ID   TIPL_BPN15              Reviewed;         251 AA.
AC   O64332;
DT   29-APR-2015, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   03-AUG-2022, entry version 55.
DE   RecName: Full=Tail tip protein L {ECO:0000303|PubMed:10860722};
DE   AltName: Full=Gene product 18 {ECO:0000305};
DE            Short=gp18 {ECO:0000305};
GN   Name=gene 18 {ECO:0000312|EMBL:AAC19054.1};
OS   Escherichia phage N15 (Bacteriophage N15).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Siphoviridae; Ravinvirus.
OX   NCBI_TaxID=40631 {ECO:0000312|Proteomes:UP000002132};
OH   NCBI_TaxID=562; Escherichia coli.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND IDENTIFICATION.
RX   PubMed=10860722; DOI=10.1006/jmbi.2000.3731;
RA   Ravin V., Ravin N., Casjens S., Ford M.E., Hatfull G.F., Hendrix R.W.;
RT   "Genomic sequence and analysis of the atypical temperate bacteriophage
RT   N15.";
RL   J. Mol. Biol. 299:53-73(2000).
RN   [2]
RP   DOMAIN, AND COFACTOR.
RX   PubMed=23542343; DOI=10.1016/j.jmb.2013.03.032;
RA   Tam W., Pell L.G., Bona D., Tsai A., Dai X.X., Edwards A.M., Hendrix R.W.,
RA   Maxwell K.L., Davidson A.R.;
RT   "Tail tip proteins related to bacteriophage lambda gpL coordinate an iron-
RT   sulfur cluster.";
RL   J. Mol. Biol. 425:2450-2462(2013).
CC   -!- FUNCTION: Part of the distal tail tip complex which plays a role in DNA
CC       injection during entry, and in tail assembly initiation during exit.
CC       The tail tip complex is assembled successively with three tail central
CC       fiber proteins J, one tail tip protein I, one tail tip protein L and
CC       one tail tip protein K. The tail tip complex interacts with tail
CC       measure protein to initiate tail tube assembly. The formation of the
CC       tail tip complex is completed by the addition of tail tip protein M,
CC       which is followed by tail tube polymerization.
CC       {ECO:0000250|UniProtKB:P03738}.
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC         Evidence={ECO:0000269|PubMed:23542343};
CC       Note=Binds 1 [4Fe-4S] cluster.;
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000250|UniProtKB:P03738}. Host
CC       cytoplasm {ECO:0000250|UniProtKB:P03738}.
CC   -!- DOMAIN: The C-terminus coordinates an iron-sulfur cluster.
CC       {ECO:0000269|PubMed:23542343}.
CC   -!- SIMILARITY: Belongs to the lambda-like tail tip protein L family.
CC       {ECO:0000305}.
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DR   EMBL; AF064539; AAC19054.1; -; Genomic_DNA.
DR   PIR; T13104; T13104.
DR   RefSeq; NP_046913.1; NC_001901.1.
DR   GeneID; 1261657; -.
DR   KEGG; vg:1261657; -.
DR   Proteomes; UP000002132; Genome.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0098015; C:virus tail; IEA:UniProtKB-KW.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IDA:UniProtKB.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0099001; P:viral genome ejection through host cell envelope, long flexible tail mechanism; IEA:UniProtKB-KW.
DR   GO; GO:0098003; P:viral tail assembly; IEA:UniProtKB-KW.
DR   InterPro; IPR006487; Phage_lambda_L.
DR   Pfam; PF05100; Phage_tail_L; 1.
DR   TIGRFAMs; TIGR01600; phage_tail_L; 1.
PE   3: Inferred from homology;
KW   4Fe-4S; Host cytoplasm; Iron; Iron-sulfur; Late protein; Metal-binding;
KW   Reference proteome; Viral genome ejection through host cell envelope;
KW   Viral long flexible tail ejection system;
KW   Viral penetration into host cytoplasm; Viral release from host cell;
KW   Viral tail assembly; Viral tail protein; Virion;
KW   Virus entry into host cell.
FT   CHAIN           1..251
FT                   /note="Tail tip protein L"
FT                   /id="PRO_0000432902"
FT   BINDING         184
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000269|PubMed:23542343"
FT   BINDING         198
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000269|PubMed:23542343"
FT   BINDING         221
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000269|PubMed:23542343"
FT   BINDING         228
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000269|PubMed:23542343"
SQ   SEQUENCE   251 AA;  27847 MW;  2FBC2B408203F239 CRC64;
     MSLNADYQKL EPGNEVRLFS VDGTAFGMSD VLRFHAHNIA HTPEEIEAAG GDENKLPAKS
     IWWQGEEYKA WPCQVEGIEA TTDGTSPQPK LTVANLDSSI SALCLAYDDL LQAKVTIHDT
     LAKYLDARNF PQGNPTADPS QEKLKVFYID ARNTETDEVV EFILSSPMDL QGRMIPTRQL
     HSLCSWCIRN KYRTGDGCDY AGTRYFDKHN NPVDDPSLDE CNGTLTACKL RHGDSNELPF
     GGFPGTSLIR S