TIR2_YEAST
ID TIR2_YEAST Reviewed; 251 AA.
AC P33890; D6W277;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1994, sequence version 1.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=Cold shock-induced protein TIR2;
DE AltName: Full=Serine-rich protein 2;
DE AltName: Full=TIP1-related protein 2;
DE Flags: Precursor;
GN Name=TIR2; Synonyms=SRP2; OrderedLocusNames=YOR010C;
GN ORFNames=O2553, UND251;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=7746155; DOI=10.1111/j.1365-2958.1995.tb02248.x;
RA Kowalski L.R.Z., Kondo K., Inouye M.;
RT "Cold-shock induction of a family of TIP1-related proteins associated with
RT the membrane in Saccharomyces cerevisiae.";
RL Mol. Microbiol. 15:341-353(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=YPH148;
RA Donzeau M., Fantino E., Lauquin G.;
RL Submitted (AUG-1993) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8896276;
RX DOI=10.1002/(sici)1097-0061(199609)12:10b<1091::aid-yea22>3.0.co;2-i;
RA Sterky F., Holmberg A., Pettersson B., Uhlen M.;
RT "The sequence of a 30 kb fragment on the left arm of chromosome XV from
RT Saccharomyces cerevisiae reveals 15 open reading frames, five of which
RT correspond to previously identified genes.";
RL Yeast 12:1091-1095(1996).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169874;
RA Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J.,
RA Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A.,
RA Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B.,
RA Dang V.-D., de Haan M., Delius H., Durand P., Fairhead C., Feldmann H.,
RA Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E.,
RA Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U.,
RA Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B.,
RA Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A.,
RA Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C.,
RA Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G.,
RA Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B.,
RA Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B.,
RA Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F.,
RA Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E.,
RA Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I.,
RA Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H.,
RA Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.";
RL Nature 387:98-102(1997).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [6]
RP INDUCTION.
RX PubMed=11292809; DOI=10.1128/jb.183.9.2881-2887.2001;
RA Abramova N.E., Sertil O., Mehta S., Lowry C.V.;
RT "Reciprocal regulation of anaerobic and aerobic cell wall mannoprotein gene
RT expression in Saccharomyces cerevisiae.";
RL J. Bacteriol. 183:2881-2887(2001).
CC -!- FUNCTION: Component of the cell wall. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted, cell wall {ECO:0000250}. Membrane
CC {ECO:0000305}; Lipid-anchor, GPI-anchor {ECO:0000305}.
CC -!- INDUCTION: Induced during anaerobic growth and strongly by cold shock.
CC {ECO:0000269|PubMed:11292809}.
CC -!- PTM: The GPI-anchor is attached to the protein in the endoplasmic
CC reticulum and serves to target the protein to the cell surface. There,
CC the glucosamine-inositol phospholipid moiety is cleaved off and the
CC GPI-modified mannoprotein is covalently attached via its lipidless GPI
CC glycan remnant to the 1,6-beta-glucan of the outer cell wall layer (By
CC similarity). {ECO:0000250}.
CC -!- PTM: Covalently linked to beta-1,3-glucan of the inner cell wall layer
CC via an alkali-sensitive ester linkage between the gamma-carboxyl group
CC of glutamic acids, arising from a specific glutamine within the
CC PIR1/2/3 repeat, and hydroxyl groups of glucoses of beta-1,3-glucan
CC chains. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the SRP1/TIP1 family. {ECO:0000305}.
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DR EMBL; L23521; AAA92869.1; -; Genomic_DNA.
DR EMBL; X74428; CAA52447.1; -; Genomic_DNA.
DR EMBL; U43491; AAC49490.1; -; Genomic_DNA.
DR EMBL; Z74918; CAA99198.1; -; Genomic_DNA.
DR EMBL; BK006948; DAA10793.1; -; Genomic_DNA.
DR PIR; S61310; S61310.
