TIP2_ORYSJ
ID TIP2_ORYSJ Reviewed; 379 AA.
AC Q5JNS0; A0A0P0V176;
DT 09-JUL-2014, integrated into UniProtKB/Swiss-Prot.
DT 15-FEB-2005, sequence version 1.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=Transcription factor TIP2;
DE AltName: Full=Basic helix-loop-helix protein 142 {ECO:0000305};
DE Short=OsbHLH142 {ECO:0000303|PubMed:16896230};
DE AltName: Full=Basic helix-loop-helix protein TIP2;
DE AltName: Full=TDR INTERACTING PROTEIN2;
GN Name=TIP2; Synonyms=BHLH142 {ECO:0000305};
GN OrderedLocusNames=LOC_Os01g18870, Os01g0293100; ORFNames=P0706B05.43;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12447438; DOI=10.1038/nature01184;
RA Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y.,
RA Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H.,
RA Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.,
RA Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M.,
RA Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T.,
RA Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S.,
RA Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H.,
RA Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y.,
RA Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S.,
RA Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y.,
RA Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S.,
RA Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H.,
RA Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.;
RT "The genome sequence and structure of rice chromosome 1.";
RL Nature 420:312-316(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=16896230; DOI=10.1104/pp.106.080580;
RA Li X., Duan X., Jiang H., Sun Y., Tang Y., Yuan Z., Guo J., Liang W.,
RA Chen L., Yin J., Ma H., Wang J., Zhang D.;
RT "Genome-wide analysis of basic/helix-loop-helix transcription factor family
RT in rice and Arabidopsis.";
RL Plant Physiol. 141:1167-1184(2006).
RN [6]
RP FUNCTION, INTERACTION WITH TDR, SUBCELLULAR LOCATION, TISSUE SPECIFICITY,
RP AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. 9522;
RX PubMed=24755456; DOI=10.1105/tpc.114.123745;
RA Fu Z., Yu J., Cheng X., Zong X., Xu J., Chen M., Li Z., Zhang D., Liang W.;
RT "The rice basic helix-loop-helix transcription factor TDR INTERACTING
RT PROTEIN2 is a central switch in early anther development.";
RL Plant Cell 26:1512-1524(2014).
RN [7]
RP FUNCTION, AND INTERACTION WITH TDR.
RX PubMed=24894043; DOI=10.1105/tpc.114.126292;
RA Ko S.S., Li M.J., Sun-Ben Ku M., Ho Y.C., Lin Y.J., Chuang M.H.,
RA Hsing H.X., Lien Y.C., Yang H.T., Chang H.C., Chan M.T.;
RT "The bHLH142 transcription factor coordinates with TDR1 to modulate the
RT expression of EAT1 and regulate pollen development in rice.";
RL Plant Cell 26:2486-2504(2014).
CC -!- FUNCTION: Transcription factor that binds to the E-box-containing
CC promoter regions of the transcription factors TDR and EAT1, activating
CC their expression. May have a role in specifying the cell pattern of the
CC inner anther walls and functioning in meiosis progression. Required for
CC male reproduction (PubMed:24755456). Acts downstream of UDT1 and GAMYB,
CC but upstream of TDR1 and EAT1 in pollen development (PubMed:24894043).
CC {ECO:0000269|PubMed:24755456, ECO:0000269|PubMed:24894043}.
CC -!- SUBUNIT: Homodimer (By similarity). Interacts with TDR, but not with
CC EAT1 (PubMed:24755456, PubMed:24894043). {ECO:0000250,
CC ECO:0000269|PubMed:24755456, ECO:0000269|PubMed:24894043}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981,
CC ECO:0000269|PubMed:24755456}.
CC -!- TISSUE SPECIFICITY: Highly expressed in anthers; strong expression in
CC the middle layer and tapetum, and weak expression in the endothecium.
CC {ECO:0000269|PubMed:24755456}.
