ID   TIMP_ECOLI              Reviewed;          41 AA.
AC   P0DUM3;
DT   02-JUN-2021, integrated into UniProtKB/Swiss-Prot.
DT   02-JUN-2021, sequence version 1.
DT   25-MAY-2022, entry version 3.
DE   RecName: Full=Putative toxic protein TimP {ECO:0000250|UniProtKB:P0DUM2};
GN   Name=timP; OrderedLocusNames=b4812;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
CC   -!- FUNCTION: Toxic component of a probable type I toxin-antitoxin (TA)
CC       system. Neutralized by sRNA antitoxin TimR which binds to the 5' UTR of
CC       timP mRNA and inhibits translation. {ECO:0000250|UniProtKB:P0DUM2}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane
CC       {ECO:0000250|UniProtKB:P0DUM2}; Single-pass membrane protein
CC       {ECO:0000250|UniProtKB:P0DUM2}. Note=The signal sequence is not cleaved
CC       and anchors the protein in the cell inner membrane.
CC       {ECO:0000250|UniProtKB:P0DUM2}.
CC   -!- SIMILARITY: Belongs to the TimP toxin family. {ECO:0000305}.
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DR   EMBL; U00096; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   AlphaFoldDB; P0DUM3; -.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW   Toxin-antitoxin system; Transmembrane.
FT   CHAIN           1..41
FT                   /note="Putative toxic protein TimP"
FT                   /id="PRO_0000453138"
FT   TRANSMEM        1..17
FT                   /evidence="ECO:0000250|UniProtKB:P0DUM2"
SQ   SEQUENCE   41 AA;  4477 MW;  301B1523E10ED6AD CRC64;
     MKIRCFCIVL IVSGALLTEV NNNRSLSGDN LLVVNNLQSS K