TIR1_YEAST
ID TIR1_YEAST Reviewed; 254 AA.
AC P10863; D3DLQ8;
DT 01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1989, sequence version 1.
DT 03-AUG-2022, entry version 172.
DE RecName: Full=Cold shock-induced protein TIR1;
DE AltName: Full=Serine-rich protein 1;
DE AltName: Full=TIP1-related protein 1;
DE Flags: Precursor;
GN Name=TIR1; Synonyms=SRP1; OrderedLocusNames=YER011W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3139887; DOI=10.1016/0022-2836(88)90278-1;
RA Marguet D., Guo X.J., Lauquin G.J.-M.;
RT "Yeast gene SRP1 (serine-rich protein). Intragenic repeat structure and
RT identification of a family of SRP1-related DNA sequences.";
RL J. Mol. Biol. 202:455-470(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169868;
RA Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E.,
RA Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E.,
RA Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S.,
RA Hyman R.W., Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D.,
RA Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y.,
RA Botstein D., Davis R.W.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome V.";
RL Nature 387:78-81(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP PRESENCE OF A PIR1/2/3 REPEAT.
RA Bairoch A.;
RL Unpublished observations (OCT-1993).
RN [5]
RP INDUCTION, AND SUBCELLULAR LOCATION.
RX PubMed=7746155; DOI=10.1111/j.1365-2958.1995.tb02248.x;
RA Kowalski L.R.Z., Kondo K., Inouye M.;
RT "Cold-shock induction of a family of TIP1-related proteins associated with
RT the membrane in Saccharomyces cerevisiae.";
RL Mol. Microbiol. 15:341-353(1995).
RN [6]
RP FUNCTION, AND INDUCTION.
RX PubMed=11292809; DOI=10.1128/jb.183.9.2881-2887.2001;
RA Abramova N.E., Sertil O., Mehta S., Lowry C.V.;
RT "Reciprocal regulation of anaerobic and aerobic cell wall mannoprotein gene
RT expression in Saccharomyces cerevisiae.";
RL J. Bacteriol. 183:2881-2887(2001).
RN [7]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [8]
RP SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY, AND GPI-ANCHOR.
RX PubMed=15781460; DOI=10.1074/jbc.m500334200;
RA Yin Q.Y., de Groot P.W.J., Dekker H.L., de Jong L., Klis F.M.,
RA de Koster C.G.;
RT "Comprehensive proteomic analysis of Saccharomyces cerevisiae cell walls:
RT identification of proteins covalently attached via
RT glycosylphosphatidylinositol remnants or mild alkali-sensitive linkages.";
RL J. Biol. Chem. 280:20894-20901(2005).
RN [9]
RP REPEATS.
RX PubMed=16086015; DOI=10.1038/ng1618;
RA Verstrepen K.J., Jansen A., Lewitter F., Fink G.R.;
RT "Intragenic tandem repeats generate functional variability.";
RL Nat. Genet. 37:986-990(2005).
CC -!- FUNCTION: Component of the cell wall. Required for anaerobic growth.
CC {ECO:0000269|PubMed:11292809}.
CC -!- SUBCELLULAR LOCATION: Secreted, cell wall. Membrane; Lipid-anchor, GPI-
CC anchor. Note=Covalently-linked GPI-modified cell wall protein (GPI-
CC CWP).
CC -!- INDUCTION: Induced during anaerobic growth and by cold shock and
CC glucose. {ECO:0000269|PubMed:11292809, ECO:0000269|PubMed:7746155}.
CC -!- DOMAIN: The number of the intragenic tandem repeats varies between
CC different S.cerevisiae strains.
CC -!- PTM: O-glycosylated. {ECO:0000305}.
CC -!- PTM: The GPI-anchor is attached to the protein in the endoplasmic
CC reticulum and serves to target the protein to the cell surface. There,
CC the glucosamine-inositol phospholipid moiety is cleaved off and the
CC GPI-modified mannoprotein is covalently attached via its lipidless GPI
CC glycan remnant to the 1,6-beta-glucan of the outer cell wall layer.
CC -!- PTM: Covalently linked to beta-1,3-glucan of the inner cell wall layer
CC via an alkali-sensitive ester linkage between the gamma-carboxyl group
CC of glutamic acids, arising from a specific glutamine within the
CC PIR1/2/3 repeat, and hydroxyl groups of glucoses of beta-1,3-glucan
CC chains. {ECO:0000250}.
CC -!- MISCELLANEOUS: Present with 623 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the SRP1/TIP1 family. {ECO:0000305}.
