ID   TIM54_CANGA             Reviewed;         452 AA.
AC   Q6FPC0;
DT   21-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 85.
DE   RecName: Full=Mitochondrial import inner membrane translocase subunit TIM54;
GN   Name=TIM54; OrderedLocusNames=CAGL0J05016g;
OS   Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL
OS   Y-65) (Yeast) (Torulopsis glabrata).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Nakaseomyces;
OC   Nakaseomyces/Candida clade.
OX   NCBI_TaxID=284593;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Essential component of the TIM22 complex, a complex that
CC       mediates the import and insertion of multi-pass transmembrane proteins
CC       into the mitochondrial inner membrane. The TIM22 complex forms a twin-
CC       pore translocase that uses the membrane potential as external driving
CC       force (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the TIM22 complex, whose core is composed of
CC       TIM22 and TIM54, associated with the 70 kDa heterohexamer composed of
CC       TIM9 and TIM10 (or TIM8 and TIM13). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000250};
CC       Single-pass membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the TIM54 family. {ECO:0000305}.
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DR   EMBL; CR380956; CAG60873.1; -; Genomic_DNA.
DR   RefSeq; XP_447924.1; XM_447924.1.
DR   AlphaFoldDB; Q6FPC0; -.
DR   SMR; Q6FPC0; -.
DR   STRING; 5478.XP_447924.1; -.
DR   EnsemblFungi; CAG60873; CAG60873; CAGL0J05016g.
DR   GeneID; 2889566; -.
DR   KEGG; cgr:CAGL0J05016g; -.
DR   CGD; CAL0133350; TIM54.
DR   VEuPathDB; FungiDB:CAGL0J05016g; -.
DR   eggNOG; ENOG502QPMQ; Eukaryota.
DR   HOGENOM; CLU_039097_0_0_1; -.
DR   InParanoid; Q6FPC0; -.
DR   OMA; EEKEWHK; -.
DR   Proteomes; UP000002428; Chromosome J.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0042721; C:TIM22 mitochondrial import inner membrane insertion complex; IEA:EnsemblFungi.
DR   GO; GO:0008320; F:protein transmembrane transporter activity; IEA:EnsemblFungi.
DR   GO; GO:0045039; P:protein insertion into mitochondrial inner membrane; IEA:EnsemblFungi.
DR   InterPro; IPR021056; Mt_import_IM_translocase_Tim54.
DR   Pfam; PF11711; Tim54; 1.
PE   3: Inferred from homology;
KW   Membrane; Mitochondrion; Mitochondrion inner membrane; Protein transport;
KW   Reference proteome; Translocation; Transmembrane; Transmembrane helix;
KW   Transport.
FT   CHAIN           1..452
FT                   /note="Mitochondrial import inner membrane translocase
FT                   subunit TIM54"
FT                   /id="PRO_0000228013"
FT   TOPO_DOM        1..19
FT                   /note="Mitochondrial matrix"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        20..36
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        37..452
FT                   /note="Mitochondrial intermembrane"
FT                   /evidence="ECO:0000255"
FT   REGION          271..299
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        272..299
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   452 AA;  51732 MW;  A3764A955BDE0C74 CRC64;
     MNPALKALGL GNFKLPSRNW TIFWTVLAGS LGGVGYDKYQ QRQIISRYCD EVKPLSLQHC
     DVNKSPRKIT VFIAPPPNDY LETSLKIWRR YIKPVLYYAG LDYEVIEEDR QGIIRSEVAS
     RIRQLRRELL EADNEQQGNS GDSLLSIFKK PHAKDPEEEQ KFDPEQARQF KADFDFRNVM
     GIYTKVPKLD TIVQTDSLVA DPVLAGGVVC VGRGAYKEYI TGLHEGLLGP LDPPEETTQE
     VEMGSKLKSM NDGDNVETTV ETAVETMVET TLESADKVEV EGKDTENEEG KDEPDEEKSR
     VLKPYLLRTA FHDTPIPPEV EPVLEKDALL KDPKTNVPSL LHQPVLVIPV PNLIGFLTIP
     ERIYRFYQRR FFVDEVCREA SNLVKQEHIV KYEPDKHINL ALEEESDWPK QWVKTGIEKN
     SEWTQELVQD QRVVSKMHVF ELPKHTTKDT KE