TIP1_YEAST
ID TIP1_YEAST Reviewed; 210 AA.
AC P27654; D6VQ66;
DT 01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1992, sequence version 1.
DT 03-AUG-2022, entry version 169.
DE RecName: Full=Temperature shock-inducible protein 1;
DE EC=3.1.1.-;
DE Flags: Precursor;
GN Name=TIP1; OrderedLocusNames=YBR067C; ORFNames=YBR0622;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1894636; DOI=10.1016/s0021-9258(19)47405-x;
RA Kondo K., Inouye M.;
RT "TIP 1, a cold shock-inducible gene of Saccharomyces cerevisiae.";
RL J. Biol. Chem. 266:17537-17544(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7813418; DOI=10.1002/j.1460-2075.1994.tb06923.x;
RA Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C.,
RA Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M.,
RA Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M.,
RA Cziepluch C., Demolis N., Delaveau T., Doignon F., Domdey H.,
RA Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D.,
RA Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N.,
RA Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J.,
RA Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C.,
RA Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P.,
RA Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y.,
RA Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F.,
RA Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E.,
RA Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M.,
RA Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B.,
RA Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L.,
RA Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M.,
RA Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S.,
RA Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K.,
RA Mewes H.-W., Kleine K.;
RT "Complete DNA sequence of yeast chromosome II.";
RL EMBO J. 13:5795-5809(1994).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP PROTEIN SEQUENCE OF 20-48, GLYCOSYLATION, AND INDUCTION.
RX PubMed=7746155; DOI=10.1111/j.1365-2958.1995.tb02248.x;
RA Kowalski L.R.Z., Kondo K., Inouye M.;
RT "Cold-shock induction of a family of TIP1-related proteins associated with
RT the membrane in Saccharomyces cerevisiae.";
RL Mol. Microbiol. 15:341-353(1995).
RN [5]
RP PROTEIN SEQUENCE OF 20-48 AND 90-100, PROTEIN SEQUENCE OF 130-148 AND
RP 164-186, AND GPI-ANCHOR AT ASN-186.
RX PubMed=10216230; DOI=10.1016/s0304-4165(99)00012-4;
RA Fujii T., Shimoi H., Iimura Y.;
RT "Structure of the glucan-binding sugar chain of Tip1p, a cell wall protein
RT of Saccharomyces cerevisiae.";
RL Biochim. Biophys. Acta 1427:133-144(1999).
RN [6]
RP PROTEIN SEQUENCE OF 20-34, GLYCOSYLATION, AND SUBCELLULAR LOCATION.
RX PubMed=7768807; DOI=10.1128/jb.177.11.3104-3110.1995;
RA van der Vaart J.M., Caro L.H.P., Chapman J.W., Klis F.M., Verrips C.T.;
RT "Identification of three mannoproteins in the cell wall of Saccharomyces
RT cerevisiae.";
RL J. Bacteriol. 177:3104-3110(1995).
RN [7]
RP PROTEIN SEQUENCE OF 20-41, CHARACTERIZATION, AND MASS SPECTROMETRY.
RC STRAIN=Carlsbergensis / BK2539;
RX PubMed=9818717;
RX DOI=10.1002/(sici)1097-0061(19980630)14:9<793::aid-yea277>3.0.co;2-e;
RA Horsted M.W., Dey E.S., Holmberg S., Kielland-Brandt M.C.;
RT "A novel esterase from Saccharomyces carlsbergensis, a possible function
RT for the yeast TIP1 gene.";
RL Yeast 14:793-803(1998).
RN [8]
RP INDUCTION.
RX PubMed=11292809; DOI=10.1128/jb.183.9.2881-2887.2001;
RA Abramova N.E., Sertil O., Mehta S., Lowry C.V.;
RT "Reciprocal regulation of anaerobic and aerobic cell wall mannoprotein gene
RT expression in Saccharomyces cerevisiae.";
RL J. Bacteriol. 183:2881-2887(2001).
RN [9]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [10]
RP SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY, AND GPI-ANCHOR.
RX PubMed=15781460; DOI=10.1074/jbc.m500334200;
RA Yin Q.Y., de Groot P.W.J., Dekker H.L., de Jong L., Klis F.M.,
RA de Koster C.G.;
RT "Comprehensive proteomic analysis of Saccharomyces cerevisiae cell walls:
RT identification of proteins covalently attached via
RT glycosylphosphatidylinositol remnants or mild alkali-sensitive linkages.";
RL J. Biol. Chem. 280:20894-20901(2005).
RN [11]
RP SUBCELLULAR LOCATION.
RX PubMed=16672383; DOI=10.1091/mbc.e05-08-0738;
RA Smits G.J., Schenkman L.R., Brul S., Pringle J.R., Klis F.M.;
RT "Role of cell cycle-regulated expression in the localized incorporation of
RT cell wall proteins in yeast.";
RL Mol. Biol. Cell 17:3267-3280(2006).
