ID   TIM54_SCHPO             Reviewed;         347 AA.
AC   O94624;
DT   21-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   25-MAY-2022, entry version 103.
DE   RecName: Full=Mitochondrial import inner membrane translocase subunit tim54;
GN   Name=tim54; ORFNames=SPBC1347.04;
OS   Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC   Schizosaccharomyces.
OX   NCBI_TaxID=284812;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA   Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA   Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA   Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA   Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA   Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA   O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA   Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA   Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA   Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA   Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA   Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA   Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA   Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA   Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA   Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA   Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA   Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA   del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA   Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA   Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA   Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
CC   -!- FUNCTION: Essential component of the TIM22 complex, a complex that
CC       mediates the import and insertion of multi-pass transmembrane proteins
CC       into the mitochondrial inner membrane. The TIM22 complex forms a twin-
CC       pore translocase that uses the membrane potential as external driving
CC       force (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the TIM22 complex, whose core is composed of
CC       TIM22 and TIM54, associated with the 70 kDa heterohexamer composed of
CC       TIM9 and TIM10 (or TIM8 and TIM13). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000250};
CC       Single-pass membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the TIM54 family. {ECO:0000305}.
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DR   EMBL; CU329671; CAB37435.1; -; Genomic_DNA.
DR   PIR; T39392; T39392.
DR   RefSeq; NP_596696.1; NM_001022620.2.
DR   AlphaFoldDB; O94624; -.
DR   SMR; O94624; -.
DR   BioGRID; 276634; 1.
DR   STRING; 4896.SPBC1347.04.1; -.
DR   MaxQB; O94624; -.
DR   PaxDb; O94624; -.
DR   EnsemblFungi; SPBC1347.04.1; SPBC1347.04.1:pep; SPBC1347.04.
DR   GeneID; 2540096; -.
DR   KEGG; spo:SPBC1347.04; -.
DR   PomBase; SPBC1347.04; tim54.
DR   VEuPathDB; FungiDB:SPBC1347.04; -.
DR   eggNOG; ENOG502QPMQ; Eukaryota.
DR   HOGENOM; CLU_039097_0_0_1; -.
DR   InParanoid; O94624; -.
DR   OMA; EEKEWHK; -.
DR   PhylomeDB; O94624; -.
DR   Reactome; R-SPO-1483206; Glycerophospholipid biosynthesis.
DR   Reactome; R-SPO-1660661; Sphingolipid de novo biosynthesis.
DR   PRO; PR:O94624; -.
DR   Proteomes; UP000002485; Chromosome II.
DR   GO; GO:0016021; C:integral component of membrane; ISS:PomBase.
DR   GO; GO:0042721; C:TIM22 mitochondrial import inner membrane insertion complex; ISS:PomBase.
DR   GO; GO:0045039; P:protein insertion into mitochondrial inner membrane; ISS:PomBase.
DR   InterPro; IPR021056; Mt_import_IM_translocase_Tim54.
DR   Pfam; PF11711; Tim54; 1.
PE   3: Inferred from homology;
KW   Membrane; Mitochondrion; Mitochondrion inner membrane; Protein transport;
KW   Reference proteome; Translocation; Transmembrane; Transmembrane helix;
KW   Transport.
FT   CHAIN           1..347
FT                   /note="Mitochondrial import inner membrane translocase
FT                   subunit tim54"
FT                   /id="PRO_0000228018"
FT   TOPO_DOM        1..12
FT                   /note="Mitochondrial matrix"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        13..30
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        31..347
FT                   /note="Mitochondrial intermembrane"
FT                   /evidence="ECO:0000255"
FT   REGION          181..216
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   347 AA;  39803 MW;  EA3F0A27C2A76F02 CRC64;
     MLKTIKSYMP GRNMSIFLGF VAGISGAIYY DRRQKNLIIE KYCSQVRHLA DQPMAPLELP
     RKLKVYLHGP PGDGIYVARE EFEEYIRPIF NAAAIEFETV ESKGEGNLLE QVARTVYNKR
     HNISEVSEPE KNLLSVLKPS VDPPAIVLLG RHALKEFLYG VRYGFSDDIM KRKLETEKLE
     ANNKEEKEEK EGKDDKDDKE DSNDTKNDKK ISKNEVDSSL IEASPLTGQV PPKFLDTIAI
     FPLPNLLGFS NTPKRLSRFF KRRELADELG AIAVNVALSR DVTKFPKQDG TLLLAEEETD
     WPKQFFTRSD LENRIWTAPF LQDSDEIRFF ENIDIFDSTK AKQDKYE