ID   TIP39_BOVIN             Reviewed;         100 AA.
AC   P0C171;
DT   21-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT   21-MAR-2006, sequence version 1.
DT   25-MAY-2022, entry version 39.
DE   RecName: Full=Tuberoinfundibular peptide of 39 residues;
DE            Short=TIP39;
DE   AltName: Full=Parathyroid hormone 2;
DE   Flags: Precursor;
GN   Name=PTH2; Synonyms=TIP39, TIPF39;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Hereford;
RX   PubMed=19390049; DOI=10.1126/science.1169588;
RG   The bovine genome sequencing and analysis consortium;
RT   "The genome sequence of taurine cattle: a window to ruminant biology and
RT   evolution.";
RL   Science 324:522-528(2009).
RN   [2]
RP   PROTEIN SEQUENCE OF 62-100, FUNCTION, INTERACTION WITH PTH2R, AND MASS
RP   SPECTROMETRY.
RC   TISSUE=Hypothalamus;
RX   PubMed=10526330; DOI=10.1038/14724;
RA   Usdin T.B., Hoare S.R.J., Wang T., Mezey E., Kowalak J.A.;
RT   "TIP39: a new neuropeptide and PTH2-receptor agonist from hypothalamus.";
RL   Nat. Neurosci. 2:941-943(1999).
RN   [3]
RP   FUNCTION OF TRUNCATED PEPTIDE.
RX   PubMed=11046116;
RA   Hoare S.R.J., Usdin T.B.;
RT   "Tuberoinfundibular peptide (7-39) [TIP(7-39)], a novel, selective, high-
RT   affinity antagonist for the parathyroid hormone-1 receptor with no
RT   detectable agonist activity.";
RL   J. Pharmacol. Exp. Ther. 295:761-770(2000).
CC   -!- FUNCTION: Plays a role as a potent and selective agonist of PTH2R
CC       resulting in adenyl cyclase activation and intracellular calcium levels
CC       elevation. Induces protein kinase C beta activation, recruitment of
CC       beta-arrestin and PTH2R internalization. May inhibit cell proliferation
CC       via its action of PTH2R activation. Neuropeptide which may also have a
CC       role in spermatogenesis. May activate nociceptors and nociceptive
CC       circuits. {ECO:0000269|PubMed:10526330, ECO:0000269|PubMed:11046116}.
CC   -!- SUBUNIT: Ligand of high affinity for the PTH2 receptor (PTH2R).
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- MASS SPECTROMETRY: Mass=4504.45; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:10526330};
CC   -!- MISCELLANEOUS: The N-terminal truncation of tuberoinfundibular peptide
CC       of 39 residues [TIP39(7-39)] reverses PTH2R/PTHR1 binding selectivity,
CC       then is a highly potent and selective antagonist for PTHR1. It displays
CC       a more favorable in vitro pharmacological profile than antagonists
CC       derived from the structure of PTH or PTHLH.
CC   -!- SIMILARITY: Belongs to the parathyroid hormone family. {ECO:0000305}.
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DR   EMBL; AC010619; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   AlphaFoldDB; P0C171; -.
DR   SMR; P0C171; -.
DR   PRIDE; P0C171; -.
DR   InParanoid; P0C171; -.
DR   Proteomes; UP000009136; Unplaced.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0007218; P:neuropeptide signaling pathway; IEA:UniProtKB-KW.
DR   InterPro; IPR029396; TIP39.
DR   PANTHER; PTHR28585; PTHR28585; 1.
DR   Pfam; PF14980; TIP39; 1.
PE   1: Evidence at protein level;
KW   Cleavage on pair of basic residues; Direct protein sequencing;
KW   Neuropeptide; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..30
FT                   /evidence="ECO:0000255"
FT   PROPEP          31..59
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000227668"
FT   PEPTIDE         62..100
FT                   /note="Tuberoinfundibular peptide of 39 residues"
FT                   /id="PRO_0000227669"
SQ   SEQUENCE   100 AA;  11202 MW;  7F0F1A273829E8F6 CRC64;
     METRQVSRSP RVRLLLLLLL LLVVPWGVRT ASGVALPPVG VLSLRPPGRA WADPATPRPR
     RSLALADDAA FRERARLLAA LERRHWLNSY MHKLLVLDAP