CC -!- FUNCTION: Seems to have esterase activity. Prefers ester of fatty acids
CC from 4 to 16 carbon atoms.
CC -!- SUBCELLULAR LOCATION: Secreted, cell wall. Membrane; Lipid-anchor, GPI-
CC anchor. Note=Covalently-linked GPI-modified cell wall protein (GPI-CWP)
CC found only in mother cells.
CC -!- INDUCTION: Induced during anaerobic growth and by cold shock and heat
CC shock. {ECO:0000269|PubMed:11292809, ECO:0000269|PubMed:7746155}.
CC -!- PTM: Extensively O-glycosylated. {ECO:0000269|PubMed:7746155,
CC ECO:0000269|PubMed:7768807}.
CC -!- PTM: The GPI-anchor is attached to the protein in the endoplasmic
CC reticulum and serves to target the protein to the cell surface. There,
CC the glucosamine-inositol phospholipid moiety is cleaved off and the
CC GPI-modified mannoprotein is covalently attached via its lipidless GPI
CC glycan remnant to the 1,6-beta-glucan of the outer cell wall layer.
CC -!- MASS SPECTROMETRY: Mass=16944; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:9818717};
CC -!- MISCELLANEOUS: Present with 35600 molecules/cell in log phase SD
CC medium. {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the SRP1/TIP1 family. {ECO:0000305}.
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DR EMBL; M71216; AAA35157.1; -; Genomic_DNA.
DR EMBL; Z35936; CAA85011.1; -; Genomic_DNA.
DR EMBL; BK006936; DAA07186.1; -; Genomic_DNA.
DR PIR; A40979; A40979.
DR RefSeq; NP_009623.1; NM_001178415.1.
DR AlphaFoldDB; P27654; -.
DR BioGRID; 32770; 43.
DR DIP; DIP-4908N; -.
DR IntAct; P27654; 5.
DR MINT; P27654; -.
DR STRING; 4932.YBR067C; -.
DR PaxDb; P27654; -.
DR EnsemblFungi; YBR067C_mRNA; YBR067C; YBR067C.
DR GeneID; 852359; -.
DR KEGG; sce:YBR067C; -.
DR SGD; S000000271; TIP1.
DR VEuPathDB; FungiDB:YBR067C; -.
DR eggNOG; ENOG502RZ9R; Eukaryota.
DR GeneTree; ENSGT00940000176276; -.
DR HOGENOM; CLU_071083_1_1_1; -.
DR InParanoid; P27654; -.
DR OMA; ATELPWY; -.
DR BioCyc; YEAST:G3O-29036-MON; -.
DR PRO; PR:P27654; -.
DR Proteomes; UP000002311; Chromosome II.
DR RNAct; P27654; protein.
DR GO; GO:0031225; C:anchored component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0071944; C:cell periphery; HDA:SGD.
DR GO; GO:0005829; C:cytosol; HDA:SGD.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR GO; GO:0009277; C:fungal-type cell wall; IDA:SGD.
DR GO; GO:0016298; F:lipase activity; IDA:SGD.
DR GO; GO:0005199; F:structural constituent of cell wall; IDA:SGD.
DR GO; GO:0031505; P:fungal-type cell wall organization; IDA:SGD.
DR InterPro; IPR000992; SRP1_TIP1.
DR Pfam; PF00660; SRP1_TIP1; 1.
DR PROSITE; PS00724; SRP1_TIP1; 1.
PE 1: Evidence at protein level;
KW Cell wall; Direct protein sequencing; Glycoprotein; GPI-anchor; Hydrolase;
KW Lipoprotein; Membrane; Reference proteome; Secreted; Signal;
KW Stress response.
FT SIGNAL 1..19
FT /evidence="ECO:0000269|PubMed:10216230,
FT ECO:0000269|PubMed:7746155, ECO:0000269|PubMed:7768807,
FT ECO:0000269|PubMed:9818717"
FT CHAIN 20..186
FT /note="Temperature shock-inducible protein 1"
FT /id="PRO_0000033237"
FT PROPEP 187..210
FT /note="Removed in mature form"
FT /id="PRO_0000033238"
FT REGION 122..186
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT LIPID 186
FT /note="GPI-anchor amidated asparagine"
FT /evidence="ECO:0000269|PubMed:10216230"
SQ SEQUENCE 210 AA; 20728 MW; D607F34A9C1D108F CRC64;
MSVSKIAFVL SAIASLAVAD TSAAETAELQ AIIGDINSHL SDYLGLETGN SGFQIPSDVL
SVYQQVMTYT DDAYTTLFSE LDFDAITKTI VKLPWYTTRL SSEIAAALAS VSPASSEAAS
SSEAASSSKA ASSSEATSSA APSSSAAPSS SAAPSSSAES SSKAVSSSVA PTTSSVSTST
VETASNAGQR VNAGAASFGA VVAGAAALLL
