TIP2_TOBAC
ID TIP2_TOBAC Reviewed; 250 AA.
AC P24422;
DT 01-MAR-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-1993, sequence version 2.
DT 03-AUG-2022, entry version 101.
DE RecName: Full=Probable aquaporin TIP-type RB7-18C;
DE AltName: Full=RT-TIP;
DE AltName: Full=TobRB7;
DE AltName: Full=Tonoplast intrinsic protein, root-specific RB7-18C;
OS Nicotiana tabacum (Common tobacco).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC Nicotiana.
OX NCBI_TaxID=4097;
RN [1]
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Root;
RX PubMed=1840917; DOI=10.2307/3869212;
RA Yamamoto Y.T., Taylor C.G., Acedo G.N., Cheng C.-L., Conkling M.A.;
RT "Characterization of cis-acting sequences regulating root-specific gene
RT expression in tobacco.";
RL Plant Cell 3:371-382(1991).
CC -!- FUNCTION: Channel protein in tonoplast. These proteins may allow the
CC diffusion of amino acids and/or peptides from the vacuolar compartment
CC to the cytoplasm.
CC -!- SUBCELLULAR LOCATION: Vacuole membrane; Multi-pass membrane protein.
CC Note=Tonoplast.
CC -!- TISSUE SPECIFICITY: Roots.
CC -!- DOMAIN: Aquaporins contain two tandem repeats each containing three
CC membrane-spanning domains and a pore-forming loop with the signature
CC motif Asn-Pro-Ala (NPA).
CC -!- SIMILARITY: Belongs to the MIP/aquaporin (TC 1.A.8) family. TIP (TC
CC 1.A.8.10) subfamily. {ECO:0000305}.
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DR PIR; JQ1012; JQ1012.
DR AlphaFoldDB; P24422; -.
DR SMR; P24422; -.
DR STRING; 4097.P24422; -.
DR ProMEX; P24422; -.
DR Proteomes; UP000084051; Unplaced.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0009705; C:plant-type vacuole membrane; IBA:GO_Central.
DR GO; GO:0015250; F:water channel activity; IBA:GO_Central.
DR GO; GO:0006833; P:water transport; IBA:GO_Central.
DR CDD; cd00333; MIP; 1.
DR Gene3D; 1.20.1080.10; -; 1.
DR InterPro; IPR023271; Aquaporin-like.
DR InterPro; IPR034294; Aquaporin_transptr.
DR InterPro; IPR000425; MIP.
DR InterPro; IPR022357; MIP_CS.
DR PANTHER; PTHR45665; PTHR45665; 1.
DR Pfam; PF00230; MIP; 1.
DR PRINTS; PR00783; MINTRINSICP.
DR SUPFAM; SSF81338; SSF81338; 1.
DR TIGRFAMs; TIGR00861; MIP; 1.
DR PROSITE; PS00221; MIP; 1.
PE 2: Evidence at transcript level;
KW Membrane; Reference proteome; Repeat; Transmembrane; Transmembrane helix;
KW Transport; Vacuole.
FT CHAIN 1..250
FT /note="Probable aquaporin TIP-type RB7-18C"
FT /id="PRO_0000064044"
FT TRANSMEM 20..42
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 55..77
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TRANSMEM 97..119
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TRANSMEM 140..162
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TRANSMEM 172..194
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TRANSMEM 215..237
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT MOTIF 83..85
FT /note="NPA 1"
FT MOTIF 197..199
FT /note="NPA 2"
SQ SEQUENCE 250 AA; 25224 MW; 2BFF533547006438 CRC64;
MVLIAFGSIG DSFSVGSLKA YVAEFIATLL FVFAGVGSAI AYNKLTADAA LDPAGLVAVA
VAHAFALFVG VSIAANISGG HLNPAVTLGL AVGGNITILT GFFYWIAQLL GSTVACLLLK
YVTNGLAVPT HGVAAGLNGF QGVVMEIIIT FALVYTVYAT AADPKKGSLG TIAPIAIGFI
VGANILAAGP FSGGSMNPAR SFGPAVVAGD FSQNWIYWAG PLIGGGLAGF IYGDVFIGCH
TPLPTSEDYA