ID   TIPL_LAMBD              Reviewed;         232 AA.
AC   P03738;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   21-JUL-1986, sequence version 1.
DT   03-AUG-2022, entry version 71.
DE   RecName: Full=Tail tip protein L;
GN   Name=L; OrderedLocusNames=lambdap18;
OS   Escherichia phage lambda (Bacteriophage lambda).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Siphoviridae; Lambdavirus.
OX   NCBI_TaxID=10710;
OH   NCBI_TaxID=562; Escherichia coli.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=6221115; DOI=10.1016/0022-2836(82)90546-0;
RA   Sanger F., Coulson A.R., Hong G.F., Hill D.F., Petersen G.B.;
RT   "Nucleotide sequence of bacteriophage lambda DNA.";
RL   J. Mol. Biol. 162:729-773(1982).
RN   [2]
RP   FUNCTION.
RX   PubMed=1003470; DOI=10.1016/s0022-2836(76)80007-1;
RA   Katsura I.;
RT   "Morphogenesis of bacteriophage lambda tail. Polymorphism in the assembly
RT   of the major tail protein.";
RL   J. Mol. Biol. 107:307-326(1976).
RN   [3]
RP   FUNCTION, AND MUTAGENESIS OF CYS-173; CYS-182; CYS-205 AND CYS-212.
RX   PubMed=23542343; DOI=10.1016/j.jmb.2013.03.032;
RA   Tam W., Pell L.G., Bona D., Tsai A., Dai X.X., Edwards A.M., Hendrix R.W.,
RA   Maxwell K.L., Davidson A.R.;
RT   "Tail tip proteins related to bacteriophage lambda gpL coordinate an iron-
RT   sulfur cluster.";
RL   J. Mol. Biol. 425:2450-2462(2013).
CC   -!- FUNCTION: Part of the distal tail tip complex which plays a role in DNA
CC       ejection during entry, and in tail assembly initiation during exit. The
CC       tail tip complex is assembled successively with three tail tip proteins
CC       J, one tail tip protein I, one tail tip protein L and one tail tip
CC       protein K. The tail tip complex interacts with tail measure protein to
CC       initiate tail tube assembly. The formation of the tail tip complex is
CC       completed by the addition of tail tip protein M, which is followed by
CC       tail tube polymerization. {ECO:0000269|PubMed:1003470,
CC       ECO:0000269|PubMed:23542343}.
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC         Evidence={ECO:0000250|UniProtKB:O64332};
CC       Note=Binds 1 [4Fe-4S] cluster.;
CC   -!- SUBCELLULAR LOCATION: Virion. Host cytoplasm.
CC   -!- DOMAIN: The C-terminus coordinates an iron-sulfur cluster.
CC       {ECO:0000250|UniProtKB:O64332}.
CC   -!- SIMILARITY: Belongs to the lambda-like tail tip protein L family.
CC       {ECO:0000305}.
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DR   EMBL; J02459; AAA96550.1; -; Genomic_DNA.
DR   PIR; A43009; TLBPLL.
DR   RefSeq; NP_040597.1; NC_001416.1.
DR   IntAct; P03738; 1.
DR   GeneID; 2703513; -.
DR   KEGG; vg:2703513; -.
DR   Proteomes; UP000001711; Genome.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0098015; C:virus tail; IEA:UniProtKB-KW.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0099001; P:viral genome ejection through host cell envelope, long flexible tail mechanism; IEA:UniProtKB-KW.
DR   GO; GO:0098003; P:viral tail assembly; IMP:UniProtKB.
DR   InterPro; IPR006487; Phage_lambda_L.
DR   Pfam; PF05100; Phage_tail_L; 1.
DR   TIGRFAMs; TIGR01600; phage_tail_L; 1.
PE   1: Evidence at protein level;
KW   4Fe-4S; Host cytoplasm; Iron; Iron-sulfur; Late protein; Metal-binding;
KW   Reference proteome; Viral genome ejection through host cell envelope;
KW   Viral long flexible tail ejection system;
KW   Viral penetration into host cytoplasm; Viral release from host cell;
KW   Viral tail assembly; Viral tail protein; Virion;
KW   Virus entry into host cell.
FT   CHAIN           1..232
FT                   /note="Tail tip protein L"
FT                   /id="PRO_0000077667"
FT   BINDING         173
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250|UniProtKB:O64332"
FT   BINDING         182
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250|UniProtKB:O64332"
FT   BINDING         205
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250|UniProtKB:O64332"
FT   BINDING         212
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250|UniProtKB:O64332"
FT   MUTAGEN         173
FT                   /note="C->S: Complete loss of tail assembly."
FT                   /evidence="ECO:0000269|PubMed:23542343"
FT   MUTAGEN         182
FT                   /note="C->S: Complete loss of tail assembly."
FT                   /evidence="ECO:0000269|PubMed:23542343"
FT   MUTAGEN         205
FT                   /note="C->S: Complete loss of tail assembly."
FT                   /evidence="ECO:0000269|PubMed:23542343"
FT   MUTAGEN         212
FT                   /note="C->S: 96% loss of tail assembly."
FT                   /evidence="ECO:0000269|PubMed:23542343"
SQ   SEQUENCE   232 AA;  25710 MW;  44397934E14ADCBE CRC64;
     MQDIRQETLN ECTRAEQSAS VVLWEIDLTE VGGERYFFCN EQNEKGEPVT WQGRQYQPYP
     IQGSGFELNG KGTSTRPTLT VSNLYGMVTG MAEDMQSLVG GTVVRRKVYA RFLDAVNFVN
     GNSYADPEQE VISRWRIEQC SELSAVSASF VLSTPTETDG AVFPGRIMLA NTCTWTYRGD
     ECGYSGPAVA DEYDQPTSDI TKDKCSKCLS GCKFRNNVGN FGGFLSINKL SQ