ID   TIM50_CANAL             Reviewed;         469 AA.
AC   Q59W44; A0A1D8PF38;
DT   06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   26-APR-2005, sequence version 1.
DT   03-AUG-2022, entry version 92.
DE   RecName: Full=Mitochondrial import inner membrane translocase subunit TIM50;
DE   Flags: Precursor;
GN   Name=TIM50; OrderedLocusNames=CAALFM_C111220CA;
GN   ORFNames=CaO19.680, CaO19.8297;
OS   Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade; Candida.
OX   NCBI_TaxID=237561;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SC5314 / ATCC MYA-2876;
RX   PubMed=15123810; DOI=10.1073/pnas.0401648101;
RA   Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B.,
RA   Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W.,
RA   Scherer S.;
RT   "The diploid genome sequence of Candida albicans.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=SC5314 / ATCC MYA-2876;
RX   PubMed=17419877; DOI=10.1186/gb-2007-8-4-r52;
RA   van het Hoog M., Rast T.J., Martchenko M., Grindle S., Dignard D.,
RA   Hogues H., Cuomo C., Berriman M., Scherer S., Magee B.B., Whiteway M.,
RA   Chibana H., Nantel A., Magee P.T.;
RT   "Assembly of the Candida albicans genome into sixteen supercontigs aligned
RT   on the eight chromosomes.";
RL   Genome Biol. 8:RESEARCH52.1-RESEARCH52.12(2007).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC   STRAIN=SC5314 / ATCC MYA-2876;
RX   PubMed=24025428; DOI=10.1186/gb-2013-14-9-r97;
RA   Muzzey D., Schwartz K., Weissman J.S., Sherlock G.;
RT   "Assembly of a phased diploid Candida albicans genome facilitates allele-
RT   specific measurements and provides a simple model for repeat and indel
RT   structure.";
RL   Genome Biol. 14:RESEARCH97.1-RESEARCH97.14(2013).
CC   -!- FUNCTION: Essential component of the TIM23 complex, a complex that
CC       mediates the translocation of transit peptide-containing proteins
CC       across the mitochondrial inner membrane. Required to direct preproteins
CC       in transit and direct them to the channel protein TIM23, and possibly
CC       facilitates transfer of the translocating proteins from the TOM complex
CC       to the TIM23 complex (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the TIM23 complex, at least composed of TIM23,
CC       TIM17, TIM50 and TIM21. Interacts with preproteins in transit (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000250};
CC       Single-pass membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the TIM50 family. {ECO:0000305}.
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DR   EMBL; CP017623; AOW26747.1; -; Genomic_DNA.
DR   RefSeq; XP_713813.1; XM_708720.1.
DR   AlphaFoldDB; Q59W44; -.
DR   SMR; Q59W44; -.
DR   STRING; 237561.Q59W44; -.
DR   PRIDE; Q59W44; -.
DR   GeneID; 3644530; -.
DR   KEGG; cal:CAALFM_C111220CA; -.
DR   CGD; CAL0000183476; TIM50.
DR   VEuPathDB; FungiDB:C1_11220C_A; -.
DR   eggNOG; KOG2832; Eukaryota.
DR   HOGENOM; CLU_023309_1_2_1; -.
DR   InParanoid; Q59W44; -.
DR   OrthoDB; 1142452at2759; -.
DR   PRO; PR:Q59W44; -.
DR   Proteomes; UP000000559; Chromosome 1.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IDA:CGD.
DR   GO; GO:0005744; C:TIM23 mitochondrial import inner membrane translocase complex; IBA:GO_Central.
DR   GO; GO:0030150; P:protein import into mitochondrial matrix; IBA:GO_Central.
DR   Gene3D; 3.40.50.1000; -; 1.
DR   InterPro; IPR004274; FCP1_dom.
DR   InterPro; IPR036412; HAD-like_sf.
DR   InterPro; IPR023214; HAD_sf.
DR   InterPro; IPR027111; Tim50.
DR   PANTHER; PTHR12210:SF3; PTHR12210:SF3; 1.
DR   Pfam; PF03031; NIF; 1.
DR   SMART; SM00577; CPDc; 1.
DR   SUPFAM; SSF56784; SSF56784; 1.
DR   PROSITE; PS50969; FCP1; 1.
PE   3: Inferred from homology;
KW   Membrane; Mitochondrion; Mitochondrion inner membrane; Protein transport;
KW   Reference proteome; Transit peptide; Translocation; Transmembrane;
KW   Transmembrane helix; Transport.
FT   TRANSIT         1..16
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           17..469
FT                   /note="Mitochondrial import inner membrane translocase
FT                   subunit TIM50"
FT                   /id="PRO_0000043127"
FT   TOPO_DOM        17..108
FT                   /note="Mitochondrial matrix"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        109..125
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        126..469
FT                   /note="Mitochondrial intermembrane"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          183..326
FT                   /note="FCP1 homology"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00336"
FT   REGION          70..99
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   469 AA;  54241 MW;  BE6AE66F1554C3BF CRC64;
     MFRTQSRYLV RSILANHCVL PSTRMAVSPI LNQSIKFYSS KDNATDKSQE KEQPKSILND
     DMLARAGFED VDPKEETSNS EEQATKQTGR KRKRAQTSKD LQRERYANMF YLSALIFGVA
     GVGYMSRDWD SEKEQEEMDG KNVENGYTPK LMYERLSKRL GSLFTFFSEP AFENLLPPPP
     PEQYRRPLTL VVTLDDFLIH SNWDTQHGWR TGKRPGLDYF LGYLSQYYEI VVFSSNSQIY
     SDKTVNKLDP YHAYISYALF REACRYKDGK LIKDLSLLNR DLGKTVMIDV DEDSAALQPE
     NSIIVKKWEG QPDEYLISLI PFLEYLATQP VKDVRPILNS YKDKSNIVAE FAERENKLRE
     QWRKDHGGNN GKPNAGNFIA KLLGIPVPEP KMPLDIIREH GQLQYEHFQK YLQENAHKFL
     EEEQKLKDEF GKVTLNKLIT EGAPNAEEIA KVQQQRALEE AQKQQEGHK