TINC_DROME
ID TINC_DROME Reviewed; 1513 AA.
AC Q86B91; A4V318; Q1EBX7; Q1EBX9; Q6NP49; Q86B90; Q8MUW7; Q8MZ15; Q9VEE7;
DT 22-FEB-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=Protein tincar;
GN Name=tinc; ORFNames=CG31247;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1] {ECO:0000305, ECO:0000312|EMBL:AAM43929.1}
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B), SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC STRAIN=Canton-S {ECO:0000269|PubMed:14516698};
RC TISSUE=Embryo {ECO:0000269|PubMed:14516698};
RX PubMed=14516698; DOI=10.1016/s0925-4773(03)00129-1;
RA Hirota Y., Sawamoto K., Okano H.;
RT "tincar encodes a novel transmembrane protein expressed in the Tinman-
RT expressing cardioblasts of Drosophila.";
RL Mech. Dev. 119:S279-S283(2002).
RN [2] {ECO:0000312|EMBL:AAO41572.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAO41572.1};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3] {ECO:0000305, ECO:0000312|EMBL:AAO41572.1}
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley {ECO:0000269|PubMed:12537572};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4] {ECO:0000305, ECO:0000312|EMBL:AAR82748.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-207 (ISOFORMS A/B), AND
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1046-1513 (ISOFORM A).
RC STRAIN=Berkeley {ECO:0000312|EMBL:ABG02201.1};
RA Stapleton M., Agbayani A., Brokstein P., Carlson J., Champe M., Chavez C.,
RA Dorsett V., Dresnek D., Farfan D., Frise E., George R., Gonzalez M.,
RA Guarin H., Hong L., Kronmiller B., Lewis S.E., Li P., Liao G., Miranda A.,
RA Mungall C.J., Nunoo J., Pacleb J., Paragas V., Park S., Patel S.,
RA Phouanenavong S., Rubin G.M., Wan K., Yu C., Celniker S.;
RL Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases.
RN [5] {ECO:0000305, ECO:0000312|EMBL:AAM29425.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 578-986 (ISOFORM A).
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAM29425.1};
RC TISSUE=Embryo {ECO:0000312|EMBL:AAM29425.1};
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [6] {ECO:0000305}
RP FUNCTION, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC STRAIN=Canton-S {ECO:0000269|PubMed:15654626};
RX PubMed=15654626; DOI=10.1007/s00427-004-0452-y;
RA Hirota Y., Sawamoto K., Takahashi K., Ueda R., Okano H.;
RT "The transmembrane protein, Tincar, is involved in the development of the
RT compound eye in Drosophila melanogaster.";
RL Dev. Genes Evol. 215:90-96(2005).
CC -!- FUNCTION: Involved in eye morphogenesis. May be essential for the
CC normal differentiation of ommatidial cells.
CC {ECO:0000269|PubMed:15654626}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000269|PubMed:14516698,
CC ECO:0000305}; Multi-pass membrane protein {ECO:0000269|PubMed:14516698,
CC ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=A {ECO:0000269|PubMed:10731132}; Synonyms=E
CC {ECO:0000312|FlyBase:FBgn0261649}, F {ECO:0000312|FlyBase:FBgn0261649},
CC G {ECO:0000312|FlyBase:FBgn0261649};
CC IsoId=Q86B91-1; Sequence=Displayed;
CC Name=B {ECO:0000269|PubMed:14516698};
CC IsoId=Q86B91-2; Sequence=VSP_042372, VSP_042375;
CC -!- TISSUE SPECIFICITY: Expression varies in tissues throughout
CC development. At stage 5, expressed in the embryo dorsal region followed
CC by expression in a striped pattern at stage 6. During gastrulation,
CC expressed in ventral region and ventral nerve cord. Also detected in
CC many neurons in the externa sensilla and chordotonal organ. At stage
CC 16, expressed on the surface of the midgut. Additionally, expressed in
CC a subset of cardioblasts (Tin+ subpopulation) during dorsal vessel
CC formation. In third-instar larval tissues, expressed in the eye and
CC antennal disks. In the antennal disks, expressed in the second antennal
CC segments. In the eye disks, strongest expression found in the ocelli,
CC and in the differentiating ommatidial cells. Also expressed in all
CC cells within and in the vicinity of the morphogenetic furrow.
CC {ECO:0000269|PubMed:14516698, ECO:0000269|PubMed:15654626}.
