AC4_BCTVC
ID AC4_BCTVC Reviewed; 85 AA.
AC Q91J24; D4PH95;
DT 15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 15-MAY-2007, sequence version 2.
DT 03-AUG-2022, entry version 54.
DE RecName: Full=Protein C4;
DE AltName: Full=Protein L4;
GN ORFNames=C4, L4;
OS Beet curly top virus (strain California/Logan) (BCTV).
OC Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes;
OC Geplafuvirales; Geminiviridae; Curtovirus.
OX NCBI_TaxID=268960;
OH NCBI_TaxID=161934; Beta vulgaris (Sugar beet).
OH NCBI_TaxID=4071; Capsicum (peppers).
OH NCBI_TaxID=3650; Cucurbitaceae.
OH NCBI_TaxID=4005; Linum.
OH NCBI_TaxID=3885; Phaseolus vulgaris (Kidney bean) (French bean).
OH NCBI_TaxID=4081; Solanum lycopersicum (Tomato) (Lycopersicon esculentum).
OH NCBI_TaxID=4113; Solanum tuberosum (Potato).
OH NCBI_TaxID=3562; Spinacia oleracea (Spinach).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Infectious clone pBCT028;
RX PubMed=16453696; DOI=10.1002/j.1460-2075.1986.tb04424.x;
RA Stanley J., Markham P.G., Callis R.J., Pinner M.S.;
RT "The nucleotide sequence of an infectious clone of the geminivirus beet
RT curly top virus.";
RL EMBO J. 5:1761-1767(1986).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Bisaro D.M., Hormuzdi S.G.;
RL Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP FUNCTION.
RX PubMed=1529551; DOI=10.1016/0042-6822(92)91243-n;
RA Stanley J., Latham J.R.;
RT "A symptom variant of beet curly top geminivirus produced by mutation of
RT open reading frame C4.";
RL Virology 190:506-509(1992).
RN [4]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX DOI=10.1046/j.1365-313X.1997.11061273.x;
RA Latham J.R., Saunders K., Pinner M.S., Stanley J.;
RT "Induction of plant cell division by beet curly top virus gene C4.";
RL Plant J. 11:1273-1283(1997).
RN [5]
RP SUBCELLULAR LOCATION, INTERACTION WITH ARABIDOPSIS THALIANA RCH2;
RP ASK7/ASK-ETA AND ASK6/ASK-ZETA, MUTAGENESIS OF GLY-2; PRO-44; ALA-45;
RP SER-49 AND PRO-50, MYRISTOYLATION AT GLY-2, AND PHOSPHORYLATION BY
RP ARABIDOPSIS THALIANA ASK7/ASK-ETA.
RX PubMed=17280695; DOI=10.1016/j.virol.2006.12.034;
RA Piroux N., Saunders K., Page A., Stanley J.;
RT "Geminivirus pathogenicity protein C4 interacts with Arabidopsis thaliana
RT shaggy-related protein kinase AtSKeta, a component of the brassinosteroid
RT signalling pathway.";
RL Virology 362:428-440(2007).
CC -!- FUNCTION: Major determinant of pathogenesis that affects the
CC hyperplastic response of the host to viral infection. Mediates the
CC induction of cell division in permissive cells, mainly in phloem. May
CC act as a suppressor of RNA-mediated gene silencing, also known as post-
CC transcriptional gene silencing (PTGS), a mechanism of plant viral
CC defense that limits the accumulation of viral RNAs.
CC {ECO:0000269|PubMed:1529551, ECO:0000269|Ref.4}.
CC -!- SUBUNIT: Interacts with Arabidopsis thaliana RCH2, ASK7/ASK-eta and
CC ASK6/ASK-zeta. {ECO:0000269|PubMed:17280695}.
CC -!- SUBCELLULAR LOCATION: Host cell membrane {ECO:0000269|PubMed:17280695};
CC Lipid-anchor {ECO:0000269|PubMed:17280695}. Note=Localizes to the cell
CC periphery.
CC -!- TISSUE SPECIFICITY: Expressed in vascular tissues, and especially in
CC phloem cells. {ECO:0000269|Ref.4}.
CC -!- PTM: Phosphorylated by Arabidopsis thaliana ASK7/ASK-eta mainly on
CC threonine and serine residues. {ECO:0000269|PubMed:17280695}.
CC -!- SIMILARITY: Belongs to the geminiviridae protein AC4/C4 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAK59259.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; M24597; ADD82466.1; -; Genomic_DNA.
DR EMBL; AF379637; AAK59259.1; ALT_INIT; Genomic_DNA.
DR SMR; Q91J24; -.
DR iPTMnet; Q91J24; -.
DR SwissPalm; Q91J24; -.
DR Proteomes; UP000006542; Genome.
DR GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR InterPro; IPR002488; Gemini_C4.
DR Pfam; PF01492; Gemini_C4; 1.
PE 1: Evidence at protein level;
KW Host cell membrane; Host membrane; Host-virus interaction; Lipoprotein;
KW Membrane; Myristate; Phosphoprotein; Reference proteome;
KW Suppressor of RNA silencing.
FT INIT_MET 1
FT /note="Removed; by host"
FT /evidence="ECO:0000269|PubMed:17280695"
FT CHAIN 2..85
FT /note="Protein C4"
FT /id="PRO_0000287219"
FT REGION 42..65
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT LIPID 2
FT /note="N-myristoyl glycine; by host"
FT /evidence="ECO:0000269|PubMed:17280695"
FT VARIANT 37
FT /note="R -> Q (in strain: Infectious clone pBCT028)"
FT VARIANT 62
FT /note="G -> E (in strain: Infectious clone pBCT028)"
FT MUTAGEN 2
FT /note="G->A: Reduced pathogenicity on N. benthamiana, and
FT loss of anchoring to the plasma membrane."
FT /evidence="ECO:0000269|PubMed:17280695"
FT MUTAGEN 44
FT /note="P->R: Reduced pathogenicity on N. benthamiana."
FT /evidence="ECO:0000269|PubMed:17280695"
FT MUTAGEN 45
FT /note="A->L: Reduced pathogenicity on N. benthamiana."
FT /evidence="ECO:0000269|PubMed:17280695"
FT MUTAGEN 49
FT /note="S->R: Reduced pathogenicity on N. benthamiana."
FT /evidence="ECO:0000269|PubMed:17280695"
FT MUTAGEN 50
FT /note="P->R: Reduced pathogenicity on N. benthamiana."
FT /evidence="ECO:0000269|PubMed:17280695"
SQ SEQUENCE 85 AA; 9688 MW; 4AC32182F0EEB786 CRC64;
MGNLISTSCF NSKEKFRSQI SDYSTWYPQP GQHISIRTFR ELNPAPTSSP TSTRTETQLN
GGNSRSTVEV LEEVNRQLTT HMPRR
