ID   TIM54_CRYNJ             Reviewed;         458 AA.
AC   P0CR90; Q55LT0; Q5K8Z5;
DT   28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT   28-JUN-2011, sequence version 1.
DT   25-MAY-2022, entry version 36.
DE   RecName: Full=Mitochondrial import inner membrane translocase subunit TIM54;
GN   Name=TIM54; OrderedLocusNames=CNL03940;
OS   Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC
OS   MYA-565) (Filobasidiella neoformans).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes;
OC   Tremellales; Cryptococcaceae; Cryptococcus;
OC   Cryptococcus neoformans species complex.
OX   NCBI_TaxID=214684;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JEC21 / ATCC MYA-565;
RX   PubMed=15653466; DOI=10.1126/science.1103773;
RA   Loftus B.J., Fung E., Roncaglia P., Rowley D., Amedeo P., Bruno D.,
RA   Vamathevan J., Miranda M., Anderson I.J., Fraser J.A., Allen J.E.,
RA   Bosdet I.E., Brent M.R., Chiu R., Doering T.L., Donlin M.J., D'Souza C.A.,
RA   Fox D.S., Grinberg V., Fu J., Fukushima M., Haas B.J., Huang J.C.,
RA   Janbon G., Jones S.J.M., Koo H.L., Krzywinski M.I., Kwon-Chung K.J.,
RA   Lengeler K.B., Maiti R., Marra M.A., Marra R.E., Mathewson C.A.,
RA   Mitchell T.G., Pertea M., Riggs F.R., Salzberg S.L., Schein J.E.,
RA   Shvartsbeyn A., Shin H., Shumway M., Specht C.A., Suh B.B., Tenney A.,
RA   Utterback T.R., Wickes B.L., Wortman J.R., Wye N.H., Kronstad J.W.,
RA   Lodge J.K., Heitman J., Davis R.W., Fraser C.M., Hyman R.W.;
RT   "The genome of the basidiomycetous yeast and human pathogen Cryptococcus
RT   neoformans.";
RL   Science 307:1321-1324(2005).
CC   -!- FUNCTION: Essential component of the TIM22 complex, a complex that
CC       mediates the import and insertion of multi-pass transmembrane proteins
CC       into the mitochondrial inner membrane. The TIM22 complex forms a twin-
CC       pore translocase that uses the membrane potential as external driving
CC       force (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the TIM22 complex, whose core is composed of
CC       TIM22 and TIM54, associated with the 70 kDa heterohexamer composed of
CC       TIM9 and TIM10 (or TIM8 and TIM13). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000250};
CC       Single-pass membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the TIM54 family. {ECO:0000305}.
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DR   EMBL; AE017352; AAW46435.1; -; Genomic_DNA.
DR   RefSeq; XP_567952.1; XM_567952.1.
DR   AlphaFoldDB; P0CR90; -.
DR   STRING; 5207.AAW46435; -.
DR   PaxDb; P0CR90; -.
DR   EnsemblFungi; AAW46435; AAW46435; CNL03940.
DR   GeneID; 3254900; -.
DR   KEGG; cne:CNL03940; -.
DR   VEuPathDB; FungiDB:CNL03940; -.
DR   eggNOG; ENOG502QPMQ; Eukaryota.
DR   HOGENOM; CLU_033744_0_0_1; -.
DR   InParanoid; P0CR90; -.
DR   OMA; EEKEWHK; -.
DR   OrthoDB; 960376at2759; -.
DR   Proteomes; UP000002149; Chromosome 12.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0042721; C:TIM22 mitochondrial import inner membrane insertion complex; IBA:GO_Central.
DR   GO; GO:0045039; P:protein insertion into mitochondrial inner membrane; IBA:GO_Central.
DR   InterPro; IPR021056; Mt_import_IM_translocase_Tim54.
DR   Pfam; PF11711; Tim54; 1.
PE   3: Inferred from homology;
KW   Membrane; Mitochondrion; Mitochondrion inner membrane; Protein transport;
KW   Reference proteome; Translocation; Transmembrane; Transmembrane helix;
KW   Transport.
FT   CHAIN           1..458
FT                   /note="Mitochondrial import inner membrane translocase
FT                   subunit TIM54"
FT                   /id="PRO_0000228014"
FT   TOPO_DOM        1..43
FT                   /note="Mitochondrial matrix"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        44..60
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        61..458
FT                   /note="Mitochondrial intermembrane"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   458 AA;  51693 MW;  B6B62B3DAE75566B CRC64;
     MADLTPGARK PAPAELTGFR SALAHTGIPH GVLLWKPRLP SRNWLVFWSV SLSLSYAYYY
     DRAECKRIKQ EVVERVEKYG REPMPGGSLG EPRRVVVWAG RWGGDDDADR AGRYFRKYVK
     PYLVAAGIDY TLPSVPLHGS ITRQLHAAIL LQRRQALGLA PTATPLSLPG VLDPAEAKRR
     EVESGVVVVG RASLKEYLEG LRRGWECGVD EWAWETEVEK TLAGDGVFES VESPVEPAVE
     TAETVVEPTA DAVPKSNFGF LARPAPVTPG APAIPAHLHT PPSPLPPTPP LLLLPFTNHL
     GFLQLPYMIL DFFNERAKVR QGAQSALALI EGPTRDMHRE DAEHWEEKSE SWYNKTARQL
     PERLQKSRTE YYEAIKSRID LARAYENGDR EMTEEEKKAN KVERIQDIQA ERLKKELRWK
     GSEEGWEIVK PETPATWRDR WEGWLKVYQV PEDAQKGL