BSH_MOUSE
ID BSH_MOUSE Reviewed; 232 AA.
AC Q810B3; Q3SXB1;
DT 31-AUG-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=Brain-specific homeobox protein homolog;
GN Name=Bsx {ECO:0000312|EMBL:AAO84023.1};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1] {ECO:0000312|EMBL:AAO84023.1}
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=CD-1 {ECO:0000312|EMBL:AAO84023.1};
RC TISSUE=Hypothalamus {ECO:0000269|Ref.1}, and
RC Pineal gland {ECO:0000269|Ref.1};
RA Assimacopoulos S., Ragsdale C.W.;
RT "Identification of vertebrate homologs of the Drosophila brain-specific
RT homeobox gene.";
RL Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, MUTAGENESIS OF
RP ARG-109 AND ARG-111, AND SUBCELLULAR LOCATION.
RX PubMed=17353277; DOI=10.1128/mcb.01561-06;
RA Chu H.-Y., Ohtoshi A.;
RT "Cloning and functional analysis of hypothalamic homeobox gene Bsx1a and
RT its isoform, Bsx1b.";
RL Mol. Cell. Biol. 27:3743-3749(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=14678827; DOI=10.1016/s1567-133x(03)00151-0;
RA Cremona M., Colombo E., Andreazzoli M., Cossu G., Broccoli V.;
RT "Bsx, an evolutionary conserved brain specific homeobox gene expressed in
RT the septum, epiphysis, mammillary bodies and arcuate nucleus.";
RL Gene Expr. Patterns 4:47-51(2004).
RN [5]
RP FUNCTION.
RX PubMed=17550780; DOI=10.1016/j.cmet.2007.05.007;
RA Sakkou M., Wiedmer P., Anlag K., Hamm A., Seuntjens E., Ettwiller L.,
RA Tschoep M.H., Treier M.;
RT "A role for brain-specific homeobox factor bsx in the control of
RT hyperphagia and locomotory behavior.";
RL Cell Metab. 5:450-463(2007).
RN [6]
RP FUNCTION.
RX PubMed=17485440; DOI=10.1128/mcb.00215-07;
RA McArthur T., Ohtoshi A.;
RT "A brain-specific homeobox gene, bsx, is essential for proper postnatal
RT growth and nursing.";
RL Mol. Cell. Biol. 27:5120-5127(2007).
CC -!- FUNCTION: DNA binding protein that function as transcriptional
CC activator. Is essential for normal postnatal growth and nursing. Is an
CC essential factor for neuronal neuropeptide Y and agouti-related peptide
CC function and locomotory behavior in the control of energy balance.
CC {ECO:0000269|PubMed:17353277, ECO:0000269|PubMed:17485440,
CC ECO:0000269|PubMed:17550780}.
CC -!- SUBCELLULAR LOCATION: [Isoform 1]: Nucleus
CC {ECO:0000269|PubMed:17353277}.
CC -!- SUBCELLULAR LOCATION: [Isoform 2]: Cytoplasm
CC {ECO:0000269|PubMed:17353277}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1; Synonyms=Bsxla;
CC IsoId=Q810B3-1; Sequence=Displayed;
CC Name=2; Synonyms=Bsxlb;
CC IsoId=Q810B3-2; Sequence=VSP_027570, VSP_027571;
CC -!- TISSUE SPECIFICITY: Expressed in brain. In brain, it is restricted to a
CC few specific developing brain structures such as pineal gland,
CC telencephalic septum, hypothalamic pre-mammillary body and arcuate
CC nucleus. {ECO:0000269|PubMed:14678827}.
CC -!- DEVELOPMENTAL STAGE: Expressed in early embryonic stages of epiphysis
CC development from 9.5 dpc onwards. {ECO:0000269|PubMed:14678827}.
CC -!- SIMILARITY: Belongs to the distal-less homeobox family. {ECO:0000255}.
CC -!- WEB RESOURCE: Name=Protein Spotlight; Note=Of fidgets and food - Issue
CC 85 of August 2007;
CC URL="https://web.expasy.org/spotlight/back_issues/085";
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AY233396; AAO84023.1; -; mRNA.
DR EMBL; BC104386; AAI04387.1; -; mRNA.
