ID   TIP1_MEDTR              Reviewed;         250 AA.
AC   Q9FY14;
DT   27-JUN-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   25-MAY-2022, entry version 79.
DE   RecName: Full=Probable aquaporin TIP-type;
DE   AltName: Full=MtAQP1;
GN   Name=AQP1;
OS   Medicago truncatula (Barrel medic) (Medicago tribuloides).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; Hologalegina; IRL clade; Trifolieae; Medicago.
OX   NCBI_TaxID=3880;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND CHARACTERIZATION.
RC   STRAIN=cv. Jemalong; TISSUE=Arbuscular mycorrhiza;
RX   PubMed=10923707; DOI=10.1007/s004250000263;
RA   Krajinski F., Biela A., Schubert D., Gianinazzi-Pearson V., Kaldenhoff R.,
RA   Franken P.;
RT   "Arbuscular mycorrhiza development regulates the mRNA abundance of Mtaqp1
RT   encoding a mercury-insensitive aquaporin of Medicago truncatula.";
RL   Planta 211:85-90(2000).
CC   -!- FUNCTION: Aquaporins facilitate the transport of water and small
CC       neutral solutes across cell membranes. May have a role in buffering
CC       osmotic fluctations in the highly compartmented vacuole of arbuscule
CC       cells.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- DOMAIN: Aquaporins contain two tandem repeats each containing three
CC       membrane-spanning domains and a pore-forming loop with the signature
CC       motif Asn-Pro-Ala (NPA).
CC   -!- SIMILARITY: Belongs to the MIP/aquaporin (TC 1.A.8) family. TIP (TC
CC       1.A.8.10) subfamily. {ECO:0000305}.
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DR   EMBL; AJ251652; CAC01618.1; -; mRNA.
DR   RefSeq; XP_013450030.1; XM_013594576.1.
DR   AlphaFoldDB; Q9FY14; -.
DR   SMR; Q9FY14; -.
DR   EnsemblPlants; KEH24058; KEH24058; MTR_7g103030.
DR   GeneID; 25499345; -.
DR   Gramene; KEH24058; KEH24058; MTR_7g103030.
DR   HOGENOM; CLU_020019_3_4_1; -.
DR   OrthoDB; 1152704at2759; -.
DR   ExpressionAtlas; Q9FY14; differential.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0015267; F:channel activity; IEA:InterPro.
DR   CDD; cd00333; MIP; 1.
DR   Gene3D; 1.20.1080.10; -; 1.
DR   InterPro; IPR023271; Aquaporin-like.
DR   InterPro; IPR034294; Aquaporin_transptr.
DR   InterPro; IPR000425; MIP.
DR   InterPro; IPR022357; MIP_CS.
DR   PANTHER; PTHR45665; PTHR45665; 1.
DR   Pfam; PF00230; MIP; 1.
DR   PRINTS; PR00783; MINTRINSICP.
DR   SUPFAM; SSF81338; SSF81338; 1.
DR   PROSITE; PS00221; MIP; 1.
PE   1: Evidence at protein level;
KW   Membrane; Repeat; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..250
FT                   /note="Probable aquaporin TIP-type"
FT                   /id="PRO_0000064020"
FT   TRANSMEM        22..42
FT                   /note="Helical; Name=1"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        56..76
FT                   /note="Helical; Name=2"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        104..124
FT                   /note="Helical; Name=3"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        138..158
FT                   /note="Helical; Name=4"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        170..190
FT                   /note="Helical; Name=5"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        218..238
FT                   /note="Helical; Name=6"
FT                   /evidence="ECO:0000255"
FT   MOTIF           85..87
FT                   /note="NPA 1"
FT   MOTIF           198..200
FT                   /note="NPA 2"
SQ   SEQUENCE   250 AA;  25393 MW;  A140AADA123F535D CRC64;
     MPIRNIAVGT PQEATHPDTL KAGLAEFIST FIFVFAGSGS GIAYNKLTND GAATPAGLIS
     ASIAHAFALF VAVSVGANIS GGHVNPAVTF GAFVGGNITL LRGIVYIIAQ LLGSIVASAL
     LVFVTASSVP AFGLSEGVGV GPALVLEIVM TFGLVYTVYA TAVDPKKGNI GIIAPIAIGF
     IVGANILVGG AFTGASMNPA VSFGPAVVSW SWSNHWVYWA GPLIGGGIAG LVYEVLFINS
     THEQLPTTDY