ID   TIM50_NEUCR             Reviewed;         540 AA.
AC   Q874C1; Q7SHG1;
DT   06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   25-MAY-2022, entry version 99.
DE   RecName: Full=Mitochondrial import inner membrane translocase subunit tim50;
DE   Flags: Precursor;
GN   Name=tim50; ORFNames=NCU02943;
OS   Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 /
OS   FGSC 987).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Sordariomycetidae; Sordariales; Sordariaceae; Neurospora.
OX   NCBI_TaxID=367110;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF N-TERMINUS, IDENTIFICATION
RP   BY MASS SPECTROMETRY, IDENTIFICATION IN THE TIM23 COMPLEX, FUNCTION,
RP   SUBCELLULAR LOCATION, AND TOPOLOGY.
RX   PubMed=12574118; DOI=10.1093/emboj/cdg090;
RA   Mokranjac D., Paschen S.A., Kozany C., Prokisch H., Hoppins S.C.,
RA   Nargang F.E., Neupert W., Hell K.;
RT   "Tim50, a novel component of the TIM23 preprotein translocase of
RT   mitochondria.";
RL   EMBO J. 22:816-825(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX   PubMed=12712197; DOI=10.1038/nature01554;
RA   Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D.,
RA   Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B.,
RA   Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M.,
RA   Qui D., Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M.,
RA   Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U.,
RA   Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D.,
RA   Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S.,
RA   Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D.,
RA   Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S.,
RA   Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A.,
RA   DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R.,
RA   Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R.,
RA   Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I.,
RA   Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.;
RT   "The genome sequence of the filamentous fungus Neurospora crassa.";
RL   Nature 422:859-868(2003).
CC   -!- FUNCTION: Essential component of the TIM23 complex, a complex that
CC       mediates the translocation of transit peptide-containing proteins
CC       across the mitochondrial inner membrane. Required to direct preproteins
CC       in transit and direct them to the channel protein tim23, and possibly
CC       facilitates transfer of the translocating proteins from the TOM complex
CC       to the TIM23 complex. {ECO:0000269|PubMed:12574118}.
CC   -!- SUBUNIT: Component of the TIM23 complex, at least composed of tim23,
CC       tim17, tim50 and tim21 (Probable). Interacts with preproteins in
CC       transit (By similarity). {ECO:0000250, ECO:0000305}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC       {ECO:0000269|PubMed:12574118}; Single-pass membrane protein
CC       {ECO:0000269|PubMed:12574118}.
CC   -!- SIMILARITY: Belongs to the TIM50 family. {ECO:0000305}.
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DR   EMBL; AY188754; AAO32939.1; -; mRNA.
DR   EMBL; CM002236; EAA36389.3; -; Genomic_DNA.
DR   RefSeq; XP_965625.3; XM_960532.3.
DR   AlphaFoldDB; Q874C1; -.
DR   SMR; Q874C1; -.
DR   STRING; 5141.EFNCRP00000002384; -.
DR   EnsemblFungi; EAA36389; EAA36389; NCU02943.
DR   GeneID; 3881750; -.
DR   KEGG; ncr:NCU02943; -.
DR   VEuPathDB; FungiDB:NCU02943; -.
DR   HOGENOM; CLU_023309_0_0_1; -.
DR   InParanoid; Q874C1; -.
DR   Proteomes; UP000001805; Chromosome 1, Linkage Group I.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005744; C:TIM23 mitochondrial import inner membrane translocase complex; IBA:GO_Central.
DR   GO; GO:0030150; P:protein import into mitochondrial matrix; IBA:GO_Central.
DR   Gene3D; 3.40.50.1000; -; 1.
DR   InterPro; IPR004274; FCP1_dom.
DR   InterPro; IPR036412; HAD-like_sf.
DR   InterPro; IPR023214; HAD_sf.
DR   InterPro; IPR027111; Tim50.
DR   PANTHER; PTHR12210:SF3; PTHR12210:SF3; 1.
DR   Pfam; PF03031; NIF; 1.
DR   SMART; SM00577; CPDc; 1.
DR   SUPFAM; SSF56784; SSF56784; 1.
DR   PROSITE; PS50969; FCP1; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Membrane; Mitochondrion;
KW   Mitochondrion inner membrane; Protein transport; Reference proteome;
KW   Transit peptide; Translocation; Transmembrane; Transmembrane helix;
KW   Transport.
FT   TRANSIT         1..38
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000269|PubMed:12574118"
FT   CHAIN           39..540
FT                   /note="Mitochondrial import inner membrane translocase
FT                   subunit tim50"
FT                   /id="PRO_0000043134"
FT   TOPO_DOM        39..171
FT                   /note="Mitochondrial matrix"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        172..191
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        192..540
FT                   /note="Mitochondrial intermembrane"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          244..387
FT                   /note="FCP1 homology"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00336"
FT   REGION          35..164
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          519..540
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        72..87
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   540 AA;  60593 MW;  CDCC8BBAFC2A8E2C CRC64;
     MMLSRAAVRS IAGARVAAHA ASPLLSQRTL PAVWTRSMAK DNKPPKFSKP ESTPAQKATP
     KAPEPAESEK AEQKQQQQQQ QQTPAESEPE PEIDLSKLPD LRGGIPTTLE YEMAQKEAGK
     KPVAGEEAET QAEGAEGPEA ATSGSGGGGR KKGQLPDSAY VSSTEKRRQK MANWAFIAAG
     LALVGGTIYL GREWDEEELE KHHDIPNGWG LGLWWKRAKA RMTGTVSYYQ EPAFEKLLPD
     PDPSFERPYT LCISLEDMLV HSEWTRDHGW RLAKRPGVDY FLRYLSQYYE IVLFTSVPFA
     NAEPIVRKMD PYRFIMWPLF REATKYKDGE IVKDLSYLNR DLSKVIIIDT DPKHVRAQPE
     NAIVLPKWKG DPKDTELVSL VPFLEFIHTM NFPDVRKVLK SFEGQHIPTE FARREAIARA
     EHNKLVAAKA KKAGLGSLGA RFGIKPSKLN PMAMEGEEDP SEAFAKGKMI QDIARERGMR
     NYLAMEEEIK KNGEMWLKME QEAQEKAQKE MMKNMQSSVF GWFGGAPSGE QQSGESEKKA