CC -!- DEVELOPMENTAL STAGE: In anthers, expressed from the meiosis stage to
CC mitosis, with maximum levels at stages 7 and 8, when meiosis initiates,
CC and when endothecium and the middle layer become condensed,
CC respectively.
CC -!- DISRUPTION PHENOTYPE: Has normal vegetative growth, inflorescence and
CC flower morphology, however has smaller anthers and does not produce
CC mature pollen grains during reproductive stage. After stage 6, during
CC which anther forms four somatic layers, the three inner anther wall
CC layers remain undifferentiated. Meiosis progresses normally from
CC leptotene stage to metaphase I, but is arrasted at anaphase I and no
CC cytokinesis occurs at prophase II, resulting in degenerated meiocytes.
CC {ECO:0000269|PubMed:24755456}.
CC -!- SIMILARITY: Belongs to the bHLH protein family.
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DR EMBL; AP002482; BAD86887.1; -; Genomic_DNA.
DR EMBL; AP008207; BAF04709.1; -; Genomic_DNA.
DR EMBL; AP014957; BAS71669.1; -; Genomic_DNA.
DR RefSeq; XP_015645943.1; XM_015790457.1.
DR AlphaFoldDB; Q5JNS0; -.
DR SMR; Q5JNS0; -.
DR STRING; 4530.OS01T0293100-01; -.
DR PaxDb; Q5JNS0; -.
DR PRIDE; Q5JNS0; -.
DR EnsemblPlants; Os01t0293100-01; Os01t0293100-01; Os01g0293100.
DR GeneID; 4325164; -.
DR Gramene; Os01t0293100-01; Os01t0293100-01; Os01g0293100.
DR KEGG; osa:4325164; -.
DR eggNOG; ENOG502SM54; Eukaryota.
DR HOGENOM; CLU_730377_0_0_1; -.
DR InParanoid; Q5JNS0; -.
DR OMA; QYHPATA; -.
DR OrthoDB; 948814at2759; -.
DR PlantReactome; R-OSA-8986768; Anther and pollen development.
DR Proteomes; UP000000763; Chromosome 1.
DR Proteomes; UP000059680; Chromosome 1.
DR Genevisible; Q5JNS0; OS.
DR GO; GO:0005634; C:nucleus; IC:Gramene.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0048658; P:anther wall tapetum development; IEA:InterPro.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IC:Gramene.
DR CDD; cd18918; bHLH_AtMYC1_like; 1.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR045895; bHLH91-like.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR InterPro; IPR045896; MYC1-like_bHLH.
DR PANTHER; PTHR46834; PTHR46834; 1.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..379
FT /note="Transcription factor TIP2"
FT /id="PRO_0000429745"
FT DOMAIN 173..222
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 144..184
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 173..186
FT /note="Basic motif; degenerate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 187..222
FT /note="Helix-loop-helix motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
SQ SEQUENCE 379 AA; 40706 MW; 88840ACC6D4353B5 CRC64;
MYHPQCELLM PLESLEMDVG QSHLAAAVAA AMPGELNFHL LHSLDAAAAA ASSTAASASS
QPTVDYFFGG ADQQPPPPAA MQYDQLAAPH HHQTVAMLRD YYGGHYPPAA AAAAATEAYF
RGGPRTAGSS SLVFGPADDE SAFMVGPFES SPTPRSGGGR KRSRATAGFH GGGPANGVEK
KEKQRRLRLT EKYNALMLLI PNRTKEDRAT VISDAIEYIQ ELGRTVEELT LLVEKKRRRR
EMQGDVVDAA TSSVVAGMDQ AAESSEGEVM AAAAMGAVAP PPRQAPIRST YIQRRSKETF
VDVRIVEDDV NIKLTKRRRD GCLAAASRAL DDLRLDLVHL SGGKIGDCHI YMFNTKIHSG
SPVFASAVAS RLIEVVDEY
