TIM8A_XENLA
ID TIM8A_XENLA Reviewed; 94 AA.
AC Q66L32;
DT 21-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2004, sequence version 1.
DT 03-AUG-2022, entry version 77.
DE RecName: Full=Mitochondrial import inner membrane translocase subunit Tim8 A;
GN Name=timm8a; Synonyms=tim8a;
OS Xenopus laevis (African clawed frog).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX NCBI_TaxID=8355;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RG NIH - Xenopus Gene Collection (XGC) project;
RL Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Mitochondrial intermembrane chaperone that participates in
CC the import and insertion of some multi-pass transmembrane proteins into
CC the mitochondrial inner membrane. Also required for the transfer of
CC beta-barrel precursors from the TOM complex to the sorting and assembly
CC machinery (SAM complex) of the outer membrane. Acts as a chaperone-like
CC protein that protects the hydrophobic precursors from aggregation and
CC guide them through the mitochondrial intermembrane space. The TIMM8-
CC TIMM13 complex mediates the import of some proteins while the
CC predominant TIMM9-TIMM10 70 kDa complex mediates the import of much
CC more proteins (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Heterohexamer; composed of 3 copies of TIMM8A and 3 copies of
CC TIMM13, named soluble 70 kDa complex. Associates with the TIM22
CC complex, whose core is composed of TIMM22 (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000250};
CC Peripheral membrane protein {ECO:0000250}; Intermembrane side
CC {ECO:0000250}.
CC -!- DOMAIN: The twin CX3C motif contains 4 conserved Cys residues that form
CC 2 disulfide bonds in the mitochondrial intermembrane space. However,
CC during the transit of TIMM8A from cytoplasm into mitochondrion, the Cys
CC residues probably coordinate zinc, thereby preventing folding and
CC allowing its transfer across mitochondrial outer membrane (By
CC similarity). {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the small Tim family. {ECO:0000305}.
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DR EMBL; BC078465; AAH78465.1; -; mRNA.
DR RefSeq; NP_001087186.1; NM_001093717.1.
DR AlphaFoldDB; Q66L32; -.
DR SMR; Q66L32; -.
DR DNASU; 447075; -.
DR GeneID; 447075; -.
DR KEGG; xla:447075; -.
DR CTD; 447075; -.
DR Xenbase; XB-GENE-6254561; timm8b.L.
DR OMA; SKQKVQM; -.
DR OrthoDB; 1593287at2759; -.
DR Proteomes; UP000186698; Chromosome 7L.
DR Bgee; 447075; Expressed in muscle tissue and 19 other tissues.
DR GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005758; C:mitochondrial intermembrane space; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR Gene3D; 1.10.287.810; -; 1.
DR InterPro; IPR004217; Tim10-like.
DR InterPro; IPR035427; Tim10-like_dom_sf.
DR InterPro; IPR039238; Tim8/13.
DR PANTHER; PTHR19338; PTHR19338; 1.
DR Pfam; PF02953; zf-Tim10_DDP; 1.
DR SUPFAM; SSF144122; SSF144122; 1.
PE 3: Inferred from homology;
KW Chaperone; Disulfide bond; Membrane; Metal-binding; Mitochondrion;
KW Mitochondrion inner membrane; Protein transport; Reference proteome;
KW Translocation; Transport; Zinc.
FT CHAIN 1..94
FT /note="Mitochondrial import inner membrane translocase
FT subunit Tim8 A"
FT /id="PRO_0000228024"
FT MOTIF 47..70
FT /note="Twin CX3C motif"
FT DISULFID 47..70
FT /evidence="ECO:0000250"
FT DISULFID 51..66
FT /evidence="ECO:0000250"
SQ SEQUENCE 94 AA; 10409 MW; 404CA99E9B0E72F6 CRC64;
MSDFDSNLDL AGTGASPAEA AELQRMLAVE QQKAQFTAQV HNFMDVCWDK CIDRPGNKLD
SRTESCLVSC VDRFIDTTLS ITNRFAQIVQ KGAH
