TIP_HUMAN
ID TIP_HUMAN Reviewed; 612 AA.
AC Q8TB96; Q96SR4; Q9BRE2; Q9H2V9;
DT 15-MAR-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=T-cell immunomodulatory protein;
DE Short=Protein TIP;
DE AltName: Full=Integrin-alpha FG-GAP repeat-containing protein 1;
DE AltName: Full=Linkin {ECO:0000303|PubMed:25437307};
DE Flags: Precursor;
GN Name=ITFG1 {ECO:0000312|HGNC:HGNC:30697};
GN Synonyms=LNKN-1 {ECO:0000303|PubMed:25437307},
GN TIP {ECO:0000312|HGNC:HGNC:30697}; ORFNames=CDA08;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND SUBCELLULAR LOCATION.
RX PubMed=12598909; DOI=10.1038/nbt797;
RA Fiscella M., Perry J.W., Teng B., Bloom M., Zhang C., Leung K., Pukac L.,
RA Florence K., Concepcion A., Liu B., Meng Y., Chen C., Elgin E.C.,
RA Kanakaraj P., Kaufmann T.E., Porter J., Cibotti R., Mei Y., Zhou J.,
RA Chen G., Roschke V., Komatsoulis G., Mansfield B., Ruben S., Sanyal I.,
RA Migone T.-S.;
RT "TIP, a T-cell factor identified using high-throughput screening increases
RT survival in a graft-versus-host disease model.";
RL Nat. Biotechnol. 21:302-307(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Pheochromocytoma;
RA Li Y., Huang Q., Peng Y., Song H., Yu Y., Xu S., Ren S., Chen Z., Han Z.;
RT "A novel gene expressed in human pheochromocytoma.";
RL Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Muscle, and Skin;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-188.
RC TISSUE=Liver;
RX PubMed=19159218; DOI=10.1021/pr8008012;
RA Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.;
RT "Glycoproteomics analysis of human liver tissue by combination of multiple
RT enzyme digestion and hydrazide chemistry.";
RL J. Proteome Res. 8:651-661(2009).
RN [6]
RP INTERACTION WITH RUVBL1; RUVBL2 AND ALPHA-TUBULIN.
RX PubMed=25437307; DOI=10.7554/elife.04449;
RA Kato M., Chou T.F., Yu C.Z., DeModena J., Sternberg P.W.;
RT "LINKIN, a new transmembrane protein necessary for cell adhesion.";
RL Elife 3:E04449-E04449(2014).
CC -!- FUNCTION: Modulator of T-cell function. Has a protective effect in
CC graft versus host disease model (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Interacts with RUVBL1, RUVBL2 and alpha-tubulin.
CC {ECO:0000269|PubMed:25437307}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}. Membrane {ECO:0000305};
CC Single-pass type I membrane protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Ubiquitously expressed.
CC -!- SIMILARITY: Belongs to the TIP family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG41782.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AF503339; AAN31655.1; -; mRNA.
DR EMBL; AF212247; AAG41782.1; ALT_FRAME; mRNA.
DR EMBL; AK027596; BAB55220.1; -; mRNA.
DR EMBL; BC006321; AAH06321.2; -; mRNA.
DR EMBL; BC024162; AAH24162.1; -; mRNA.
DR CCDS; CCDS10728.1; -.
DR RefSeq; NP_001291931.1; NM_001305002.1.
DR RefSeq; NP_110417.2; NM_030790.4.
DR AlphaFoldDB; Q8TB96; -.
DR BioGRID; 123507; 582.
DR IntAct; Q8TB96; 15.
DR MINT; Q8TB96; -.
DR STRING; 9606.ENSP00000319918; -.
DR GlyConnect; 1789; 1 N-Linked glycan (1 site).
DR GlyGen; Q8TB96; 12 sites, 1 N-linked glycan (1 site).
DR iPTMnet; Q8TB96; -.
DR PhosphoSitePlus; Q8TB96; -.
DR BioMuta; ITFG1; -.
DR DMDM; 45477238; -.
DR EPD; Q8TB96; -.
DR jPOST; Q8TB96; -.
DR MassIVE; Q8TB96; -.
DR MaxQB; Q8TB96; -.
DR PaxDb; Q8TB96; -.
DR PeptideAtlas; Q8TB96; -.
DR PRIDE; Q8TB96; -.
DR ProteomicsDB; 73977; -.
DR Antibodypedia; 14372; 171 antibodies from 23 providers.
DR DNASU; 81533; -.
DR Ensembl; ENST00000320640.11; ENSP00000319918.6; ENSG00000129636.13.
