TIN_DROME
ID TIN_DROME Reviewed; 416 AA.
AC P22711; Q6AWK6; Q6UKJ7; Q6UKK8; Q6UKL1; Q6UKL4; Q6UKL5; Q9TY85; Q9VDA7;
DT 01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT 15-MAR-2005, sequence version 2.
DT 03-AUG-2022, entry version 166.
DE RecName: Full=Muscle-specific homeobox protein tinman;
DE AltName: Full=Msh-2;
DE AltName: Full=NK-4;
GN Name=tin; Synonyms=msh2; ORFNames=CG7895;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=1982429; DOI=10.1242/dev.110.3.661;
RA Bodmer R., Jan L.Y., Jan Y.N.;
RT "A new homeobox-containing gene, msh-2, is transiently expressed early
RT during mesoderm formation of Drosophila.";
RL Development 110:661-669(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RA Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M.,
RA Park S., Wan K.H., Yu C., Rubin G.M., Celniker S.E.;
RL Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-252.
RC STRAIN=F-1461S, F-274F, F-357F, F-517F, F-517S, F-531F, F-611F, F-775F,
RC F-96S, S-114S, S-1224F, S-174F, S-255S, S-2588S, S-26F, S-377F, S-438S,
RC S-483F, S-501F, S-501S, S-510S, S-521F, S-521S, S-549S, S-565F, S-581F,
RC S-5F, S-968F, and US-255F;
RX PubMed=15126403; DOI=10.1534/genetics.166.4.1845;
RA Balakirev E.S., Ayala F.J.;
RT "Nucleotide variation in the tinman and bagpipe homeobox genes of
RT Drosophila melanogaster.";
RL Genetics 166:1845-1856(2004).
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 267-416.
RC STRAIN=Canton-S;
RX PubMed=2573058; DOI=10.1073/pnas.86.20.7716;
RA Kim Y., Nirenberg M.;
RT "Drosophila NK-homeobox genes.";
RL Proc. Natl. Acad. Sci. U.S.A. 86:7716-7720(1989).
RN [7]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=7915669; DOI=10.1242/dev.118.3.719;
RA Bodmer R.;
RT "The gene tinman is required for specification of the heart and visceral
RT muscles in Drosophila.";
RL Development 118:719-729(1993).
RN [8]
RP FUNCTION.
RX PubMed=32950464; DOI=10.1016/j.ydbio.2020.09.001;
RA Howard A.M., Milner H., Hupp M., Willett C., Palermino K., Nowak S.J.;
RT "Akirin is critical for early tinman induction and subsequent formation of
RT the heart in Drosophila melanogaster.";
RL Dev. Biol. 469:1-11(2021).
CC -!- FUNCTION: Required for the development of heart and visceral muscle;
CC for the formation of somatic muscles. Has a crucial function in the
CC early mesodermal subdivisions. {ECO:0000269|PubMed:1982429,
CC ECO:0000269|PubMed:32950464, ECO:0000269|PubMed:7915669}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- DEVELOPMENTAL STAGE: Is initially expressed throughout the presumptive
CC mesoderm and becomes restricted to cardiac and visceral muscle.
CC {ECO:0000269|PubMed:1982429}.
CC -!- DISRUPTION PHENOTYPE: Absence of cardiac and midgut visceral muscle,
CC and defects in a subset of dorsal body wall muscles.
CC {ECO:0000269|PubMed:7915669}.
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DR EMBL; X55192; CAA38978.1; -; mRNA.
DR EMBL; AE014297; AAF55890.1; -; Genomic_DNA.
DR EMBL; BT015242; AAT94471.1; -; mRNA.
DR EMBL; AY368079; AAQ75389.1; -; Genomic_DNA.
DR EMBL; AY368080; AAQ75390.1; -; Genomic_DNA.
DR EMBL; AY368081; AAQ75391.1; -; Genomic_DNA.
DR EMBL; AY368082; AAQ75392.1; -; Genomic_DNA.
DR EMBL; AY368083; AAQ75393.1; -; Genomic_DNA.
DR EMBL; AY368084; AAQ75394.1; -; Genomic_DNA.
DR EMBL; AY368085; AAQ75395.1; -; Genomic_DNA.
DR EMBL; AY368086; AAQ75396.1; -; Genomic_DNA.
DR EMBL; AY368087; AAQ75397.1; -; Genomic_DNA.
DR EMBL; AY368088; AAQ75398.1; -; Genomic_DNA.
DR EMBL; AY368089; AAQ75399.1; -; Genomic_DNA.
DR EMBL; AY368090; AAQ75400.1; -; Genomic_DNA.
DR EMBL; AY368091; AAQ75401.1; -; Genomic_DNA.
DR EMBL; AY368092; AAQ75402.1; -; Genomic_DNA.
DR EMBL; AY368093; AAQ75403.1; -; Genomic_DNA.
DR EMBL; AY368094; AAQ75404.1; -; Genomic_DNA.
DR EMBL; AY368095; AAQ75405.1; -; Genomic_DNA.
DR EMBL; AY368096; AAQ75406.1; -; Genomic_DNA.
DR EMBL; AY368097; AAQ75407.1; -; Genomic_DNA.
DR EMBL; AY368098; AAQ75408.1; -; Genomic_DNA.
DR EMBL; AY368099; AAQ75409.1; -; Genomic_DNA.
DR EMBL; AY368100; AAQ75410.1; -; Genomic_DNA.
DR EMBL; AY368101; AAQ75411.1; -; Genomic_DNA.
DR EMBL; AY368102; AAQ75412.1; -; Genomic_DNA.
DR EMBL; AY368103; AAQ75413.1; -; Genomic_DNA.
DR EMBL; AY368104; AAQ75414.1; -; Genomic_DNA.