CC -!- DEVELOPMENTAL STAGE: Expressed in embryo from stage-5 onwards.
CC Expressed in larvae. {ECO:0000269|PubMed:14516698,
CC ECO:0000269|PubMed:15654626}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAM29425.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=ABG02199.1; Type=Miscellaneous discrepancy; Note=Sequence of unknown origin in the N-terminal part.; Evidence={ECO:0000305};
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DR EMBL; AF509933; AAM43929.1; -; mRNA.
DR EMBL; AE014297; AAF55477.3; -; Genomic_DNA.
DR EMBL; AE014297; AAO41572.1; -; Genomic_DNA.
DR EMBL; AE014297; AAO41573.3; -; Genomic_DNA.
DR EMBL; AE014297; AAO41574.2; -; Genomic_DNA.
DR EMBL; BT011082; AAR82748.1; -; mRNA.
DR EMBL; BT025955; ABG02199.1; ALT_SEQ; mRNA.
DR EMBL; BT025957; ABG02201.1; -; mRNA.
DR EMBL; AY113420; AAM29425.1; ALT_INIT; mRNA.
DR RefSeq; NP_001262686.1; NM_001275757.1. [Q86B91-1]
DR RefSeq; NP_732278.2; NM_169780.5. [Q86B91-2]
DR RefSeq; NP_788686.1; NM_176509.4. [Q86B91-1]
DR RefSeq; NP_788687.3; NM_176510.5. [Q86B91-1]
DR RefSeq; NP_788688.2; NM_176511.3. [Q86B91-1]
DR AlphaFoldDB; Q86B91; -.
DR SMR; Q86B91; -.
DR BioGRID; 67179; 17.
DR IntAct; Q86B91; 8.
DR STRING; 7227.FBpp0088712; -.
DR PaxDb; Q86B91; -.
DR PRIDE; Q86B91; -.
DR EnsemblMetazoa; FBtr0089771; FBpp0088712; FBgn0261649. [Q86B91-1]
DR EnsemblMetazoa; FBtr0089772; FBpp0088713; FBgn0261649. [Q86B91-2]
DR EnsemblMetazoa; FBtr0304905; FBpp0293444; FBgn0261649. [Q86B91-1]
DR EnsemblMetazoa; FBtr0337038; FBpp0307967; FBgn0261649. [Q86B91-1]
DR EnsemblMetazoa; FBtr0337039; FBpp0307968; FBgn0261649. [Q86B91-1]
DR GeneID; 42148; -.
DR KEGG; dme:Dmel_CG31247; -.
DR UCSC; CG31247-RA; d. melanogaster. [Q86B91-1]
DR UCSC; CG31247-RB; d. melanogaster.
DR UCSC; CG31247-RC; d. melanogaster.
DR CTD; 42148; -.
DR FlyBase; FBgn0261649; tinc.
DR VEuPathDB; VectorBase:FBgn0261649; -.
DR eggNOG; ENOG502QUZG; Eukaryota.
DR HOGENOM; CLU_246923_0_0_1; -.
DR InParanoid; Q86B91; -.
DR OMA; QSFAHGH; -.
DR PhylomeDB; Q86B91; -.
DR BioGRID-ORCS; 42148; 0 hits in 1 CRISPR screen.
DR ChiTaRS; tinc; fly.
DR GenomeRNAi; 42148; -.
DR PRO; PR:Q86B91; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0261649; Expressed in central nervous system and 14 other tissues.
DR ExpressionAtlas; Q86B91; baseline and differential.
DR Genevisible; Q86B91; DM.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0042478; P:regulation of eye photoreceptor cell development; IMP:FlyBase.
