TIM8_CAEBR
ID TIM8_CAEBR Reviewed; 83 AA.
AC Q616Q2; A8XLR0;
DT 21-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT 23-NOV-2004, sequence version 1.
DT 03-AUG-2022, entry version 96.
DE RecName: Full=Mitochondrial import inner membrane translocase subunit Tim8 {ECO:0000250|UniProtKB:Q9Y1A3};
GN Name=ddp-1 {ECO:0000312|WormBase:CBG15185};
GN Synonyms=tim-3 {ECO:0000250|UniProtKB:Q9N408};
GN ORFNames=CBG15185 {ECO:0000312|WormBase:CBG15185};
OS Caenorhabditis briggsae.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6238;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AF16;
RX PubMed=14624247; DOI=10.1371/journal.pbio.0000045;
RA Stein L.D., Bao Z., Blasiar D., Blumenthal T., Brent M.R., Chen N.,
RA Chinwalla A., Clarke L., Clee C., Coghlan A., Coulson A., D'Eustachio P.,
RA Fitch D.H.A., Fulton L.A., Fulton R.E., Griffiths-Jones S., Harris T.W.,
RA Hillier L.W., Kamath R., Kuwabara P.E., Mardis E.R., Marra M.A.,
RA Miner T.L., Minx P., Mullikin J.C., Plumb R.W., Rogers J., Schein J.E.,
RA Sohrmann M., Spieth J., Stajich J.E., Wei C., Willey D., Wilson R.K.,
RA Durbin R.M., Waterston R.H.;
RT "The genome sequence of Caenorhabditis briggsae: a platform for comparative
RT genomics.";
RL PLoS Biol. 1:166-192(2003).
CC -!- FUNCTION: Mitochondrial intermembrane chaperone that participates in
CC the import and insertion of some multi-pass transmembrane proteins into
CC the mitochondrial inner membrane. Also required for the transfer of
CC beta-barrel precursors from the TOM complex to the sorting and assembly
CC machinery (SAM complex) of the outer membrane. Acts as a chaperone-like
CC protein that protects the hydrophobic precursors from aggregation and
CC guide them through the mitochondrial intermembrane space. The ddp-
CC 1/tim-8-tim-13 complex mediates the import of some proteins while the
CC predominant tim-9/tin-9.1-tim-10/tin-10 70 kDa complex mediates the
CC import of much more proteins. {ECO:0000250|UniProtKB:Q17754,
CC ECO:0000250|UniProtKB:Q9N408}.
CC -!- SUBUNIT: Heterohexamer; composed of 3 copies of ddp-1/tim-8 and 3
CC copies of tin-13/tim-13, named soluble 70 kDa complex. Associates with
CC the TIM22 complex, whose core is composed of tim-22 (By similarity).
CC {ECO:0000250|UniProtKB:O74700}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000250|UniProtKB:O74700}; Peripheral membrane protein
CC {ECO:0000250|UniProtKB:O74700}; Intermembrane side
CC {ECO:0000250|UniProtKB:O74700}.
CC -!- DOMAIN: The twin CX3C motif contains 4 conserved Cys residues that form
CC 2 disulfide bonds in the mitochondrial intermembrane space. However,
CC during the transit of ddp-1/tim-8 from cytoplasm into mitochondrion,
CC the Cys residues probably coordinate zinc, thereby preventing folding
CC and allowing its transfer across mitochondrial outer membrane.
CC {ECO:0000250|UniProtKB:O74700}.
CC -!- SIMILARITY: Belongs to the small Tim family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; HE601055; CAP33564.1; -; Genomic_DNA.
DR RefSeq; XP_002642910.1; XM_002642864.1.
DR AlphaFoldDB; Q616Q2; -.
DR SMR; Q616Q2; -.
DR STRING; 6238.CBG15185; -.
DR EnsemblMetazoa; CBG15185.1; CBG15185.1; WBGene00035508.
DR GeneID; 8584903; -.
DR KEGG; cbr:CBG_15185; -.
DR CTD; 8584903; -.
DR WormBase; CBG15185; CBP23176; WBGene00035508; Cbr-ddp-1.
DR eggNOG; KOG3489; Eukaryota.
DR HOGENOM; CLU_141397_1_2_1; -.
DR InParanoid; Q616Q2; -.
DR OMA; MADATEY; -.
DR OrthoDB; 1593287at2759; -.
DR Proteomes; UP000008549; Chromosome III.
DR GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005758; C:mitochondrial intermembrane space; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0007005; P:mitochondrion organization; IEA:EnsemblMetazoa.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR Gene3D; 1.10.287.810; -; 1.
DR InterPro; IPR004217; Tim10-like.
DR InterPro; IPR035427; Tim10-like_dom_sf.
DR InterPro; IPR039238; Tim8/13.
DR PANTHER; PTHR19338; PTHR19338; 1.
DR Pfam; PF02953; zf-Tim10_DDP; 1.
DR SUPFAM; SSF144122; SSF144122; 1.
PE 3: Inferred from homology;
KW Chaperone; Disulfide bond; Membrane; Metal-binding; Mitochondrion;
KW Mitochondrion inner membrane; Protein transport; Reference proteome;
KW Translocation; Transport; Zinc.
FT CHAIN 1..83
FT /note="Mitochondrial import inner membrane translocase
FT subunit Tim8"
FT /id="PRO_0000228026"
FT MOTIF 35..60
FT /note="Twin CX3C motif"
FT /evidence="ECO:0000250|UniProtKB:O74700"
FT DISULFID 35..60
FT /evidence="ECO:0000250|UniProtKB:O74700"
FT DISULFID 39..56
FT /evidence="ECO:0000250|UniProtKB:O74700"
SQ SEQUENCE 83 AA; 9482 MW; ACC513956CD10463 CRC64;
MDSADPQLAR FLHQLQAETQ RQKFTEQVHT LTGRCWDVCF SDYRPPSKMD GKTQTCIQNC
VNRMIDASNF MVEHLSKMNG GSA
