ID   TIP_ANTMA               Reviewed;         250 AA.
AC   P33560;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   25-MAY-2022, entry version 94.
DE   RecName: Full=Probable aquaporin TIP-type;
DE   AltName: Full=Dark intrinsic protein;
DE   AltName: Full=Tonoplast intrinsic protein DiP;
GN   Name=DIP;
OS   Antirrhinum majus (Garden snapdragon).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Lamiales; Plantaginaceae; Antirrhineae; Antirrhinum.
OX   NCBI_TaxID=4151;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. JI:522; TISSUE=Seedling;
RX   PubMed=7504555; DOI=10.1046/j.1365-313x.1993.04040717.x;
RA   Culianez-Macia F.A., Martin C.R.;
RT   "DIP: a member of the MIP family of membrane proteins that is expressed in
RT   mature seeds and dark-grown seedlings of Antirrhinum majus.";
RL   Plant J. 4:717-725(1993).
CC   -!- FUNCTION: Channel protein in tonoplast. These proteins may allow the
CC       diffusion of amino acids and/or peptides from the vacuolar compartment
CC       to the cytoplasm (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Vacuole membrane {ECO:0000305}; Multi-pass
CC       membrane protein {ECO:0000305}. Note=Tonoplast.
CC   -!- TISSUE SPECIFICITY: Expressed in mature seeds and dark-grown seedlings.
CC   -!- DOMAIN: Aquaporins contain two tandem repeats each containing three
CC       membrane-spanning domains and a pore-forming loop with the signature
CC       motif Asn-Pro-Ala (NPA).
CC   -!- SIMILARITY: Belongs to the MIP/aquaporin (TC 1.A.8) family. TIP (TC
CC       1.A.8.10) subfamily. {ECO:0000305}.
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DR   EMBL; X70417; CAA49854.1; -; mRNA.
DR   PIR; S48116; S48116.
DR   PIR; S51781; S51781.
DR   AlphaFoldDB; P33560; -.
DR   SMR; P33560; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005774; C:vacuolar membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015267; F:channel activity; IEA:InterPro.
DR   CDD; cd00333; MIP; 1.
DR   Gene3D; 1.20.1080.10; -; 1.
DR   InterPro; IPR023271; Aquaporin-like.
DR   InterPro; IPR034294; Aquaporin_transptr.
DR   InterPro; IPR000425; MIP.
DR   InterPro; IPR022357; MIP_CS.
DR   PANTHER; PTHR45665; PTHR45665; 1.
DR   Pfam; PF00230; MIP; 1.
DR   PRINTS; PR00783; MINTRINSICP.
DR   SUPFAM; SSF81338; SSF81338; 1.
DR   TIGRFAMs; TIGR00861; MIP; 1.
DR   PROSITE; PS00221; MIP; 1.
PE   2: Evidence at transcript level;
KW   Membrane; Repeat; Transmembrane; Transmembrane helix; Transport; Vacuole.
FT   CHAIN           1..250
FT                   /note="Probable aquaporin TIP-type"
FT                   /id="PRO_0000064018"
FT   TRANSMEM        20..42
FT                   /note="Helical; Name=1"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        55..77
FT                   /note="Helical; Name=2"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        97..119
FT                   /note="Helical; Name=3"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        140..162
FT                   /note="Helical; Name=4"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        172..194
FT                   /note="Helical; Name=5"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        215..237
FT                   /note="Helical; Name=6"
FT                   /evidence="ECO:0000255"
FT   MOTIF           83..85
FT                   /note="NPA 1"
FT   MOTIF           197..199
FT                   /note="NPA 2"
SQ   SEQUENCE   250 AA;  25210 MW;  DE595778E12D7808 CRC64;
     MVKIAFGSIG DSFSVASIKA YVAEFIATLL FVFAGVGSAI AYNKLTSDAA LDPAGLVAVA
     VAHAFALFVG VSMAANVSGG HLNPAVTLGL AVGGNITILT GLFYWIAQCL GSTVACLLLK
     FVTNGLSVPT HGVAAGMDAI QGVVMEIIIT FALVYTVYAT AADPKKGSLG VIAPIAIGFI
     VGANILAAGP FSGGSMNPAR SFGPAVASGD FSQNWIYWAG PLIGGALAGF IYGDVFITAH
     APLPTSEDYA