PE 2: Evidence at transcript level;
KW Alternative splicing; Developmental protein; Differentiation; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..1513
FT /note="Protein tincar"
FT /id="PRO_0000415800"
FT TOPO_DOM 1..77
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 78..98
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 99..120
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 121..141
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 142..181
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 182..202
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 203..668
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 669..689
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 690..696
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 697..717
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 718..736
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 737..757
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 758..787
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 788..808
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 809..826
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 827..847
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 848..1513
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 247..266
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 354..373
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 383..532
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 879..913
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1045..1090
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1115..1155
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1173..1214
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1231..1335
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 251..266
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 393..474
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 479..503
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 504..532
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1058..1074
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1124..1149
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1183..1214
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1253..1283
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1301..1315
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1321..1335
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 968..992
FT /note="STSDDVLLIGDQEQTDGKRHTSRGA -> TIRAVEPRYVLPVLRGKLTTAST
FT RH (in isoform B)"
FT /evidence="ECO:0000303|PubMed:14516698"
FT /id="VSP_042372"
FT VAR_SEQ 993..1513
FT /note="Missing (in isoform B)"
FT /evidence="ECO:0000303|PubMed:14516698"
FT /id="VSP_042375"
FT CONFLICT 922
FT /note="M -> I (in Ref. 5; AAM29425)"
FT /evidence="ECO:0000305"
FT CONFLICT 1314
FT /note="Q -> H (in Ref. 4; AAR82748)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1513 AA; 163381 MW; 229E41B667DBC037 CRC64;
MGGKHQGSGA GASSGGGSLN SSLCNSVMTN ATTASSSLTQ QQQQLQAKYI KSKRHQSRYT
SLQHSGHDSG SYLHLNSLWS IWYGVMLTLF QGYLAMHGAY RFLGCSLIPW KIEPVAELNL
QIVLSGVVFI LLPVFFTSAV FKVGNLANDG IKLATGARER RCTLSPHDGL EEESRGGTLR
ALWTHGGPTA AFVHIVIALC LLLPRLLLEA RIIENGLLPK EQIWATELDF VVINRRNLMA
MSVVGATPFP RHHQPSNQQQ SHHQHGRLNL TANSLEQDEE DYFNDTMFTA IRGVPAGGNN
NLFDLRPDRM ANGRKAAKTT ATTRTTTTAN KLDAGKGQYF EVPDLINQDI DEEERQELEE
AIRGEEDDGD EGVGVGEEGT VIMPDFDELA PRTAVGTSTT TAKASSDADK LNVENWQQLG
TLGKADASSS SSSTTSTTTT TTTSTTTTAA TTTSTRGTST TTTTTTIKPM EITTSRQSAH
HHHGKSRKHH KHHNKQRQQQ PPRRHHVASH EQAILESFPE EETTTRDSSI HRVRPEVLPE
LPIPSTPVSA SNSKIIAIKA KNQKRRISKR AAGVEIEPEY LLGDANINSS EFVAKSNEEE
PEESEDFELE DGDFQAVPAL TPAPPAEPNG PKSGQDYVRL DGFAGMLQLF FGIDKPIDVA
IFSQPPSAEF VNLLCALLVW SVRYPAVFWN TSKAFACVFS LQMVVAALDI ILGYVGISNL
YKLQIYAEAM PVHQPGLILN AVVTLALYLL STALVLASSM VMYLYGHGRL ATRMRDRSII
TLKTHQTWIY FAHCASLCFV LALAVVKAPL LNDLSATYKN NLHCPTFLAA LVGVTHLLLW
IVIWLCLTIK RRWHFKLPPL DSTYGGLLGK SSAQPLLMSS GQRTGSNSSS SGCNSTSTTV
NGGDSKPDMM STATSTELGM GMGMGIGGGI NGGMGGTGMG LAAQEDIYWP KLTPSSPKLK
VTFNEVTSTS DDVLLIGDQE QTDGKRHTSR GASVCFASAT GEVDDGEYAT LRAATAGAVV
GITMGSMKRG LSNTSVGVSL LHLSEYDELP PPPPANLHHQ QQQQQQQQQR QAHSFAHGHP
HDYANLSGLG GISDDNISEE GKLLACVRDD SITYASTSDL EPPQPSAQAP PPPPPLPIKG
APVPQPPAVM PHSAGIYGRA PQAMPEMMQL SPEHHHNPLQ HSLQHPQHHP LQQQQQKQQT
PQHPLQQQGN PHQHLVSPLA PVTVAVHTNE AHIASSSTPR CLRRADSGVP NEALTPRSDT
TSTTESTNTT SPPERAPSES SSGVHSGEER ELEVIIRPRA NSKPPPRPPQ PPIQEEPYGR
CTNMRMSSFN ADPAATSSAV INSATLPPQR SVPEQKFDYT AHCSTMPLPV GCHSQQLAGS
GNGYASTSAM TSSMGGGGMP PLPPSQVSSF MTPNSMHYAN ASVALGNVSG QQQPHTTLPN
GVRYSNPHFL RRLPHVTKAA ESPYGHLGYG AGHHAFAKLP HETHPTIPED RDSANYSMAS
DQDCGLYVTA QLH