DR GeneID; 81533; -.
DR KEGG; hsa:81533; -.
DR MANE-Select; ENST00000320640.11; ENSP00000319918.6; NM_030790.5; NP_110417.2.
DR UCSC; uc002eet.4; human.
DR CTD; 81533; -.
DR DisGeNET; 81533; -.
DR GeneCards; ITFG1; -.
DR HGNC; HGNC:30697; ITFG1.
DR HPA; ENSG00000129636; Low tissue specificity.
DR MIM; 611803; gene.
DR neXtProt; NX_Q8TB96; -.
DR OpenTargets; ENSG00000129636; -.
DR PharmGKB; PA143485506; -.
DR VEuPathDB; HostDB:ENSG00000129636; -.
DR eggNOG; KOG4550; Eukaryota.
DR GeneTree; ENSGT00390000013367; -.
DR HOGENOM; CLU_020272_2_0_1; -.
DR InParanoid; Q8TB96; -.
DR OMA; PGDWIPW; -.
DR OrthoDB; 790976at2759; -.
DR PhylomeDB; Q8TB96; -.
DR TreeFam; TF105620; -.
DR PathwayCommons; Q8TB96; -.
DR SignaLink; Q8TB96; -.
DR BioGRID-ORCS; 81533; 16 hits in 1082 CRISPR screens.
DR ChiTaRS; ITFG1; human.
DR GenomeRNAi; 81533; -.
DR Pharos; Q8TB96; Tbio.
DR PRO; PR:Q8TB96; -.
DR Proteomes; UP000005640; Chromosome 16.
DR RNAct; Q8TB96; protein.
DR Bgee; ENSG00000129636; Expressed in adrenal tissue and 195 other tissues.
DR ExpressionAtlas; Q8TB96; baseline and differential.
DR Genevisible; Q8TB96; HS.
DR GO; GO:0070062; C:extracellular exosome; HDA:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR Gene3D; 2.130.10.130; -; 1.
DR InterPro; IPR013517; FG-GAP.
DR InterPro; IPR028994; Integrin_alpha_N.
DR InterPro; IPR024881; Tip.
DR PANTHER; PTHR13412; PTHR13412; 1.
DR Pfam; PF13517; FG-GAP_3; 1.
DR SUPFAM; SSF69318; SSF69318; 1.
PE 1: Evidence at protein level;
KW Glycoprotein; Membrane; Reference proteome; Secreted; Signal;
KW Transmembrane; Transmembrane helix.
FT SIGNAL 1..33
FT /evidence="ECO:0000255"
FT CHAIN 34..612
FT /note="T-cell immunomodulatory protein"
FT /id="PRO_0000034354"
FT TRANSMEM 567..587
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REPEAT 258..293
FT /note="FG-GAP; atypical"
FT CARBOHYD 36
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 95
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 139
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 146
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 151
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 176
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 188
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:19159218"
FT CARBOHYD 226
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 243
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 353
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 371
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 482
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 612 AA; 68108 MW; CF01ECDCEBB3292A CRC64;
MAAAGRLPSS WALFSPLLAG LALLGVGPVP ARALHNVTAE LFGAEAWGTL AAFGDLNSDK
QTDLFVLRER NDLIVFLADQ NAPYFKPKVK VSFKNHSALI TSVVPGDYDG DSQMDVLLTY
LPKNYAKSEL GAVIFWGQNQ TLDPNNMTIL NRTFQDEPLI MDFNGDLIPD IFGITNESNQ
PQILLGGNLS WHPALTTTSK MRIPHSHAFI DLTEDFTADL FLTTLNATTS TFQFEIWENL
DGNFSVSTIL EKPQNMMVVG QSAFADFDGD GHMDHLLPGC EDKNCQKSTI YLVRSGMKQW
VPVLQDFSNK GTLWGFVPFV DEQQPTEIPI PITLHIGDYN MDGYPDALVI LKNTSGSNQQ
AFLLENVPCN NASCEEARRM FKVYWELTDL NQIKDAMVAT FFDIYEDGIL DIVVLSKGYT
KNDFAIHTLK NNFEADAYFV KVIVLSGLCS NDCPRKITPF GVNQPGPYIM YTTVDANGYL
KNGSAGQLSQ SAHLALQLPY NVLGLGRSAN FLDHLYVGIP RPSGEKSIRK QEWTAIIPNS
QLIVIPYPHN VPRSWSAKLY LTPSNIVLLT AIALIGVCVF ILAIIGILHW QEKKADDREK
RQEAHRFHFD AM