DR EMBL; AY368105; AAQ75415.1; -; Genomic_DNA.
DR EMBL; AY368106; AAQ75416.1; -; Genomic_DNA.
DR EMBL; AY368107; AAQ75417.1; -; Genomic_DNA.
DR EMBL; M27292; AAA28619.1; -; Genomic_DNA.
DR PIR; A43561; A43561.
DR RefSeq; NP_524433.1; NM_079709.3.
DR AlphaFoldDB; P22711; -.
DR SMR; P22711; -.
DR BioGRID; 67502; 16.
DR IntAct; P22711; 8.
DR STRING; 7227.FBpp0083485; -.
DR PaxDb; P22711; -.
DR PRIDE; P22711; -.
DR DNASU; 42536; -.
DR EnsemblMetazoa; FBtr0084086; FBpp0083485; FBgn0004110.
DR GeneID; 42536; -.
DR KEGG; dme:Dmel_CG7895; -.
DR UCSC; CG7895-RA; d. melanogaster.
DR CTD; 42536; -.
DR FlyBase; FBgn0004110; tin.
DR VEuPathDB; VectorBase:FBgn0004110; -.
DR eggNOG; KOG0842; Eukaryota.
DR HOGENOM; CLU_662713_0_0_1; -.
DR InParanoid; P22711; -.
DR OMA; PAQKLCG; -.
DR OrthoDB; 858478at2759; -.
DR PhylomeDB; P22711; -.
DR SignaLink; P22711; -.
DR BioGRID-ORCS; 42536; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 42536; -.
DR PRO; PR:P22711; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0004110; Expressed in myoblast and 17 other tissues.
DR ExpressionAtlas; P22711; baseline and differential.
DR Genevisible; P22711; DM.
DR GO; GO:0005634; C:nucleus; IDA:FlyBase.
DR GO; GO:0003677; F:DNA binding; IDA:FlyBase.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IDA:FlyBase.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IDA:FlyBase.
DR GO; GO:0055007; P:cardiac muscle cell differentiation; IMP:FlyBase.
DR GO; GO:0010002; P:cardioblast differentiation; IMP:FlyBase.
DR GO; GO:0035051; P:cardiocyte differentiation; IMP:FlyBase.
DR GO; GO:0030154; P:cell differentiation; IBA:GO_Central.
DR GO; GO:0071907; P:determination of digestive tract left/right asymmetry; IMP:FlyBase.
DR GO; GO:0048615; P:embryonic anterior midgut (ectodermal) morphogenesis; IMP:FlyBase.
DR GO; GO:0035050; P:embryonic heart tube development; IMP:FlyBase.
DR GO; GO:0008354; P:germ cell migration; IMP:FlyBase.
DR GO; GO:0008406; P:gonad development; TAS:FlyBase.
DR GO; GO:0007506; P:gonadal mesoderm development; IMP:FlyBase.
DR GO; GO:0048542; P:lymph gland development; IMP:FlyBase.
DR GO; GO:0007498; P:mesoderm development; IEP:FlyBase.
DR GO; GO:0007552; P:metamorphosis; TAS:FlyBase.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; TAS:FlyBase.
DR GO; GO:0061101; P:neuroendocrine cell differentiation; IMP:FlyBase.
DR GO; GO:0061320; P:pericardial nephrocyte differentiation; IMP:FlyBase.
DR GO; GO:0007280; P:pole cell migration; TAS:FlyBase.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:FlyBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0007435; P:salivary gland morphogenesis; IMP:FlyBase.
DR GO; GO:0007419; P:ventral cord development; HMP:FlyBase.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR Pfam; PF00046; Homeodomain; 1.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 2: Evidence at transcript level;
KW Developmental protein; DNA-binding; Homeobox; Nucleus; Reference proteome.
FT CHAIN 1..416
FT /note="Muscle-specific homeobox protein tinman"
FT /id="PRO_0000049080"
FT DNA_BIND 301..360
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 1..33
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 246..305
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 391..416
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 259..273
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 274..297
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 393..416
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VARIANT 12
FT /note="G -> S (in strain: S-521F)"
FT VARIANT 71
FT /note="A -> G (in strain: F-96S, F-775F, S-26F, S-438S, S-
FT 483F, S-510S, S-521S, S-549S, S-565F, S-968F and US-255F)"
FT VARIANT 173
FT /note="Y -> N (in strain: S-5F)"
FT VARIANT 238
FT /note="P -> A (in strain: S-255S)"
FT CONFLICT 199
FT /note="G -> D (in Ref. 1)"
FT /evidence="ECO:0000305"
FT CONFLICT 342
FT /note="A -> T (in Ref. 4)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 416 AA; 46066 MW; F58A4F92324A7E2B CRC64;
MLQHHQQQAQ SGGYYDHYTQ SPSPGSLTNA DALNTTPFSV KDILNMVNQT EAYEGSYGHI
DGAATASALF AAGEYQNPHQ YLNHQQHQQS ELPIPQQQLH HQHLDDGATT SSSLSPLLPP
PPHQLYGGYQ DYGMPAHMFQ HHHGHPHQSF QHSASAYNMS ASQFYAGASA TAYQTPATYN
YNYAGSGEVY GGATPSAVGI KSEYIPTPYV TPSPTLDLNS SAEVDSLQAP TQKLCVNPLS
QRLMETASNS SSLRSIYGSD EGAKKKDNSQ VTSSRSELRK NSISGNSNPG SNSGSTKPRM
KRKPRVLFSQ AQVLELECRF RLKKYLTGAE REIIAQKLNL SATQVKIWFQ NRRYKSKRGD
IDCEGIAKHL KLKSEPLDSP TSLPPPIPNH VMWPPTMQQS QQQQQHHAQQ QQMQHM
