ID   TIP1_TOBAC              Reviewed;         250 AA.
AC   P21653;
DT   01-MAY-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1991, sequence version 1.
DT   03-AUG-2022, entry version 115.
DE   RecName: Full=Probable aquaporin TIP-type RB7-5A;
DE   AltName: Full=RT-TIP;
DE   AltName: Full=TobRB7;
DE   AltName: Full=Tonoplast intrinsic protein, root-specific RB7-5A;
OS   Nicotiana tabacum (Common tobacco).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC   Nicotiana.
OX   NCBI_TaxID=4097;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1840917; DOI=10.2307/3869212;
RA   Yamamoto Y.T., Taylor C.G., Acedo G.N., Cheng C.-L., Conkling M.A.;
RT   "Characterization of cis-acting sequences regulating root-specific gene
RT   expression in tobacco.";
RL   Plant Cell 3:371-382(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Wisconsin 38; TISSUE=Root;
RX   PubMed=2129561; DOI=10.1093/nar/18.24.7449;
RA   Yamamoto Y.T., Cheng C.-L., Conkling M.A.;
RT   "Root-specific genes from tobacco and Arabidopsis homologous to an
RT   evolutionarily conserved gene family of membrane channel proteins.";
RL   Nucleic Acids Res. 18:7449-7449(1990).
CC   -!- FUNCTION: Channel protein in tonoplast. These proteins may allow the
CC       diffusion of amino acids and/or peptides from the vacuolar compartment
CC       to the cytoplasm.
CC   -!- SUBCELLULAR LOCATION: Vacuole membrane; Multi-pass membrane protein.
CC       Note=Tonoplast.
CC   -!- TISSUE SPECIFICITY: Roots.
CC   -!- DOMAIN: Aquaporins contain two tandem repeats each containing three
CC       membrane-spanning domains and a pore-forming loop with the signature
CC       motif Asn-Pro-Ala (NPA).
CC   -!- SIMILARITY: Belongs to the MIP/aquaporin (TC 1.A.8) family. TIP (TC
CC       1.A.8.10) subfamily. {ECO:0000305}.
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DR   EMBL; S45406; AAB23597.2; -; Genomic_DNA.
DR   EMBL; X54855; CAA38634.1; -; mRNA.
DR   PIR; S13719; S13719.
DR   RefSeq; NP_001312940.1; NM_001326011.1.
DR   AlphaFoldDB; P21653; -.
DR   SMR; P21653; -.
DR   STRING; 4097.P21653; -.
DR   ProMEX; P21653; -.
DR   GeneID; 107817944; -.
DR   KEGG; nta:107817944; -.
DR   OMA; YILFPQS; -.
DR   PhylomeDB; P21653; -.
DR   Proteomes; UP000084051; Unplaced.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0016020; C:membrane; IBA:GO_Central.
DR   GO; GO:0009705; C:plant-type vacuole membrane; IBA:GO_Central.
DR   GO; GO:0015250; F:water channel activity; IBA:GO_Central.
DR   GO; GO:0006833; P:water transport; IBA:GO_Central.
DR   CDD; cd00333; MIP; 1.
DR   Gene3D; 1.20.1080.10; -; 1.
DR   InterPro; IPR023271; Aquaporin-like.
DR   InterPro; IPR034294; Aquaporin_transptr.
DR   InterPro; IPR000425; MIP.
DR   InterPro; IPR022357; MIP_CS.
DR   PANTHER; PTHR45665; PTHR45665; 1.
DR   Pfam; PF00230; MIP; 1.
DR   PRINTS; PR00783; MINTRINSICP.
DR   SUPFAM; SSF81338; SSF81338; 1.
DR   TIGRFAMs; TIGR00861; MIP; 1.
DR   PROSITE; PS00221; MIP; 1.
PE   2: Evidence at transcript level;
KW   Membrane; Reference proteome; Repeat; Transmembrane; Transmembrane helix;
KW   Transport; Vacuole.
FT   CHAIN           1..250
FT                   /note="Probable aquaporin TIP-type RB7-5A"
FT                   /id="PRO_0000064043"
FT   TRANSMEM        20..42
FT                   /note="Helical; Name=1"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        55..77
FT                   /note="Helical; Name=2"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        97..119
FT                   /note="Helical; Name=3"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        140..162
FT                   /note="Helical; Name=4"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        172..194
FT                   /note="Helical; Name=5"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        215..237
FT                   /note="Helical; Name=6"
FT                   /evidence="ECO:0000255"
FT   MOTIF           83..85
FT                   /note="NPA 1"
FT   MOTIF           197..199
FT                   /note="NPA 2"
SQ   SEQUENCE   250 AA;  25233 MW;  6B243E8EB1DB0C1D CRC64;
     MVRIAFGSIG DSFSVGSLKA YVAEFIATLL FVFAGVGSAI AYNKLTADAA LDPAGLVAVA
     VAHAFALFVG VSIAANISGG HLNPAVTLGL AVGGNITILT GFFYWIAQLL GSTVACLLLK
     YVTNGLAVPT HGVAAGLNGL QGVVMEIIIT FALVYTVYAT AADPKKGSLG TIAPIAIGFI
     VGANILAAGP FSGGSMNPAR SFGPAVVAGD FSQNWIYWAG PLIGGGLAGF IYGDVFIGCH
     TPLPTSEDYA