ID   TIMP_SALTS              Reviewed;          38 AA.
AC   P0DUM2;
DT   02-JUN-2021, integrated into UniProtKB/Swiss-Prot.
DT   02-JUN-2021, sequence version 1.
DT   25-MAY-2022, entry version 3.
DE   RecName: Full=Toxic protein TimP {ECO:0000303|PubMed:33172998};
GN   Name=timP {ECO:0000303|PubMed:33172998};
GN   Synonyms=ryfA {ECO:0000303|PubMed:24331466};
GN   OrderedLocusNames=SL1344_2496.5 {ECO:0000305};
OS   Salmonella typhimurium (strain SL1344).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Salmonella.
OX   NCBI_TaxID=216597;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SL1344;
RX   PubMed=22538806; DOI=10.1073/pnas.1201061109;
RA   Kroger C., Dillon S.C., Cameron A.D., Papenfort K., Sivasankaran S.K.,
RA   Hokamp K., Chao Y., Sittka A., Hebrard M., Handler K., Colgan A.,
RA   Leekitcharoenphon P., Langridge G.C., Lohan A.J., Loftus B., Lucchini S.,
RA   Ussery D.W., Dorman C.J., Thomson N.R., Vogel J., Hinton J.C.;
RT   "The transcriptional landscape and small RNAs of Salmonella enterica
RT   serovar Typhimurium.";
RL   Proc. Natl. Acad. Sci. U.S.A. 109:E1277-E1286(2012).
RN   [2]
RP   INDUCTION.
RC   STRAIN=4/74;
RX   PubMed=24331466; DOI=10.1016/j.chom.2013.11.010;
RA   Kroeger C., Colgan A., Srikumar S., Haendler K., Sivasankaran S.K.,
RA   Hammarloef D.L., Canals R., Grissom J.E., Conway T., Hokamp K.,
RA   Hinton J.C.;
RT   "An infection-relevant transcriptomic compendium for Salmonella enterica
RT   Serovar Typhimurium.";
RL   Cell Host Microbe 14:683-695(2013).
RN   [3]
RP   IDENTIFICATION, FUNCTION, SUBCELLULAR LOCATION, INDUCTION, AND DISRUPTION
RP   PHENOTYPE.
RC   STRAIN=SL1344;
RX   PubMed=33172998; DOI=10.1128/mbio.01659-20;
RA   Andresen L., Martinez-Burgo Y., Nilsson Zangelin J., Rizvanovic A.,
RA   Holmqvist E.;
RT   "The Small Toxic Salmonella Protein TimP Targets the Cytoplasmic Membrane
RT   and Is Repressed by the Small RNA TimR.";
RL   MBio 11:0-0(2020).
CC   -!- FUNCTION: Toxic component of a probable type I toxin-antitoxin (TA)
CC       system. Neutralized by sRNA antitoxin TimR which binds to the 5' UTR of
CC       timP mRNA and inhibits translation. When TimP is expressed from its
CC       promoter in the absence of antitoxin leads to mild cell stress;
CC       overexpression in situ is toxic to the cell and causes membrane
CC       leakage. The antitoxin gene is encoded immediately upstream and
CC       transcribed divergently from the toxin gene; antitoxin RNA is less
CC       stable than timP mRNA. {ECO:0000269|PubMed:33172998}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane
CC       {ECO:0000269|PubMed:33172998}; Single-pass membrane protein
CC       {ECO:0000305|PubMed:33172998}. Note=The signal sequence is not cleaved
CC       and anchors the protein in the cell inner membrane.
CC       {ECO:0000305|PubMed:33172998}.
CC   -!- INDUCTION: Expressed under many growth conditions, up-regulated in
CC       macrophages. {ECO:0000269|PubMed:24331466}.
CC   -!- DISRUPTION PHENOTYPE: No changes in biofilm formation, persister cell
CC       formation or bacteriophage resistance; no visible phenotype for a
CC       double timR-timP deletion. A timR deletion leads to mild envelope
CC       stress. {ECO:0000269|PubMed:33172998}.
CC   -!- SIMILARITY: Belongs to the TimP toxin family. {ECO:0000305}.
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DR   EMBL; FQ312003; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   AlphaFoldDB; P0DUM2; -.
DR   Proteomes; UP000008962; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
PE   2: Evidence at transcript level;
KW   Cell inner membrane; Cell membrane; Membrane; Toxin-antitoxin system;
KW   Transmembrane.
FT   CHAIN           1..37
FT                   /note="Toxic protein TimP"
FT                   /id="PRO_0000453021"
FT   TRANSMEM        1..20
FT                   /evidence="ECO:0000305|PubMed:33172998"
SQ   SEQUENCE   38 AA;  4164 MW;  CE3A72C7DD273452 CRC64;
     MKVRCFCVVL LVSGTLCLHA DRSYPGNSVP VTLNVQSR