TIPT_DROME
ID TIPT_DROME Reviewed; 1024 AA.
AC Q9U3V5; Q95SG6; Q9V9P4;
DT 29-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT 24-OCT-2003, sequence version 2.
DT 03-AUG-2022, entry version 149.
DE RecName: Full=Protein tiptop;
GN Name=tio; ORFNames=CG12630;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=15936749; DOI=10.1016/j.ydbio.2005.05.005;
RA Laugier E., Yang Z., Fasano L., Kerridge S., Vola C.;
RT "A critical role of teashirt for patterning the ventral epidermis is masked
RT by ectopic expression of tiptop, a paralog of teashirt in Drosophila.";
RL Dev. Biol. 283:446-458(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-18.
RC STRAIN=Berkeley; TISSUE=Head;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC -!- FUNCTION: Tiptop (tio) and teashirt (tsh) have, on the whole, common
CC activities. Tio and tsh repress each other's expression and tsh has a
CC crucial role for trunk patterning that is in part masked by ectopic
CC expression of tiptop. Both genes share a common activity required for
CC the activation of Ser and svb and the maintenance of en and wg.
CC {ECO:0000269|PubMed:15936749}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expression in the Malpighian tubules (MTs) and
CC stomatogastric nervous system starts at embryonic stage 10. At stage
CC 11, expression in the head domain is initiated in the clypeolabrum in
CC two bilaterally symmetric clusters of cells. At stage 12, expression
CC appears in the central nervous system (CNS) of the trunk and the
CC epidermis. The staining in the hindgut is maintained throughout
CC embryogenesis. At stage 13, expression is present in elongating MTs.
CC The anterior staining is detected in cells that invaginate into the
CC stomodeum and by stage 15 onwards, in cells close to the pharynx. Also
CC expressed in cells of the brain, the second constriction of the gut,
CC the trunk epidermis, the anterior segments of the CNS (the three
CC thoracic and the first two abdominal segments) and in the MTs. From
CC stage 12 onwards, tsh and tio are colocalized in some cells.
CC {ECO:0000269|PubMed:15936749}.
CC -!- MISCELLANEOUS: The tsh tio gene pair seems to have arisen from a recent
CC duplication event: tsh has the dominant role compared to tio.
CC -!- SIMILARITY: Belongs to the teashirt C2H2-type zinc-finger protein
CC family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF23183.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=AAL28355.1; Type=Erroneous translation; Note=Wrong choice of frame.; Evidence={ECO:0000305};
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DR EMBL; AF219383; AAF23183.1; ALT_INIT; mRNA.
DR EMBL; AE014134; AAF57242.3; -; Genomic_DNA.
DR EMBL; AY060807; AAL28355.1; ALT_SEQ; mRNA.
DR RefSeq; NP_001260679.1; NM_001273750.1.
DR RefSeq; NP_524733.2; NM_079994.4.
DR AlphaFoldDB; Q9U3V5; -.
DR BioGRID; 68936; 11.
DR IntAct; Q9U3V5; 9.
DR STRING; 7227.FBpp0088404; -.
DR PaxDb; Q9U3V5; -.
DR EnsemblMetazoa; FBtr0089377; FBpp0088404; FBgn0028979.
DR EnsemblMetazoa; FBtr0335391; FBpp0307374; FBgn0028979.
DR GeneID; 44272; -.
DR KEGG; dme:Dmel_CG12630; -.
DR UCSC; CG12630-RA; d. melanogaster.
DR CTD; 44272; -.
DR FlyBase; FBgn0028979; tio.
DR VEuPathDB; VectorBase:FBgn0028979; -.
DR eggNOG; ENOG502QV71; Eukaryota.
DR GeneTree; ENSGT00950000183051; -.
DR HOGENOM; CLU_007777_0_0_1; -.
DR InParanoid; Q9U3V5; -.
DR OMA; GHCFNNN; -.
DR OrthoDB; 967773at2759; -.
DR PhylomeDB; Q9U3V5; -.
DR BioGRID-ORCS; 44272; 0 hits in 3 CRISPR screens.
DR ChiTaRS; neb; fly.
DR GenomeRNAi; 44272; -.
DR PRO; PR:Q9U3V5; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0028979; Expressed in testis and 11 other tissues.
DR ExpressionAtlas; Q9U3V5; baseline and differential.
DR Genevisible; Q9U3V5; DM.
