ID   TIM54_ASHGO             Reviewed;         472 AA.
AC   Q758C9;
DT   21-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2013, sequence version 2.
DT   25-MAY-2022, entry version 92.
DE   RecName: Full=Mitochondrial import inner membrane translocase subunit TIM54;
GN   Name=TIM54; OrderedLocusNames=AEL177C;
OS   Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
OS   (Yeast) (Eremothecium gossypii).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Eremothecium.
OX   NCBI_TaxID=284811;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=15001715; DOI=10.1126/science.1095781;
RA   Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S.,
RA   Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A.,
RA   Gaffney T.D., Philippsen P.;
RT   "The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces
RT   cerevisiae genome.";
RL   Science 304:304-307(2004).
RN   [2]
RP   GENOME REANNOTATION, AND SEQUENCE REVISION TO 272; 284; 290; 297; 323 AND
RP   350.
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=23749448; DOI=10.1534/g3.112.002881;
RA   Dietrich F.S., Voegeli S., Kuo S., Philippsen P.;
RT   "Genomes of Ashbya fungi isolated from insects reveal four mating-type
RT   loci, numerous translocations, lack of transposons, and distinct gene
RT   duplications.";
RL   G3 (Bethesda) 3:1225-1239(2013).
CC   -!- FUNCTION: Essential component of the TIM22 complex, a complex that
CC       mediates the import and insertion of multi-pass transmembrane proteins
CC       into the mitochondrial inner membrane. The TIM22 complex forms a twin-
CC       pore translocase that uses the membrane potential as external driving
CC       force (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the TIM22 complex, whose core is composed of
CC       TIM22 and TIM54, associated with the 70 kDa heterohexamer composed of
CC       TIM9 and TIM10 (or TIM8 and TIM13). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000250};
CC       Single-pass membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the TIM54 family. {ECO:0000305}.
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DR   EMBL; AE016818; AAS52508.2; -; Genomic_DNA.
DR   RefSeq; NP_984684.2; NM_210037.2.
DR   AlphaFoldDB; Q758C9; -.
DR   SMR; Q758C9; -.
DR   STRING; 33169.AAS52508; -.
DR   PRIDE; Q758C9; -.
DR   EnsemblFungi; AAS52508; AAS52508; AGOS_AEL177C.
DR   GeneID; 4620869; -.
DR   KEGG; ago:AGOS_AEL177C; -.
DR   eggNOG; ENOG502QPMQ; Eukaryota.
DR   HOGENOM; CLU_039097_0_0_1; -.
DR   InParanoid; Q758C9; -.
DR   OMA; EEKEWHK; -.
DR   Proteomes; UP000000591; Chromosome V.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0042721; C:TIM22 mitochondrial import inner membrane insertion complex; IBA:GO_Central.
DR   GO; GO:0008320; F:protein transmembrane transporter activity; IEA:EnsemblFungi.
DR   GO; GO:0045039; P:protein insertion into mitochondrial inner membrane; IBA:GO_Central.
DR   InterPro; IPR021056; Mt_import_IM_translocase_Tim54.
DR   Pfam; PF11711; Tim54; 1.
PE   3: Inferred from homology;
KW   Membrane; Mitochondrion; Mitochondrion inner membrane; Protein transport;
KW   Reference proteome; Translocation; Transmembrane; Transmembrane helix;
KW   Transport.
FT   CHAIN           1..472
FT                   /note="Mitochondrial import inner membrane translocase
FT                   subunit TIM54"
FT                   /id="PRO_0000228010"
FT   TOPO_DOM        1..37
FT                   /note="Mitochondrial matrix"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        38..58
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        59..472
FT                   /note="Mitochondrial intermembrane"
FT                   /evidence="ECO:0000255"
FT   REGION          1..20
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          276..336
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   472 AA;  53340 MW;  8BB5557CFB61EA00 CRC64;
     MMGEFKHPPQ EKQKMSSTKK AGYSNPAFAA MGIPALRLPG PKWCAFWLVV GAGIAGVVYD
     KREQRRICKH YSSLVKDQGA AHMDTWLKPR RLTVFVAPPP GDYLETSMKV WRRYVKQVLF
     EAGLDYEVFT EERQGVIRHE VAERVRQLRR DIAAAEAAEQ RALDEKRWLN RMRSWWRREK
     LSEEELERIR AQKFRDEFTY KQLLGVFYKN APLREQKVWD ADALVEDPVL AGGVICIGRG
     AYKEYIAGIH EGTLGPLDPP TLTPEAPTEL SAAAEDLTQA PEQPEPSAAA EALKQAPEQA
     EHSPAEPDSA ATDAPDEKSN NAPAPPPAPY VSPDEYSSLS LSQELVGDVR HPSSHIPALF
     HQPLLVIPVP NLSGFLQTPR KIYRFYTRRY YAEECCKAAA AMVLQTIRPF QPDDLNLGIS
     EEEDWPRRWV EQGRERKSEW VQDLKADPRV IAELHVVDPA LMADLAATMN LN