ID   TIN4O_HOPOC             Reviewed;          11 AA.
AC   C0HL44;
DT   25-OCT-2017, integrated into UniProtKB/Swiss-Prot.
DT   25-OCT-2017, sequence version 1.
DT   11-DEC-2019, entry version 3.
DE   RecName: Full=Tigerinin-4O {ECO:0000303|PubMed:27560386};
OS   Hoplobatrachus occipitalis (African groove-crowned bullfrog) (Rana
OS   occipitalis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Ranoidea; Dicroglossidae; Dicroglossinae;
OC   Hoplobatrachus.
OX   NCBI_TaxID=127645 {ECO:0000303|PubMed:27560386};
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, MASS
RP   SPECTROMETRY, AND DISULFIDE BOND.
RC   TISSUE=Skin secretion {ECO:0000303|PubMed:27560386};
RX   PubMed=27560386; DOI=10.1021/acs.jnatprod.6b00494;
RA   McLaughlin C.M., Lampis S., Mechkarska M., Coquet L., Jouenne T.,
RA   King J.D., Mangoni M.L., Lukic M.L., Scorciapino M.A., Conlon J.M.;
RT   "Purification, Conformational Analysis, and Properties of a Family of
RT   Tigerinin Peptides from Skin Secretions of the Crowned Bullfrog
RT   Hoplobatrachus occipitalis.";
RL   J. Nat. Prod. 79:2350-2356(2016).
CC   -!- FUNCTION: Stimulates insulin release from beta cells at a concentration
CC       of 1 uM and release of glucagon-like peptide 1 (GLP-1) from
CC       enteroendocrine cells at a concentration of 10 nM in vitro. Reduces
CC       secretion of interferon gamma from peritoneal cells in a mouse model.
CC       Has no inhibitory activity against Gram-positive bacterium B.megaterium
CC       Bm11 or Gram-negative bacterium E.coli ATCC 25922 at concentrations of
CC       up to 100 uM. Has no hemolytic activity against mouse erythrocytes.
CC       {ECO:0000269|PubMed:27560386}.
CC   -!- SUBUNIT: Homodimer; the relevance of this is unclear as other
CC       tigerinins do not form dimers and a synthetic peptide remained a
CC       monomer. {ECO:0000269|PubMed:27560386}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:27560386}.
CC   -!- TISSUE SPECIFICITY: Expressed by the skin glands.
CC       {ECO:0000305|PubMed:27560386}.
CC   -!- MASS SPECTROMETRY: Mass=2486.4; Method=MALDI; Note=Homodimer.;
CC       Evidence={ECO:0000269|PubMed:27560386};
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DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Amphibian defense peptide; Direct protein sequencing; Disulfide bond;
KW   Secreted.
FT   PEPTIDE         1..11
FT                   /note="Tigerinin-4O"
FT                   /evidence="ECO:0000269|PubMed:27560386"
FT                   /id="PRO_0000442178"
FT   DISULFID        3..10
FT                   /evidence="ECO:0000269|PubMed:27560386"
SQ   SEQUENCE   11 AA;  1246 MW;  1601E14D77604760 CRC64;
     RTCIPIPPVC F