TIM54_CRYNB
ID TIM54_CRYNB Reviewed; 458 AA.
AC P0CR91; Q55LT0; Q5K8Z5;
DT 28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 25-MAY-2022, entry version 30.
DE RecName: Full=Mitochondrial import inner membrane translocase subunit TIM54;
GN Name=TIM54; OrderedLocusNames=CNBI2820;
OS Cryptococcus neoformans var. neoformans serotype D (strain B-3501A)
OS (Filobasidiella neoformans).
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes;
OC Tremellales; Cryptococcaceae; Cryptococcus;
OC Cryptococcus neoformans species complex.
OX NCBI_TaxID=283643;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=B-3501A;
RX PubMed=15653466; DOI=10.1126/science.1103773;
RA Loftus B.J., Fung E., Roncaglia P., Rowley D., Amedeo P., Bruno D.,
RA Vamathevan J., Miranda M., Anderson I.J., Fraser J.A., Allen J.E.,
RA Bosdet I.E., Brent M.R., Chiu R., Doering T.L., Donlin M.J., D'Souza C.A.,
RA Fox D.S., Grinberg V., Fu J., Fukushima M., Haas B.J., Huang J.C.,
RA Janbon G., Jones S.J.M., Koo H.L., Krzywinski M.I., Kwon-Chung K.J.,
RA Lengeler K.B., Maiti R., Marra M.A., Marra R.E., Mathewson C.A.,
RA Mitchell T.G., Pertea M., Riggs F.R., Salzberg S.L., Schein J.E.,
RA Shvartsbeyn A., Shin H., Shumway M., Specht C.A., Suh B.B., Tenney A.,
RA Utterback T.R., Wickes B.L., Wortman J.R., Wye N.H., Kronstad J.W.,
RA Lodge J.K., Heitman J., Davis R.W., Fraser C.M., Hyman R.W.;
RT "The genome of the basidiomycetous yeast and human pathogen Cryptococcus
RT neoformans.";
RL Science 307:1321-1324(2005).
CC -!- FUNCTION: Essential component of the TIM22 complex, a complex that
CC mediates the import and insertion of multi-pass transmembrane proteins
CC into the mitochondrial inner membrane. The TIM22 complex forms a twin-
CC pore translocase that uses the membrane potential as external driving
CC force (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of the TIM22 complex, whose core is composed of
CC TIM22 and TIM54, associated with the 70 kDa heterohexamer composed of
CC TIM9 and TIM10 (or TIM8 and TIM13). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000250};
CC Single-pass membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the TIM54 family. {ECO:0000305}.
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DR EMBL; AAEY01000046; EAL18694.1; -; Genomic_DNA.
DR RefSeq; XP_773341.1; XM_768248.1.
DR AlphaFoldDB; P0CR91; -.
DR EnsemblFungi; AAW46435; AAW46435; CNL03940.
DR EnsemblFungi; EAL18694; EAL18694; CNBI2820.
DR GeneID; 4938243; -.
DR KEGG; cnb:CNBI2820; -.
DR VEuPathDB; FungiDB:CNBI2820; -.
DR HOGENOM; CLU_033744_0_0_1; -.
DR Proteomes; UP000001435; Chromosome 9.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR InterPro; IPR021056; Mt_import_IM_translocase_Tim54.
DR Pfam; PF11711; Tim54; 1.
PE 3: Inferred from homology;
KW Membrane; Mitochondrion; Mitochondrion inner membrane; Protein transport;
KW Translocation; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..458
FT /note="Mitochondrial import inner membrane translocase
FT subunit TIM54"
FT /id="PRO_0000410307"
FT TOPO_DOM 1..43
FT /note="Mitochondrial matrix"
FT /evidence="ECO:0000255"
FT TRANSMEM 44..60
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 61..458
FT /note="Mitochondrial intermembrane"
FT /evidence="ECO:0000255"
SQ SEQUENCE 458 AA; 51693 MW; B6B62B3DAE75566B CRC64;
MADLTPGARK PAPAELTGFR SALAHTGIPH GVLLWKPRLP SRNWLVFWSV SLSLSYAYYY
DRAECKRIKQ EVVERVEKYG REPMPGGSLG EPRRVVVWAG RWGGDDDADR AGRYFRKYVK
PYLVAAGIDY TLPSVPLHGS ITRQLHAAIL LQRRQALGLA PTATPLSLPG VLDPAEAKRR
EVESGVVVVG RASLKEYLEG LRRGWECGVD EWAWETEVEK TLAGDGVFES VESPVEPAVE
TAETVVEPTA DAVPKSNFGF LARPAPVTPG APAIPAHLHT PPSPLPPTPP LLLLPFTNHL
GFLQLPYMIL DFFNERAKVR QGAQSALALI EGPTRDMHRE DAEHWEEKSE SWYNKTARQL
PERLQKSRTE YYEAIKSRID LARAYENGDR EMTEEEKKAN KVERIQDIQA ERLKKELRWK
GSEEGWEIVK PETPATWRDR WEGWLKVYQV PEDAQKGL