; PS50969; FCP1; 1.
PE   3: Inferred from homology;
KW   Membrane; Mitochondrion; Mitochondrion inner membrane; Protein transport;
KW   Reference proteome; Transit peptide; Translocation; Transmembrane;
KW   Transmembrane helix; Transport.
FT   TRANSIT         1..23
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           24..452
FT                   /note="Mitochondrial import inner membrane translocase
FT                   subunit TIM50"
FT                   /id="PRO_0000043120"
FT   TOPO_DOM        24..167
FT                   /note="Mitochondrial matrix"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        168..188
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        189..452
FT                   /note="Mitochondrial intermembrane"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          243..387
FT                   /note="FCP1 homology"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00336"
FT   REGION          96..115
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          130..153
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        131..150
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   452 AA;  51378 MW;  291C1F6A4E99A95A CRC64;
     MSLSKLTQTC FSRHQAKTFI RLYSSDFKSL LGPPAVANPY ADNGRTRFAP IVPINHGNVF
     ASIKLPINET QEAIAFKSEV EEAPKVEKLE VESPKIEAEK VLSSPPPAPA PTSSAIDELN
     SLKDSLEKLE SAASKSSSSS GGSSDNSDPG NAEEIEARRK RMERNTRIGA YVLFGGSIIG
     FISFCFYYGR AQRDEFGNVI SDEFSGSFLA PFYRIANSFK LWRDYVVEPA REQLLPDPLP
     APYLQPKYTI VIELKNILVH PEWTYKTGYR FLKRPALDYF LDVIGYPNFE VVIYSSESMM
     TAAPVVDSFD PKQRIMYKLF RDCTKYMNGH HVKDLSKLNR DLSKVIYIDF DAKSGQLNPE
     NMLRVPEWKG NMDDTSLVDL AELLKTIHLS DAEDVRPMLQ YYSQYDDPAK EFRRRAVYLS
     QQEEQKKQQP DDSSMLKRYS GRLFGSRRHV NA