ID   TIM50_DICDI             Reviewed;         374 AA.
AC   Q55C70;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   25-MAY-2022, entry version 76.
DE   RecName: Full=Mitochondrial import inner membrane translocase subunit tim50;
DE   Flags: Precursor;
GN   Name=timm50; ORFNames=DDB_G0270196;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- FUNCTION: Component of the mitochondrial import inner membrane
CC       translocase that mediates the translocation of transit peptide-
CC       containing proteins across the mitochondrial inner membrane.
CC       {ECO:0000250|UniProtKB:Q3ZCQ8}.
CC   -!- SUBUNIT: Component of the mitochondrial import inner membrane
CC       translocase complex. {ECO:0000250|UniProtKB:Q3ZCQ8}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC       {ECO:0000250|UniProtKB:Q3ZCQ8}; Single-pass membrane protein
CC       {ECO:0000250|UniProtKB:Q3ZCQ8}.
CC   -!- SIMILARITY: Belongs to the TIM50 family. {ECO:0000305}.
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DR   EMBL; AAFI02000005; EAL72448.1; -; Genomic_DNA.
DR   RefSeq; XP_646611.1; XM_641519.1.
DR   AlphaFoldDB; Q55C70; -.
DR   SMR; Q55C70; -.
DR   STRING; 44689.DDB0304712; -.
DR   PaxDb; Q55C70; -.
DR   EnsemblProtists; EAL72448; EAL72448; DDB_G0270196.
DR   GeneID; 8617583; -.
DR   KEGG; ddi:DDB_G0270196; -.
DR   dictyBase; DDB_G0270196; -.
DR   eggNOG; KOG2832; Eukaryota.
DR   HOGENOM; CLU_740641_0_0_1; -.
DR   InParanoid; Q55C70; -.
DR   OMA; RNQFSKK; -.
DR   PhylomeDB; Q55C70; -.
DR   PRO; PR:Q55C70; -.
DR   Proteomes; UP000002195; Chromosome 1.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005744; C:TIM23 mitochondrial import inner membrane translocase complex; IBA:GO_Central.
DR   GO; GO:0030150; P:protein import into mitochondrial matrix; IBA:GO_Central.
DR   Gene3D; 3.40.50.1000; -; 1.
DR   InterPro; IPR004274; FCP1_dom.
DR   InterPro; IPR036412; HAD-like_sf.
DR   InterPro; IPR023214; HAD_sf.
DR   InterPro; IPR027111; Tim50.
DR   PANTHER; PTHR12210:SF3; PTHR12210:SF3; 1.
DR   Pfam; PF03031; NIF; 1.
DR   SMART; SM00577; CPDc; 1.
DR   SUPFAM; SSF56784; SSF56784; 1.
PE   3: Inferred from homology;
KW   Membrane; Mitochondrion; Mitochondrion inner membrane; Protein transport;
KW   Reference proteome; Transit peptide; Translocation; Transmembrane;
KW   Transmembrane helix; Transport.
FT   TRANSIT         1..48
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           49..374
FT                   /note="Mitochondrial import inner membrane translocase
FT                   subunit tim50"
FT                   /id="PRO_0000367257"
FT   TRANSMEM        103..125
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          191..332
FT                   /note="FCP1 homology"
FT   REGION          42..97
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        50..78
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   374 AA;  43667 MW;  0F495F262426B811 CRC64;
     MILNKVAKCY GKQIGFFGNK TTQFIKPNQT IFLIGGTKRL FTTQQQQSPK KEEPKSEQQK
     KVEDKTEEKE KEKDEEENEN EKEKENEDGE GQKKKSKFNV PPIVTSVTST FFAGVLVAST
     FGYLTYNFKK DISEEERYRL NSVESKFYHS IAEPFREFFD NIFENLRTKY EFFDMLFGPG
     KIHKVLPPPL PGGKKYTLVI DIDALTEITK TSKYPTLYKR AGLDFFLDHL RKDYEIYLYF
     NGNIPQNKYE QLQFKIDTNG KYFTGLLYPE TGIKERNQFS KKIEMLDRDP SKVIFIDAAS
     PYDHPNVINI GKFKSNSKDK LLIELLPVLE SFSRKNLDDV RPEISQFQNI SKQSLTKNLE
     DYLSTHNINS RQKK