ID   TIM54_ASPOR             Reviewed;         445 AA.
AC   Q2UJY4;
DT   21-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT   24-JAN-2006, sequence version 1.
DT   25-MAY-2022, entry version 69.
DE   RecName: Full=Mitochondrial import inner membrane translocase subunit tim54;
GN   Name=tim54; ORFNames=AO090003001024;
OS   Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Circumdati.
OX   NCBI_TaxID=510516;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 42149 / RIB 40;
RX   PubMed=16372010; DOI=10.1038/nature04300;
RA   Machida M., Asai K., Sano M., Tanaka T., Kumagai T., Terai G., Kusumoto K.,
RA   Arima T., Akita O., Kashiwagi Y., Abe K., Gomi K., Horiuchi H.,
RA   Kitamoto K., Kobayashi T., Takeuchi M., Denning D.W., Galagan J.E.,
RA   Nierman W.C., Yu J., Archer D.B., Bennett J.W., Bhatnagar D.,
RA   Cleveland T.E., Fedorova N.D., Gotoh O., Horikawa H., Hosoyama A.,
RA   Ichinomiya M., Igarashi R., Iwashita K., Juvvadi P.R., Kato M., Kato Y.,
RA   Kin T., Kokubun A., Maeda H., Maeyama N., Maruyama J., Nagasaki H.,
RA   Nakajima T., Oda K., Okada K., Paulsen I., Sakamoto K., Sawano T.,
RA   Takahashi M., Takase K., Terabayashi Y., Wortman J.R., Yamada O.,
RA   Yamagata Y., Anazawa H., Hata Y., Koide Y., Komori T., Koyama Y.,
RA   Minetoki T., Suharnan S., Tanaka A., Isono K., Kuhara S., Ogasawara N.,
RA   Kikuchi H.;
RT   "Genome sequencing and analysis of Aspergillus oryzae.";
RL   Nature 438:1157-1161(2005).
CC   -!- FUNCTION: Essential component of the TIM22 complex, a complex that
CC       mediates the import and insertion of multi-pass transmembrane proteins
CC       into the mitochondrial inner membrane. The TIM22 complex forms a twin-
CC       pore translocase that uses the membrane potential as external driving
CC       force (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the TIM22 complex, whose core is composed of
CC       TIM22 and TIM54, associated with the 70 kDa heterohexamer composed of
CC       TIM9 and TIM10 (or TIM8 and TIM13). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000250};
CC       Single-pass membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the TIM54 family. {ECO:0000305}.
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DR   EMBL; AP007155; BAE58131.1; -; Genomic_DNA.
DR   RefSeq; XP_001820133.2; XM_001820081.2.
DR   AlphaFoldDB; Q2UJY4; -.
DR   SMR; Q2UJY4; -.
DR   STRING; 510516.Q2UJY4; -.
DR   EnsemblFungi; BAE58131; BAE58131; AO090003001024.
DR   GeneID; 5992116; -.
DR   KEGG; aor:AO090003001024; -.
DR   HOGENOM; CLU_039097_1_0_1; -.
DR   Proteomes; UP000006564; Chromosome 2.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   InterPro; IPR021056; Mt_import_IM_translocase_Tim54.
DR   Pfam; PF11711; Tim54; 1.
PE   3: Inferred from homology;
KW   Membrane; Mitochondrion; Mitochondrion inner membrane; Protein transport;
KW   Reference proteome; Translocation; Transmembrane; Transmembrane helix;
KW   Transport.
FT   CHAIN           1..445
FT                   /note="Mitochondrial import inner membrane translocase
FT                   subunit tim54"
FT                   /id="PRO_0000228012"
FT   TOPO_DOM        1..11
FT                   /note="Mitochondrial matrix"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        12..29
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        30..445
FT                   /note="Mitochondrial intermembrane"
FT                   /evidence="ECO:0000255"
FT   REGION          175..252
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        190..207
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        208..227
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        236..252
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   445 AA;  50011 MW;  FDDF0697A0138E67 CRC64;
     MPNFRLKLPS RNWMIFLTVT GSFTAALVYD RKQKKRAQQK WCDLVAHLSK ESLPVDQTRR
     KLTVFLSAPP GDGLRVAREH FKEYVKPILV AAALDYQVIE GRREGEIRAG LAERIRKFRR
     KSGEPSTVVE ETGIEEVVAD AREKIGVVEE PVPKGDLIIG RNTWKEYIRG LHEGWLGPLD
     PPQPPLSTDV PSPSEGAETN GSPDDTPTAE NSEKKEEPEK KDEKPSKPTG PTPAYITPAD
     YSSQSLPRSL PQSLDGSVPI QFPHILGFLN TPIRIYRYLN QRYLADSVGR EVAGIVLAST
     TRPYSDGSFS TDSELTPAGI DGAPASDNLL GGNYEQKTLL EEEEKDWHKS AHKKDEANPD
     KEREWVDSVV LDPRIAARMQ RYVLSPEDEA RSQRIAEGAE YILGEERPTP VPFWQRMWIK
     YGYGEDEETL KRKPIIGNID GEDDQ