ID   TIM54_YARLI             Reviewed;         572 AA.
AC   Q6C7Y5;
DT   21-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   25-MAY-2022, entry version 77.
DE   RecName: Full=Mitochondrial import inner membrane translocase subunit TIM54;
GN   Name=TIM54; OrderedLocusNames=YALI0D24343g;
OS   Yarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Dipodascaceae; Yarrowia.
OX   NCBI_TaxID=284591;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CLIB 122 / E 150;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Essential component of the TIM22 complex, a complex that
CC       mediates the import and insertion of multi-pass transmembrane proteins
CC       into the mitochondrial inner membrane. The TIM22 complex forms a twin-
CC       pore translocase that uses the membrane potential as external driving
CC       force (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the TIM22 complex, whose core is composed of
CC       TIM22 and TIM54, associated with the 70 kDa heterohexamer composed of
CC       TIM9 and TIM10 (or TIM8 and TIM13). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000250};
CC       Single-pass membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the TIM54 family. {ECO:0000305}.
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DR   EMBL; CR382130; CAG81428.1; -; Genomic_DNA.
DR   RefSeq; XP_503227.1; XM_503227.1.
DR   AlphaFoldDB; Q6C7Y5; -.
DR   STRING; 4952.CAG81428; -.
DR   EnsemblFungi; CAG81428; CAG81428; YALI0_D24343g.
DR   GeneID; 2910343; -.
DR   KEGG; yli:YALI0D24343g; -.
DR   VEuPathDB; FungiDB:YALI0_D24343g; -.
DR   HOGENOM; CLU_039097_0_0_1; -.
DR   InParanoid; Q6C7Y5; -.
DR   OMA; HEEREWH; -.
DR   Proteomes; UP000001300; Chromosome D.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0042721; C:TIM22 mitochondrial import inner membrane insertion complex; IBA:GO_Central.
DR   GO; GO:0045039; P:protein insertion into mitochondrial inner membrane; IBA:GO_Central.
DR   InterPro; IPR021056; Mt_import_IM_translocase_Tim54.
DR   Pfam; PF11711; Tim54; 1.
PE   3: Inferred from homology;
KW   Membrane; Mitochondrion; Mitochondrion inner membrane; Protein transport;
KW   Reference proteome; Translocation; Transmembrane; Transmembrane helix;
KW   Transport.
FT   CHAIN           1..572
FT                   /note="Mitochondrial import inner membrane translocase
FT                   subunit TIM54"
FT                   /id="PRO_0000228019"
FT   TOPO_DOM        1..42
FT                   /note="Mitochondrial matrix"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        43..59
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        60..572
FT                   /note="Mitochondrial intermembrane"
FT                   /evidence="ECO:0000255"
FT   REGION          1..21
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          185..221
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          363..404
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        191..221
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        363..383
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   572 AA;  63625 MW;  FD5F8EA83431189E CRC64;
     MADETAPKSS KPTAEAAPKK TGYTNPALRA MGIRQLRLPS RNWMIFWTVV GTISGGIYYD
     RHERKKVRQQ YKDAVAYLGE RPLGGLEIPR KITVFVAPPP GDYLDHVLTH FRAYIKPILT
     AAALDFEVKQ ESRQGEIRYV VAEGIRNYRR EQMGLPKVPS RLMVDEEEYN KAVAEQNAEV
     ERLKAGREKH TNPNPTFQAF NSINNRPGAP SSPDSQPEEQ MSISVAGTIA QEQLDKEITS
     KLEFNPESGV ICVGRGAYKE YLAGLHEGWL GPLEDPRPDE DNRKVYEEFP NREKDDDDAL
     GLVKTPVEEP TTAVQNVQSV SEHLNSHPEM VKDVSETISH SGDVTAVTDV EQTAAGPVDV
     VSETASTHDT PAASDNKTPK PLSEMSPSEW DPKAPLPEIK RKPVPKPYIR PEEYSEAELS
     EFYASGFENA STAHSTVSSN INQQPNDLNS PAGSGTAFVE QFFFSPIAVI PHRHIMGFMN
     TPLRIARYFN KRAVADEVGA ATVVAVTGDT RPFDVKTDPD LLVSEEYDWP SKWVKKGQDN
     GSEWVQPVVV DQRVMEKVKV YQPKGEGESE LK