DR GO; GO:0005634; C:nucleus; IDA:FlyBase.
DR GO; GO:0003677; F:DNA binding; IBA:GO_Central.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0048749; P:compound eye development; IMP:FlyBase.
DR GO; GO:0048730; P:epidermis morphogenesis; IMP:FlyBase.
DR GO; GO:0061330; P:Malpighian tubule stellate cell differentiation; IGI:FlyBase.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IMP:FlyBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0007380; P:specification of segmental identity, head; IMP:FlyBase.
DR InterPro; IPR027008; Teashirt_fam.
DR InterPro; IPR026807; Tio/Tsh.
DR InterPro; IPR041661; ZN622/Rei1/Reh1_Znf-C2H2.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR PANTHER; PTHR12487; PTHR12487; 1.
DR PANTHER; PTHR12487:SF7; PTHR12487:SF7; 1.
DR Pfam; PF00096; zf-C2H2; 1.
DR Pfam; PF12756; zf-C2H2_2; 2.
DR SMART; SM00355; ZnF_C2H2; 4.
DR SUPFAM; SSF57667; SSF57667; 1.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 4.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 3.
PE 2: Evidence at transcript level;
KW Activator; Developmental protein; DNA-binding; Metal-binding; Nucleus;
KW Reference proteome; Repeat; Repressor; Transcription;
KW Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..1024
FT /note="Protein tiptop"
FT /id="PRO_0000047068"
FT ZN_FING 317..341
FT /note="C2H2-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 426..450
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 499..523
FT /note="C2H2-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 926..949
FT /note="C2H2-type 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT REGION 20..40
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 138..215
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 350..392
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 466..489
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 519..576
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 712..759
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 786..818
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 868..891
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 954..1004
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 20..35
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 144..160
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 161..175
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 362..392
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 472..489
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 712..741
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 742..759
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 798..818
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 969..988
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1024 AA; 108364 MW; 93888A08467C4F5C CRC64;
MMLHEAVMLE IYRQALSASE LTSPRCQSRD SNTSAGAGAG MADVRCPSNE SHCSANDRLT
PAATPTLTPT EATISPNSVG LPLTATLPPA AAVALLPPQS AAMAAYLAAA QQNHLLLTNP
LAAAASLVQH ATQQAVVEGE VESPALDFSR KRPKSHGDDD QEEDQEQDQE QEQEQEPDHD
VQCDNGPLDL SVSTGKRQES VSPPARKIPR SISADYKSPL PPGSWMPPIN PYLAAVAAKT
GGLGYSKLAP SEASKALEKM TEMSRLETSP TAARSLGATS SVGAGVPAGA SSNSGGRHSA
WQSHWLNKGA DTAKDVFKCV WCKQSFSTLA NLTAHMKETQ HCGVQIPSPL PTGGVGTPSA
PPPTRLATSA SNSACSSSSS STSSSSNSSK SELNMLIKET MPLPRKLVRG QDVWLGKGAE
QTRQILKCMW CGQSFRSLAE MTSHMQETQH YTNIISQEQI ISWKSGDERE RPTNTGVPST
STAAPSSPSC TAPSVSAVLT CKVCDQAFGS LKELSTHMAQ KSHYKESPAP SASPPAAGTG
NPKRGRQNRN EKRKKSLPVR KLLELERSGS NSSLDSALKP LRDFAAATKI TCEKCGSKIE
TALFVEHIRK CLGESIPIPP RRSNAGVDRL PSPSLGLGAE KPPSVLNALE QLIEKSFESR
TSRTMTHGGY SEAGTPLGAS ILKRLGIEDS SDYTKPLMDA QAMHLLRSSF ASRDRSASES
SSASRVESSY TPDRQQATPH KSPDTPAPPP PPPPTIKAEP LEAEPLVGCD REGCSPRQQI
QVKKEFSMEA CRESPRSVSK SPAPQTERSP PDNGSLLALN SMFDQLSGVE NSGNNNSGHC
FNNNNSCSSV SAQKPKAHPL AALQKLCETT DPPSTGLRSA SSAGSSTASA TLPSANGNDL
VAFSWACNEA VLSASNGGSA GDSSIIKCSY CDTPFASKGA YRHHLSKVHF VKDAGEDSPR
LKSPAVQSPR SMPLASPRRS ASRSPATGSQ QPPPSPTISP YDESPQSKFL KYTELAKQLS
SKNA