DR EMBL; BC104387; AAI04388.1; -; mRNA.
DR CCDS; CCDS23084.1; -. [Q810B3-1]
DR RefSeq; NP_839976.1; NM_178245.3. [Q810B3-1]
DR AlphaFoldDB; Q810B3; -.
DR SMR; Q810B3; -.
DR BioGRID; 232691; 2.
DR STRING; 10090.ENSMUSP00000068057; -.
DR PhosphoSitePlus; Q810B3; -.
DR PaxDb; Q810B3; -.
DR PRIDE; Q810B3; -.
DR Antibodypedia; 9208; 105 antibodies from 19 providers.
DR DNASU; 244813; -.
DR Ensembl; ENSMUST00000067375; ENSMUSP00000068057; ENSMUSG00000054360. [Q810B3-1]
DR GeneID; 244813; -.
DR KEGG; mmu:244813; -.
DR UCSC; uc009paa.1; mouse. [Q810B3-1]
DR CTD; 390259; -.
DR MGI; MGI:2669849; Bsx.
DR VEuPathDB; HostDB:ENSMUSG00000054360; -.
DR eggNOG; KOG0491; Eukaryota.
DR GeneTree; ENSGT00940000161473; -.
DR HOGENOM; CLU_104234_0_0_1; -.
DR InParanoid; Q810B3; -.
DR OMA; DLPGKHC; -.
DR OrthoDB; 1572711at2759; -.
DR PhylomeDB; Q810B3; -.
DR TreeFam; TF350735; -.
DR BioGRID-ORCS; 244813; 4 hits in 73 CRISPR screens.
DR PRO; PR:Q810B3; -.
DR Proteomes; UP000000589; Chromosome 9.
DR RNAct; Q810B3; protein.
DR Bgee; ENSMUSG00000054360; Expressed in ureteric bud trunk and 16 other tissues.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IDA:MGI.
DR GO; GO:0005667; C:transcription regulator complex; IDA:MGI.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IDA:NTNU_SB.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IDA:NTNU_SB.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:MGI.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:MGI.
DR GO; GO:0007420; P:brain development; ISS:UniProtKB.
DR GO; GO:0042755; P:eating behavior; IMP:MGI.
DR GO; GO:0007626; P:locomotory behavior; IMP:MGI.
DR GO; GO:0060056; P:mammary gland involution; IMP:MGI.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:NTNU_SB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR020479; Homeobox_metazoa.
DR Pfam; PF00046; Homeodomain; 1.
DR PRINTS; PR00024; HOMEOBOX.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 1: Evidence at protein level;
KW Activator; Alternative splicing; Cytoplasm; DNA-binding; Homeobox; Nucleus;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..232
FT /note="Brain-specific homeobox protein homolog"
FT /id="PRO_0000048844"
FT DNA_BIND 108..168
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 160..232
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 88..131
FT /note="GMPVPALFPHPQHAELPGKHCRRRKARTVFSDSQLSGLEKRFEI -> ALRP
FT GEKVRNPALPVDARACGAHCPQPLRDSGENVVPEPADEA (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:17353277"
FT /id="VSP_027570"
FT VAR_SEQ 132..232
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:17353277"
FT /id="VSP_027571"
FT MUTAGEN 109
FT /note="R->G: Abolishes the nuclear localization; when
FT associated with G-101."
FT /evidence="ECO:0000269|PubMed:17353277"
FT MUTAGEN 111
FT /note="R->G: Abolishes the nuclear localization; when
FT associated with G-109."
FT /evidence="ECO:0000269|PubMed:17353277"
SQ SEQUENCE 232 AA; 25862 MW; FEE7C9F1574F5624 CRC64;
MNLNFTSPLH PASSQRPTSF FIEDILLHKP KPLREVAPDH FASSLASRVP LLDYGYPLMP
TPTLLTPHAH HPLHKGDHHH PYFLTTSGMP VPALFPHPQH AELPGKHCRR RKARTVFSDS
QLSGLEKRFE IQRYLSTPER VELATALSLS ETQVKTWFQN RRMKHKKQLR KSQDEPKAAD
GPESPEGSPR APEGAPADAR LSLPAGAFVL TEPEDEVDIG DEGELSSGPH VL