DR RefSeq; NP_014653.1; NM_001183429.1.
DR AlphaFoldDB; P33890; -.
DR BioGRID; 34415; 58.
DR DIP; DIP-4194N; -.
DR IntAct; P33890; 2.
DR STRING; 4932.YOR010C; -.
DR PaxDb; P33890; -.
DR EnsemblFungi; YOR010C_mRNA; YOR010C; YOR010C.
DR GeneID; 854174; -.
DR KEGG; sce:YOR010C; -.
DR SGD; S000005536; TIR2.
DR VEuPathDB; FungiDB:YOR010C; -.
DR eggNOG; ENOG502RYU4; Eukaryota.
DR GeneTree; ENSGT00940000176541; -.
DR HOGENOM; CLU_071083_0_0_1; -.
DR InParanoid; P33890; -.
DR OMA; MAYIKIA; -.
DR BioCyc; YEAST:G3O-33560-MON; -.
DR PRO; PR:P33890; -.
DR Proteomes; UP000002311; Chromosome XV.
DR RNAct; P33890; protein.
DR GO; GO:0031225; C:anchored component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0071944; C:cell periphery; HDA:SGD.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR GO; GO:0009277; C:fungal-type cell wall; IBA:GO_Central.
DR GO; GO:0000324; C:fungal-type vacuole; HDA:SGD.
DR GO; GO:0005199; F:structural constituent of cell wall; IBA:GO_Central.
DR GO; GO:0071497; P:cellular response to freezing; IDA:SGD.
DR GO; GO:0031505; P:fungal-type cell wall organization; IBA:GO_Central.
DR InterPro; IPR000992; SRP1_TIP1.
DR InterPro; IPR000420; Yeast_PIR.
DR Pfam; PF00399; PIR; 1.
DR Pfam; PF00660; SRP1_TIP1; 1.
DR PROSITE; PS00929; PIR_REPEAT_1; 1.
DR PROSITE; PS50256; PIR_REPEAT_2; 1.
DR PROSITE; PS00724; SRP1_TIP1; 1.
PE 2: Evidence at transcript level;
KW Cell wall; Glycoprotein; GPI-anchor; Lipoprotein; Membrane;
KW Reference proteome; Secreted; Signal; Stress response.
FT SIGNAL 1..18
FT /evidence="ECO:0000255"
FT CHAIN 19..231
FT /note="Cold shock-induced protein TIR2"
FT /id="PRO_0000033235"
FT PROPEP 232..251
FT /note="Removed in mature form"
FT /evidence="ECO:0000255"
FT /id="PRO_0000372445"
FT REPEAT 207..225
FT /note="PIR1/2/3"
FT SITE 217
FT /note="Covalent attachment to cell wall glycan"
FT /evidence="ECO:0000250"
FT LIPID 231
FT /note="GPI-anchor amidated glycine"
FT /evidence="ECO:0000255"
FT CONFLICT 69
FT /note="Missing (in Ref. 2; CAA52447)"
FT /evidence="ECO:0000305"
FT CONFLICT 179
FT /note="T -> A (in Ref. 2; CAA52447)"
FT /evidence="ECO:0000305"
FT CONFLICT 192
FT /note="A -> V (in Ref. 2; CAA52447)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 251 AA; 24628 MW; 7B4174FEF5F6415B CRC64;
MAYIKIALLA AIAALASAQT QEEIDELNVI LNDVKSNLQE YISLAEDSSS GFSLSSLPSG
VLDIGLALAS ATDDSYTTLY SEVDFAAVSK MLTMVPWYSS RLLPELESLL GTSTTAASST
EASSAATSSA VASSSETTSS AVASSSEATS SAVASSSEAS SSAATSSAVA SSSEATSSTV
ASSTKAASST KASSSAVSSA VASSTKASAI SQISDGQVQA TSTVSEQTEN GAAKAVIGMG
AGVMAAAAML L
