ID   TIM54_CANAL             Reviewed;         400 AA.
AC   P48990; A0A1D8PR89; Q3MP44; Q5A5D9; Q5A5K8;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2017, sequence version 3.
DT   25-MAY-2022, entry version 82.
DE   RecName: Full=Mitochondrial import inner membrane translocase subunit TIM54;
GN   Name=TIM54; OrderedLocusNames=CAALFM_C703120WA;
GN   ORFNames=CaJ7.0359, CaO19.12608, CaO19.5143;
OS   Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade; Candida.
OX   NCBI_TaxID=237561;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SC5314 / ATCC MYA-2876;
RX   PubMed=15937140; DOI=10.1534/genetics.104.034652;
RA   Chibana H., Oka N., Nakayama H., Aoyama T., Magee B.B., Magee P.T.,
RA   Mikami Y.;
RT   "Sequence finishing and gene mapping for Candida albicans chromosome 7 and
RT   syntenic analysis against the Saccharomyces cerevisiae genome.";
RL   Genetics 170:1525-1537(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SC5314 / ATCC MYA-2876;
RX   PubMed=15123810; DOI=10.1073/pnas.0401648101;
RA   Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B.,
RA   Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W.,
RA   Scherer S.;
RT   "The diploid genome sequence of Candida albicans.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=SC5314 / ATCC MYA-2876;
RX   PubMed=17419877; DOI=10.1186/gb-2007-8-4-r52;
RA   van het Hoog M., Rast T.J., Martchenko M., Grindle S., Dignard D.,
RA   Hogues H., Cuomo C., Berriman M., Scherer S., Magee B.B., Whiteway M.,
RA   Chibana H., Nantel A., Magee P.T.;
RT   "Assembly of the Candida albicans genome into sixteen supercontigs aligned
RT   on the eight chromosomes.";
RL   Genome Biol. 8:RESEARCH52.1-RESEARCH52.12(2007).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC   STRAIN=SC5314 / ATCC MYA-2876;
RX   PubMed=24025428; DOI=10.1186/gb-2013-14-9-r97;
RA   Muzzey D., Schwartz K., Weissman J.S., Sherlock G.;
RT   "Assembly of a phased diploid Candida albicans genome facilitates allele-
RT   specific measurements and provides a simple model for repeat and indel
RT   structure.";
RL   Genome Biol. 14:RESEARCH97.1-RESEARCH97.14(2013).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 283-400.
RC   STRAIN=10127/5;
RX   PubMed=7916311; DOI=10.1016/0378-1119(94)90049-3;
RA   Daly S., Mastromei G., Yacoub A., Lorenzetti R.;
RT   "Sequence of a dihydrofolate reductase-encoding gene from Candida
RT   albicans.";
RL   Gene 147:115-118(1994).
CC   -!- FUNCTION: Essential component of the TIM22 complex, a complex that
CC       mediates the import and insertion of multi-pass transmembrane proteins
CC       into the mitochondrial inner membrane. The TIM22 complex forms a twin-
CC       pore translocase that uses the membrane potential as external driving
CC       force (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the TIM22 complex, whose core is composed of
CC       TIM22 and TIM54, associated with the 70 kDa heterohexamer composed of
CC       TIM9 and TIM10 (or TIM8 and TIM13). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000250};
CC       Single-pass membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the TIM54 family. {ECO:0000305}.
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DR   EMBL; AP006852; BAE44816.1; -; Genomic_DNA.
DR   EMBL; CP017629; AOW30657.1; -; Genomic_DNA.
DR   EMBL; X78968; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   RefSeq; XP_716966.2; XM_711873.2.
DR   AlphaFoldDB; P48990; -.
DR   SMR; P48990; -.
DR   STRING; 237561.P48990; -.
DR   GeneID; 3641349; -.
DR   KEGG; cal:CAALFM_C703120WA; -.
DR   CGD; CAL0000174120; TIM54.
DR   VEuPathDB; FungiDB:C7_03120W_A; -.
DR   eggNOG; ENOG502QPMQ; Eukaryota.
DR   HOGENOM; CLU_039097_0_0_1; -.
DR   InParanoid; P48990; -.
DR   OrthoDB; 960376at2759; -.
DR   Proteomes; UP000000559; Chromosome 7.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0042721; C:TIM22 mitochondrial import inner membrane insertion complex; IBA:GO_Central.
DR   GO; GO:0045039; P:protein insertion into mitochondrial inner membrane; IBA:GO_Central.
DR   InterPro; IPR021056; Mt_import_IM_translocase_Tim54.
DR   Pfam; PF11711; Tim54; 1.
PE   3: Inferred from homology;
KW   Membrane; Mitochondrion; Mitochondrion inner membrane; Protein transport;
KW   Reference proteome; Translocation; Transmembrane; Transmembrane helix;
KW   Transport.
FT   CHAIN           1..400
FT                   /note="Mitochondrial import inner membrane translocase
FT                   subunit TIM54"
FT                   /id="PRO_0000203063"
FT   TOPO_DOM        1..32
FT                   /note="Mitochondrial matrix"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        33..49
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        50..400
FT                   /note="Mitochondrial intermembrane"
FT                   /evidence="ECO:0000255"
FT   REGION          143..166
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          240..266
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        273
FT                   /note="K -> Q (in Ref. 1; BAE44816)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        283..284
FT                   /note="AP -> EF (in Ref. 5; X78968)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        287
FT                   /note="D -> N (in Ref. 5; X78968)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        333
FT                   /note="L -> I (in Ref. 5; X78968)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        365
FT                   /note="M -> I (in Ref. 5; X78968)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   400 AA;  46113 MW;  B41D82C7373EBAD9 CRC64;
     MPDANEVKPK KGWSNPALRM MGIPRISLPS RNWMIFWTVV TTIGGGIAYD KYEQKQMRKK
     WMDAVKQFGE VSYGANEIPR KLSIFIAPPP NDFLDESLKL FRKFIKPVLN AGVVDFEIFS
     ESRQGDIRAS VAEKIRELRR KQLVEETPKK NESNGNQDND EEELKSRSDL YKAKDVLGLY
     KVFPADINVK SEDAIDDSSA GGIICVGRGA YKEYLSGVHE GLLGPLEKPQ SVIDEETKLA
     EEKKKEKEEN PDKDDDNDEE QSNLKPVPLR YIKPEDYANA QLAPELDLST VVKDDKGVPV
     FFEQPVYTFP LPNLVGFTNI PRKIYRYFTK RFLVDDFGER TATIVNNKSR PFVYKDVLMA
     KEEEMDWPKK WVEKGKERNS EWVQELEHDE RVTSRMKVFE