ID   TIM9_CAEBR              Reviewed;          78 AA.
AC   Q61TH2; A8X1Q4;
DT   21-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT   23-NOV-2004, sequence version 1.
DT   03-AUG-2022, entry version 100.
DE   RecName: Full=Mitochondrial import inner membrane translocase subunit Tim9;
GN   Name=tin-9.1; Synonyms=tin-9; ORFNames=CBG05752;
OS   Caenorhabditis briggsae.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6238;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AF16;
RX   PubMed=14624247; DOI=10.1371/journal.pbio.0000045;
RA   Stein L.D., Bao Z., Blasiar D., Blumenthal T., Brent M.R., Chen N.,
RA   Chinwalla A., Clarke L., Clee C., Coghlan A., Coulson A., D'Eustachio P.,
RA   Fitch D.H.A., Fulton L.A., Fulton R.E., Griffiths-Jones S., Harris T.W.,
RA   Hillier L.W., Kamath R., Kuwabara P.E., Mardis E.R., Marra M.A.,
RA   Miner T.L., Minx P., Mullikin J.C., Plumb R.W., Rogers J., Schein J.E.,
RA   Sohrmann M., Spieth J., Stajich J.E., Wei C., Willey D., Wilson R.K.,
RA   Durbin R.M., Waterston R.H.;
RT   "The genome sequence of Caenorhabditis briggsae: a platform for comparative
RT   genomics.";
RL   PLoS Biol. 1:166-192(2003).
CC   -!- FUNCTION: Mitochondrial intermembrane chaperone that participates in
CC       the import and insertion of multi-pass transmembrane proteins into the
CC       mitochondrial inner membrane. May also be required for the transfer of
CC       beta-barrel precursors from the TOM complex to the sorting and assembly
CC       machinery (SAM complex) of the outer membrane. Acts as a chaperone-like
CC       protein that protects the hydrophobic precursors from aggregation and
CC       guide them through the mitochondrial intermembrane space (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Heterohexamer; composed of 3 copies of tim-9/tin-9.1 and 3
CC       copies of tim-10/tin-10, named soluble 70 kDa complex. The complex
CC       associates with the tim-22 component of the TIM22 complex. Interacts
CC       with multi-pass transmembrane proteins in transit (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000250};
CC       Peripheral membrane protein {ECO:0000250}; Intermembrane side
CC       {ECO:0000250}.
CC   -!- DOMAIN: The twin CX3C motif contains 4 conserved Cys residues that form
CC       2 disulfide bonds in the mitochondrial intermembrane space. However,
CC       during the transit of tim-9/tin-9.1 from cytoplasm into mitochondrion,
CC       the Cys residues probably coordinate zinc, thereby preventing folding
CC       and allowing its transfer across mitochondrial outer membrane (By
CC       similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the small Tim family. {ECO:0000305}.
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DR   EMBL; HE600909; CAP26564.1; -; Genomic_DNA.
DR   RefSeq; XP_002633069.1; XM_002633023.1.
DR   AlphaFoldDB; Q61TH2; -.
DR   SMR; Q61TH2; -.
DR   STRING; 6238.CBG05752; -.
DR   EnsemblMetazoa; CBG05752.1; CBG05752.1; WBGene00028143.
DR   GeneID; 8575066; -.
DR   KEGG; cbr:CBG_05752; -.
DR   CTD; 8575066; -.
DR   WormBase; CBG05752; CBP01491; WBGene00028143; Cbr-tin-9.1.
DR   eggNOG; KOG3479; Eukaryota.
DR   HOGENOM; CLU_141397_3_3_1; -.
DR   InParanoid; Q61TH2; -.
DR   OMA; QDFLRMY; -.
DR   OrthoDB; 1627953at2759; -.
DR   Proteomes; UP000008549; Chromosome IV.
DR   GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0045039; P:protein insertion into mitochondrial inner membrane; IEA:EnsemblMetazoa.
DR   GO; GO:0040014; P:regulation of multicellular organism growth; IEA:EnsemblMetazoa.
DR   GO; GO:0000003; P:reproduction; IEA:EnsemblMetazoa.
DR   Gene3D; 1.10.287.810; -; 1.
DR   InterPro; IPR004217; Tim10-like.
DR   InterPro; IPR035427; Tim10-like_dom_sf.
DR   Pfam; PF02953; zf-Tim10_DDP; 1.
DR   SUPFAM; SSF144122; SSF144122; 1.
PE   3: Inferred from homology;
KW   Chaperone; Disulfide bond; Membrane; Metal-binding; Mitochondrion;
KW   Mitochondrion inner membrane; Protein transport; Reference proteome;
KW   Translocation; Transport; Zinc.
FT   CHAIN           1..78
FT                   /note="Mitochondrial import inner membrane translocase
FT                   subunit Tim9"
FT                   /id="PRO_0000228038"
FT   MOTIF           24..48
FT                   /note="Twin CX3C motif"
FT   DISULFID        24..48
FT                   /evidence="ECO:0000250"
FT   DISULFID        28..44
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   78 AA;  8900 MW;  920795028F1B4681 CRC64;
     MASEQNIQTF RDFLTQYNLV AEQCFTSCVN EFGSRTVNAK EESCANNCLD KFLKMTQRVS
     QRFQEHQILN AQANGAAM