TIM54_NEUCR
ID TIM54_NEUCR Reviewed; 468 AA.
AC Q9C0Q7;
DT 21-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT 15-JAN-2008, sequence version 2.
DT 25-MAY-2022, entry version 87.
DE RecName: Full=Mitochondrial import inner membrane translocase subunit tim54;
DE Flags: Precursor;
GN Name=tim54; ORFNames=NCU07295;
OS Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 /
OS FGSC 987).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Sordariomycetidae; Sordariales; Sordariaceae; Neurospora.
OX NCBI_TaxID=367110;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RX PubMed=14668492; DOI=10.1091/mbc.e03-05-0272;
RA Vasiljev A., Ahting U., Nargang F.E., Go N.E., Habib S.J., Kozany C.,
RA Panneels V., Sinning I., Prokisch H., Neupert W., Nussberger S.,
RA Rapaport D.;
RT "Reconstituted TOM core complex and Tim9/Tim10 complex of mitochondria are
RT sufficient for translocation of the ADP/ATP carrier across membranes.";
RL Mol. Biol. Cell 15:1445-1458(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX PubMed=12712197; DOI=10.1038/nature01554;
RA Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D.,
RA Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B.,
RA Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M.,
RA Qui D., Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M.,
RA Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U.,
RA Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D.,
RA Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S.,
RA Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D.,
RA Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S.,
RA Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A.,
RA DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R.,
RA Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R.,
RA Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I.,
RA Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.;
RT "The genome sequence of the filamentous fungus Neurospora crassa.";
RL Nature 422:859-868(2003).
CC -!- FUNCTION: Essential component of the TIM22 complex, a complex that
CC mediates the import and insertion of multi-pass transmembrane proteins
CC into the mitochondrial inner membrane. The TIM22 complex forms a twin-
CC pore translocase that uses the membrane potential as external driving
CC force (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of the TIM22 complex, whose core is composed of
CC tim22 and tim54, associated with the 70 kDa heterohexamer composed of
CC tim9 and tim10 (or tim8 and tim13). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000250};
CC Single-pass membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the TIM54 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAK26641.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=AAK26642.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AF343072; AAK26641.1; ALT_INIT; Genomic_DNA.
DR EMBL; AF343073; AAK26642.1; ALT_INIT; mRNA.
DR EMBL; CM002239; EAA32913.2; -; Genomic_DNA.
DR RefSeq; XP_962149.2; XM_957056.3.
DR AlphaFoldDB; Q9C0Q7; -.
DR SMR; Q9C0Q7; -.
DR STRING; 5141.EFNCRP00000007146; -.
DR EnsemblFungi; EAA32913; EAA32913; NCU07295.
DR GeneID; 3878298; -.
DR KEGG; ncr:NCU07295; -.
DR VEuPathDB; FungiDB:NCU07295; -.
DR HOGENOM; CLU_039097_1_0_1; -.
DR InParanoid; Q9C0Q7; -.
DR OMA; EEKEWHK; -.
DR Proteomes; UP000001805; Chromosome 4, Linkage Group IV.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0042721; C:TIM22 mitochondrial import inner membrane insertion complex; IBA:GO_Central.
DR GO; GO:0045039; P:protein insertion into mitochondrial inner membrane; IBA:GO_Central.
DR InterPro; IPR021056; Mt_import_IM_translocase_Tim54.
DR Pfam; PF11711; Tim54; 1.
PE 2: Evidence at transcript level;
KW Membrane; Mitochondrion; Mitochondrion inner membrane; Protein transport;
KW Reference proteome; Transit peptide; Translocation; Transmembrane;
KW Transmembrane helix; Transport.
FT TRANSIT 1..52
FT /note="Mitochondrion"
FT CHAIN 53..468
FT /note="Mitochondrial import inner membrane translocase
FT subunit tim54"
FT /id="PRO_0000228017"
FT TOPO_DOM 53..58
FT /note="Mitochondrial matrix"
FT /evidence="ECO:0000255"
FT TRANSMEM 59..75
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 76..468
FT /note="Mitochondrial intermembrane"
FT /evidence="ECO:0000255"
FT REGION 1..34
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 233..291
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 254..272
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 468 AA; 53075 MW; D21ADC2AAFDE3434 CRC64;
MADPVPPAST APPAAPAATT ATPPPPPPPP PPKALRPQNQ ALRMLGLPNL PNKLPSRNWM
IFWTVSASIT AAIIYDRREK RRNIAKWRHA VEHLAAEPIT DKLGLEQPRK LTIYLSAPPG
DGLRVAQDHY TEYVKPVLAA SGLDWEFVQG RREGDVRAVV AERLRKVRRG WENKEEQDPN
REPTKDELIE IYRQQRGIKD YEGVRGDVVI GRHTWKEYLR GLHEGWLGPL VAPAEPAPLP
PTPAPAAAEG STSTEDKPAE EKKEEEAPKP KRPPQPKPYN TTSDYSSETL HPLTPQELTP
AVPIREPHIL GFLNTPTRMV RFFNRRSLAD DIGREVAAVC LATHREFQQQ TNPDAPSTDS
VQYEQAKELE WEEQDWPKKV WKEDEADADK EVTEKIHIKP VVMDPRLAHR MRRFALTPED
EDRVSKIKVP EEEVEGWIKG SLRKACHWGY DKAFNKKKLV PLEDKDVE
