ID   TIN2_HOPTI              Reviewed;          12 AA.
AC   P82652;
DT   27-APR-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   25-MAY-2022, entry version 47.
DE   RecName: Full=Tigerinin-2;
OS   Hoplobatrachus tigerinus (Indian bull frog) (Rana tigerina).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Ranoidea; Dicroglossidae; Dicroglossinae;
OC   Hoplobatrachus.
OX   NCBI_TaxID=103373;
RN   [1]
RP   PROTEIN SEQUENCE, FUNCTION, AMIDATION AT HIS-12, MASS SPECTROMETRY, AND
RP   DISULFIDE BONDS.
RC   TISSUE=Skin secretion;
RX   PubMed=11031261; DOI=10.1074/jbc.m006615200;
RA   Purna Sai K., Jaganadham M.V., Vairamani M., Raju N.P., Devi A.S.,
RA   Nagaraj R., Sitaram N.;
RT   "Tigerinins: novel antimicrobial peptides from the Indian frog Rana
RT   tigerina.";
RL   J. Biol. Chem. 276:2701-2707(2001).
CC   -!- FUNCTION: Antibacterial activity against B.subtilis, E.coli, S.aureus,
CC       M.luteus and P.putida. Antifungal activity against S.cerevisiae.
CC       {ECO:0000269|PubMed:11031261}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed by the skin glands.
CC   -!- MASS SPECTROMETRY: Mass=1368; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:11031261};
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DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR   GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Amidation; Amphibian defense peptide; Antibiotic; Antimicrobial;
KW   Direct protein sequencing; Disulfide bond; Fungicide; Secreted.
FT   PEPTIDE         1..12
FT                   /note="Tigerinin-2"
FT                   /id="PRO_0000043839"
FT   MOD_RES         12
FT                   /note="Histidine amide"
FT                   /evidence="ECO:0000269|PubMed:11031261"
FT   DISULFID        3..11
FT                   /evidence="ECO:0000269|PubMed:11031261"
SQ   SEQUENCE   12 AA;  1369 MW;  C2627D8EFD37605D CRC64;
     RVCFAIPLPI CH