ID   TIP1_MEDSA              Reviewed;         249 AA.
AC   P42067; O22525;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   27-JUN-2003, sequence version 2.
DT   25-MAY-2022, entry version 90.
DE   RecName: Full=Probable aquaporin TIP-type;
DE   AltName: Full=Membrane channel protein 1;
DE   AltName: Full=MsMCP1;
GN   Name=MCP1;
OS   Medicago sativa (Alfalfa).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; Hologalegina; IRL clade; Trifolieae; Medicago.
OX   NCBI_TaxID=3879;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Saranac; TISSUE=Root nodule;
RA   Gregerson R.G., Gantt J.S., Vance C.P.;
RT   "cDNA cloning and nucleotide sequence of an aquaporin homolog from
RT   Alfalfa.";
RL   (er) Plant Gene Register PGR98-029(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 1-204.
RC   STRAIN=cv. Saranac; TISSUE=Root nodule;
RA   Gregerson R.G., Gantt J.S.T., Vance C.P.;
RT   "Characterization of an alfalfa membrane channel protein cDNA.";
RL   Submitted (OCT-1994) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Aquaporins facilitate the transport of water and small
CC       neutral solutes across cell membranes. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Expression is highest in root tips, with slightly
CC       lower levels of hybridizing mRNA in stems, and whole roots, and much
CC       lower levels in nodules and leaves.
CC   -!- DOMAIN: Aquaporins contain two tandem repeats each containing three
CC       membrane-spanning domains and a pore-forming loop with the signature
CC       motif Asn-Pro-Ala (NPA).
CC   -!- SIMILARITY: Belongs to the MIP/aquaporin (TC 1.A.8) family. TIP (TC
CC       1.A.8.10) subfamily. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAB41809.1; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; AF020793; AAC04846.1; -; mRNA.
DR   EMBL; L36881; AAB41809.1; ALT_FRAME; mRNA.
DR   PIR; T09297; T09297.
DR   PIR; T09621; T09621.
DR   AlphaFoldDB; P42067; -.
DR   SMR; P42067; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0015267; F:channel activity; IEA:InterPro.
DR   CDD; cd00333; MIP; 1.
DR   Gene3D; 1.20.1080.10; -; 1.
DR   InterPro; IPR023271; Aquaporin-like.
DR   InterPro; IPR034294; Aquaporin_transptr.
DR   InterPro; IPR000425; MIP.
DR   PANTHER; PTHR45665; PTHR45665; 1.
DR   Pfam; PF00230; MIP; 1.
DR   PRINTS; PR00783; MINTRINSICP.
DR   SUPFAM; SSF81338; SSF81338; 1.
PE   2: Evidence at transcript level;
KW   Membrane; Repeat; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..249
FT                   /note="Probable aquaporin TIP-type"
FT                   /id="PRO_0000064019"
FT   TRANSMEM        22..42
FT                   /note="Helical; Name=1"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        56..76
FT                   /note="Helical; Name=2"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        103..123
FT                   /note="Helical; Name=3"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        137..157
FT                   /note="Helical; Name=4"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        169..189
FT                   /note="Helical; Name=5"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        217..237
FT                   /note="Helical; Name=6"
FT                   /evidence="ECO:0000255"
FT   MOTIF           85..87
FT                   /note="NPA 1"
FT   MOTIF           197..199
FT                   /note="NPA 2"
SQ   SEQUENCE   249 AA;  25324 MW;  84C443202DCD025B CRC64;
     MPIRNIAVGT PQEATHPDTL KAGLAEFIST FIFVFAGSGS GIAYNKLTND GAATPAGLIS
     ASIAHAFALF VAVSVGANIS GGHVNPAVFG AFVGGNITLL RGIVYIIAQL LGSIVCSALL
     VFVTASSVPA FGLSEGVGVG PALVLEIVMT FGLVYTVYAT AVDPKKGNIG IIAPIAIGFI
     VGANILVGGA FTGASMNPAV SFGPAVVSWS WSNHWVYWAG PLIGGGIAGL VYEVLFINST
     HEQLPTTDY