ID   TIM8_NEUCR              Reviewed;          92 AA.
AC   Q9Y8C0; A7UWR2; Q7SBT0;
DT   11-JAN-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1999, sequence version 1.
DT   25-MAY-2022, entry version 125.
DE   RecName: Full=Mitochondrial import inner membrane translocase subunit tim8;
GN   Name=tim8; ORFNames=NCU06225, NCU11311;
OS   Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 /
OS   FGSC 987).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Sordariomycetidae; Sordariales; Sordariaceae; Neurospora.
OX   NCBI_TaxID=367110;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=10611480; DOI=10.1016/s0014-5793(99)01665-8;
RA   Bauer M.F., Rothbauer U., Muehlenbein N., Smith R.J.H., Gerbitz K.-D.,
RA   Neupert W., Brunner M., Hofmann S.;
RT   "The mitochondrial TIM22 preprotein translocase is highly conserved
RT   throughout the eukaryotic kingdom.";
RL   FEBS Lett. 464:41-47(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX   PubMed=12712197; DOI=10.1038/nature01554;
RA   Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D.,
RA   Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B.,
RA   Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M.,
RA   Qui D., Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M.,
RA   Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U.,
RA   Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D.,
RA   Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S.,
RA   Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D.,
RA   Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S.,
RA   Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A.,
RA   DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R.,
RA   Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R.,
RA   Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I.,
RA   Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.;
RT   "The genome sequence of the filamentous fungus Neurospora crassa.";
RL   Nature 422:859-868(2003).
RN   [3]
RP   FUNCTION IN TRANSFER OF BETA-BARREL PROTEINS, SUBCELLULAR LOCATION, AND
RP   SUBUNIT.
RX   PubMed=14722057; DOI=10.1074/jbc.m313037200;
RA   Hoppins S.C., Nargang F.E.;
RT   "The Tim8-Tim13 complex of Neurospora crassa functions in the assembly of
RT   proteins into both mitochondrial membranes.";
RL   J. Biol. Chem. 279:12396-12405(2004).
CC   -!- FUNCTION: Mitochondrial intermembrane chaperone that participates in
CC       the import and insertion of some multi-pass transmembrane proteins into
CC       the mitochondrial inner membrane. Also required for the transfer of
CC       beta-barrel precursors from the TOM complex to the sorting and assembly
CC       machinery (SAM complex) of the outer membrane. Acts as a chaperone-like
CC       protein that protects the hydrophobic precursors from aggregation and
CC       guide them through the mitochondrial intermembrane space. The tim8-
CC       tim13 complex is non essential and only mediates the import of few
CC       proteins, while the predominant tim9-tim10 70 kDa complex is crucial
CC       and mediates the import of much more proteins.
CC       {ECO:0000269|PubMed:14722057}.
CC   -!- SUBUNIT: Heterohexamer; composed of 3 copies of tim8 and 3 copies of
CC       tim13, named soluble 70 kDa complex. Associates with the TIM22 complex,
CC       whose core is composed of tim22 and tim54. Interacts with the
CC       transmembrane regions of multi-pass transmembrane proteins in transit.
CC       {ECO:0000269|PubMed:14722057}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC       {ECO:0000269|PubMed:14722057}; Peripheral membrane protein
CC       {ECO:0000269|PubMed:14722057}; Intermembrane side
CC       {ECO:0000269|PubMed:14722057}.
CC   -!- DOMAIN: The twin CX3C motif contains 4 conserved Cys residues that form
CC       2 disulfide bonds in the mitochondrial intermembrane space. However,
CC       during the transit of tim8 from cytoplasm into mitochondrion, the Cys
CC       residues probably coordinate zinc, thereby preventing folding and
CC       allowing its transfer across mitochondrial outer membrane (By
CC       similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the small Tim family. {ECO:0000305}.
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DR   EMBL; AF142423; AAD39161.1; -; mRNA.
DR   EMBL; CM002238; EDO65121.1; -; Genomic_DNA.
DR   RefSeq; XP_001728212.1; XM_001728160.2.
DR   AlphaFoldDB; Q9Y8C0; -.
DR   SMR; Q9Y8C0; -.
DR   STRING; 5141.EFNCRP00000005941; -.
DR   EnsemblFungi; EDO65121; EDO65121; NCU11311.
DR   GeneID; 5847395; -.
DR   KEGG; ncr:NCU11311; -.
DR   VEuPathDB; FungiDB:NCU11311; -.
DR   HOGENOM; CLU_141397_1_0_1; -.
DR   InParanoid; Q9Y8C0; -.
DR   OMA; SKQKVQM; -.
DR   Proteomes; UP000001805; Chromosome 3, Linkage Group III.
DR   GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005758; C:mitochondrial intermembrane space; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.287.810; -; 1.
DR   InterPro; IPR004217; Tim10-like.
DR   InterPro; IPR035427; Tim10-like_dom_sf.
DR   InterPro; IPR039238; Tim8/13.
DR   PANTHER; PTHR19338; PTHR19338; 1.
DR   Pfam; PF02953; zf-Tim10_DDP; 1.
DR   SUPFAM; SSF144122; SSF144122; 1.
PE   1: Evidence at protein level;
KW   Chaperone; Disulfide bond; Membrane; Metal-binding; Mitochondrion;
KW   Mitochondrion inner membrane; Protein transport; Reference proteome;
KW   Translocation; Transport; Zinc.
FT   CHAIN           1..92
FT                   /note="Mitochondrial import inner membrane translocase
FT                   subunit tim8"
FT                   /id="PRO_0000193592"
FT   MOTIF           43..68
FT                   /note="Twin CX3C motif"
FT   DISULFID        43..68
FT                   /evidence="ECO:0000250"
FT   DISULFID        47..64
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   92 AA;  10420 MW;  B5101B8EF0FF1DCD CRC64;
     MDIPQADLDL LNEKDKNELR GFISNETQRQ RVQGQTHALT DSCWKKCVTS PIKTNQLDKT
     EAVCMADCVE RFLDVNLTIM AHVQKITRGG SK