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DR EMBL; X12775; CAA31262.1; -; Genomic_DNA.
DR EMBL; U18778; AAB64544.1; -; Genomic_DNA.
DR EMBL; BK006939; DAA07662.1; -; Genomic_DNA.
DR PIR; S05803; PJBYS.
DR RefSeq; NP_010927.1; NM_001178902.1.
DR AlphaFoldDB; P10863; -.
DR BioGRID; 36742; 22.
DR DIP; DIP-2390N; -.
DR MINT; P10863; -.
DR STRING; 4932.YER011W; -.
DR PaxDb; P10863; -.
DR PRIDE; P10863; -.
DR EnsemblFungi; YER011W_mRNA; YER011W; YER011W.
DR GeneID; 856729; -.
DR KEGG; sce:YER011W; -.
DR SGD; S000000813; TIR1.
DR VEuPathDB; FungiDB:YER011W; -.
DR eggNOG; ENOG502RYU4; Eukaryota.
DR GeneTree; ENSGT00940000176541; -.
DR HOGENOM; CLU_071083_0_0_1; -.
DR InParanoid; P10863; -.
DR OMA; MAYTKIA; -.
DR BioCyc; YEAST:G3O-30198-MON; -.
DR PRO; PR:P10863; -.
DR Proteomes; UP000002311; Chromosome V.
DR RNAct; P10863; protein.
DR GO; GO:0031225; C:anchored component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0071944; C:cell periphery; HDA:SGD.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR GO; GO:0009277; C:fungal-type cell wall; IBA:GO_Central.
DR GO; GO:0000324; C:fungal-type vacuole; HDA:SGD.
DR GO; GO:0005199; F:structural constituent of cell wall; IBA:GO_Central.
DR GO; GO:0031505; P:fungal-type cell wall organization; IBA:GO_Central.
DR InterPro; IPR000992; SRP1_TIP1.
DR InterPro; IPR000420; Yeast_PIR.
DR Pfam; PF00399; PIR; 1.
DR Pfam; PF00660; SRP1_TIP1; 1.
DR PROSITE; PS00929; PIR_REPEAT_1; 1.
DR PROSITE; PS50256; PIR_REPEAT_2; 1.
DR PROSITE; PS00724; SRP1_TIP1; 1.
PE 1: Evidence at protein level;
KW Cell wall; Glycoprotein; GPI-anchor; Lipoprotein; Membrane;
KW Reference proteome; Repeat; Secreted; Signal; Stress response.
FT SIGNAL 1..18
FT /evidence="ECO:0000255"
FT CHAIN 19..233
FT /note="Cold shock-induced protein TIR1"
FT /id="PRO_0000033233"
FT PROPEP 234..254
FT /note="Removed in mature form"
FT /evidence="ECO:0000255"
FT /id="PRO_0000033234"
FT REPEAT 114..119
FT /note="1-1"
FT REPEAT 120..125
FT /note="1-2"
FT REPEAT 126..131
FT /note="1-3"
FT REPEAT 132..137
FT /note="1-4"
FT REPEAT 138..143
FT /note="1-5"
FT REPEAT 144..155
FT /note="2-1"
FT REPEAT 156..167
FT /note="2-2"
FT REPEAT 168..179
FT /note="2-3"
FT REPEAT 180..191
FT /note="2-4"
FT REPEAT 192..203
FT /note="2-5"
FT REPEAT 210..224
FT /note="PIR1/2/3"
FT REGION 110..213
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 114..143
FT /note="5 X 6 AA approximate tandem repeats, Ala/Ser-rich"
FT REGION 144..203
FT /note="5 X 12 AA approximate tandem repeats, Ala/Ser-rich"
FT SITE 220
FT /note="Covalent attachment to cell wall glycan"
FT /evidence="ECO:0000250"
FT LIPID 233
FT /note="GPI-anchor amidated asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 254 AA; 24906 MW; 7BEB8F8399758DEE CRC64;
MAYTKIALFA AIAALASAQT QDQINELNVI LNDVKSHLQE YISLASDSSS GFSLSSMPAG
VLDIGMALAS ATDDSYTTLY SEVDFAGVSK MLTMVPWYSS RLEPALKSLN GDASSSAAPS
SSAAPTSSAA PSSSAAPTSS AASSSSEAKS SSAAPSSSEA KSSSAAPSSS EAKSSSAAPS
SSEAKSSSAA PSSTEAKITS AAPSSTGAKT SAISQITDGQ IQATKAVSEQ TENGAAKAFV
GMGAGVVAAA AMLL
